@GraefLab

We are interested in the #cell_biology of #autophagy, #mitochondria, and #ageing.

 Cornell University (+ MPI-Age)
age.mpg.de/science/research-laboratories/graef/
Joined January 2020
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Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui @Chen3dem using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/ @G__Napolitano, A. Esposito, and A. Ballabio.
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🎉 We're thrilled to share that our former colleague, @_mariyalicheva, now with the @GraefLab, received the prestigious Hans-Grisebach-Preis from @UniFreiburg for her dissertation! 🏅Congrats on this great achievement, Mariya! Photo credit: Universität Freiburg / Jürgen Gocke
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Our work on the biochemical reconstitution of mitophagy initiation led by @Devika_An has been updated on bioRxiv. You can check it out at biorxiv.org/content/10.1101/…
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🚨🚨Come join us! We’re searching for two assistant professor positions in cell and molecular biology, in collaboration with @CornellMBG! 👇
Cornell MBG@CornellMBG17 Sep 2024
Excited to announce an MBG faculty search for two assistant professor positions in collaboration with the Weill Institute for Cell and Molecular Biology, @weillinstitute. Please share and apply! academicjobsonline.org/ajo/j…
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Excited to announce an MBG faculty search for two assistant professor positions in collaboration with the Weill Institute for Cell and Molecular Biology, @weillinstitute. Please share and apply! academicjobsonline.org/ajo/j…
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We’ve worked out how the molecular machines that create acidic compartments can recruit autophagy (self-eating) machinery when they can’t maintain a pH gradient. We are really pleased with this discovery, out in @MolecularCell cell.com/molecular-cell/full…. 1/16

The V-ATPase/ATG16L1 axis is controlled by the V1H subunit

Timimi et al. reveal that the targeting of de-acidified compartments with ATG8s is mediated by a direct interaction between the V-ATPase subunit V1H and ATG16L1. This interaction is important for...

cell.com
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Graef Lab retweeted
OsmanLab@osman_lab5 Aug 2024
🚀Excited to share our latest paper, where we explore dynamic changes in mtDNA heteroplasmy in living cells! Discover how asymmetric partitioning and mitochondrial dynamics drive mtDNA variant segregation! 🔬embopress.org/doi/full/10.10… #Mitochondria #microscopy #microfluidics #mtDNA

Real-time assessment of mitochondrial DNA heteroplasmy dynamics at the single-cell level

The EMBO Journal - Mitochondrial DNA (mtDNA) is present in multiple copies within cells and is required for mitochondrial ATP generation. Even within individual cells, mtDNA copies can differ in...

link.springer.com
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Huge congrats to Sadig @SNiftullayev who has successfully defended his doctoral thesis🥳✨🍻!!! #PhDone @MPIAGE @CGA_age
Cologne Graduate School of Ageing Research@CGA_age15 Jul 2024
🎉 Well done, Dr. Sadig Niftullayev! Today @SNiftullayev, CGA student of the class of 2018, supervised by @langer_lab @MPIAGE, excellently defended his PhD! 👨‍🎓 Time to celebrate 🍾 #PhDone
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Can also find the paper on open access at @CellCellPress cell.com/cell/fulltext/S0092…

MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA...

MTFP1 prevents altered inner mitochondrial membrane subdomains from fusing with the healthier mitochondrial network, enabling their isolation and subsequent autophagic degradation. The findings...

cell.com
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Eat less to live longer? #Autophagy degrades damaged or toxic cellular material to keep cells in good shape. We discovered how #ATG16L1 shapes phagophores. Our article was published today in @NatureSMB ! thanks to all the authors !! Check it out: nature.com/articles/s41594-0…
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🚨The Weill Institute is hiring a new faculty member!🚨 Assistant Professor position is now open in @weillinstitute, joint with @CornellMBG. This is an open search in all areas of molecular and cell biology; application deadline is 3/20. More info: academicjobsonline.org/ajo/j…
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We are happy to share the publication below, which sheds light on the role of Atg1, Atg11 and Atg13 in bulk autophagy initiation. You can find the paper in the following link 👇 pubmed.ncbi.nlm.nih.gov/3823…
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I am thrilled to see our work on the #Vacuole #LipidDroplet #MembraneContactSite vCLIP published in @Dev_Cell! Fantastic collaboration with @ruferbus and @schmidtlabIBK. Huge thanks to the whole team, I am so proud of you all! sciencedirect.com/science/ar…
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Dynamin-like proteins at the nuclear envelope? That and other surprises in excellent work by @PhilipMannino1 with help from @NicholasAder @TJ_MeliaLab and @LuskingL! #autophagy biorxiv.org/content/10.1101/…

A quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like...

Autophagic mechanisms that maintain nuclear envelope homeostasis are bulwarks to aging and disease. By leveraging 4D lattice light sheet microscopy and correlative light and electron tomography, we...

biorxiv.org
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Graef Lab retweeted
JakobsLab@JakobsLab16 Feb 2024
The human prohibitin complex forms a bell-shaped structure in the inner mitochondrial membrane. Check out our #preprint together with @ratz_michael below 👇 biorxiv.org/content/10.1101/… What do you think it looks like? Like a bundt cake? A jellyfish? Vanilla pudding?😉
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I am super excited to share that my first corresponding author paper has been published in JCB (@JCellBiol). Surprising role of ER in ESCRT-mediated sorting! rupress.org/jcb/article/223/… (1/8)
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Molecular simulations, biochemistry, and cell biology come together to reveal how a crucial step in autophagy is executed. The work was made possible through a fantastic synergy between three labs, including ours, located in different continents across the world.
mito911@mito911_7 Feb 2024
Congrats to Dr. Shanlin Rao @HummerLab et al. for revealing in high detail the gymnastic of the LC3 lipidation machinery as it prepares autophagic membranes for cargo engagement using MD simulations, in vitro reconstitution and cell biology science.org/doi/10.1126/scia…

Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the phagophore

Three distinct membrane-docking steps of the ATG12–ATG5-ATG16L1-ATG3–LC3 complex control LC3 lipidation at the phagophore.

science.org
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Graef Lab retweeted
mito911@mito911_7 Feb 2024
Congrats to Dr. Shanlin Rao @HummerLab et al. for revealing in high detail the gymnastic of the LC3 lipidation machinery as it prepares autophagic membranes for cargo engagement using MD simulations, in vitro reconstitution and cell biology science.org/doi/10.1126/scia…

Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the phagophore

Three distinct membrane-docking steps of the ATG12–ATG5-ATG16L1-ATG3–LC3 complex control LC3 lipidation at the phagophore.

science.org
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Exciting discoveries from @ShoSuzuki20 in the Emr lab — bringing it full circle back to the classic Emr lab vps screen: a mechanism for how intraluminal vesicles formation steals membrane from the limiting membrane yet the organelle doesn’t shrink — VPS13 supplies new lipids!
Henne_Lab@HenneLab6 Feb 2024
Exciting work from @ShoSuzuki20 in the Emr lab exploring functional crosstalk between Vps13 and ESCRT machinery in ER-endosome lipid homeostasis. rupress.org/jcb/article/223/…

A role for Vps13-mediated lipid transfer at the ER–endosome contact site in ESCRT-mediated sorting

Suzuki et al. reveal a critical role for Vps13-mediated lipid transfer at ER–endosome contact sites in ESCRT-mediated sorting, elucidating the contribution

rupress.org
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Ever wondered how cells adapt #autophagy to various forms of stress?⚡️ So happy to share our story on the plasticity of the Atg1 kinase complex in response to #phosphate starvation. For more details read here 🧵 and here nature.com/articles/s41556-0…
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