@FPbase

The Fluorescent Protein Database. tweets by @TalleyJLambert

fpbase.org
Joined January 2019
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  • Followers1.5K
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This exercise was less informative than I'd hoped, but perhaps it's interesting to someone else: spectroscopically-inferred estimates of densities of states for a selection of fluorescent proteins spanning the visible spectrum. Data from @FPbase.
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Mutagenesis of #FbFP #LOV domain: 🟠 22 variants with 486-512 nm emission maxima 🟠 three-channel imaging via spectral unmixing 🟠 two-channel fluorescence lifetime unmixing doi.org/10.1101/2022.12.09.5… Congratulations, @ivangushchin, @Aynya5, and all the authors! @FPbase
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Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at @naturemethods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵nature.com/articles/s41592-0…

Chemically stable fluorescent proteins for advanced microscopy

Nature Methods - The engineered hyperfolder YFP (hfYFP) and variants offer unprecedented chemical and thermal stability, making them versatile probes for microscopy as well as for challenging...

nature.com
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this is genius: @helsinkiuni BioImaging Unit utilizes @FPbase to allow users test their fluorophores fpbase.org/microscope/u4gqNc… cc @Daniel_Wa19
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🥁 Delighted to announce that our FPCountR paper is now on bioRxiv! In this paper we describe the development of a method to allow absolute fluorescent protein quantification using microplate readers for synthetic circuit characterisation. biorxiv.org/content/10.1101/… 🧵
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Just discovered the interactive chart of FPbase.com. Wow, what an amazing tool to compare different properties across a wide range of fluorescent proteins. Great work @TalleyJLambert 🙂 fpbase.org/chart/
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Replying to @miCHRIScopy @TalleyJLambert
FPbase is amazing! I created scope configurations for @UAZCancer core instruments, and link to it with a little QR code posted next to the scope! And made a nice lab exercise for my microscopy class. iScopeCalc rocks too. Keep ‘em coming, @TalleyJLambert! – at Arizona, USA
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📣 Apply for an award from Addgene to attend a conference! We're accepting applications until April 30 ☑️ Undergrad, grad student, postdoc, research tech ☑️ Working w/ fluorescent proteins ☑️ Planning to attend a conference, virtual, hybrid, or in-person hubs.ly/H0LbWNd0

Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

See the winners for the 2021 Michael Davidson and Roger Tsien Commemorative Conference Award.

blog.addgene.org
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Replying to @TalleyJLambert @edward_marcotte @J_A_C_S
more JF spectra than you can shake a stick at. (2P coming soon) thanks @rhodamine110! fpbase.org/spectra/?s=6373,6…
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Just added our new GZnP3 sensor to #FPbase: The Fluorescent Protein Database. Follow @FPbase for updates on newly added FPs. fpbase.org nature.com/articles/s41467-0…

FPbase: The Fluorescent Protein Database

FPbase is a free and open-source, community-editable database for fluorescent proteins and their properties.

fpbase.org
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New microcourse! Can you confidently choose the optimal filter set for imaging your fluorophore? This video walks you through the process. Enjoy! youtu.be/9o5E27dnauk

Fluorescence Filters: Choosing a Filter Set

Can you confidently choose an optimal filter set to image your fluo...

youtube.com
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A really nice blog post on our preprint, thanks @preLights !
preLights@preLights8 Jul 2019
‘Far beyond your average(Av)GFP - Aequorea victoria’s secret fluorophores revealed’. The @NathanShaner Lab uncovers several new fluorophores from A. victoria and A. australis - including the brightest GFP homolog yet! prelights.biologists.com/hig… by #preLighter @somssichm
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Great paper out today in @naturemethods from the Dedecker and Bourgeois labs on using blue light to reduce/eliminate dark states in mEos4b for improved single particle tracking nature.com/articles/s41592-0…
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Check out @FPbase for all your fluorescent protein questions! Using FPbase.org and the NIH's nucleotide blast, our lab just discovered that many of our EYFP constructs are actually Venus.
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Tweet me a name of a Fluorescent Protein and I will (try to) respond with an advantage and a shortcoming. I'll go first: #mTurquoise2 @joachimgoedhart xitter.cf/DrGriff34/status/11132…
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And it is done :)
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From now on, also the (mNeon)green variants of "Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells" are available from @addgene: addgene.org/browse/article/2… biorxiv.org/content/10.1101/…

Addgene

addgene.org
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Choosing fluorescent proteins has never been easier thanks to FPbase! Learn more nature.com/articles/s41592-0… rdcu.be/brZJF @FPbase @TalleyJLambert
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This is the first resource we will share with our users: FP database is very comprehensive and user friendly! You can search the right fluorescent protein for your experiment easily with this tool. Hopefully the community keeps it thriving by adding to it.
FPbase@FPbase9 Jan 2019
The Fluorescent Protein Database has a twitter account! Follow @FPbase to stay up to date on newly-published #fluorescentproteins and FPs recently added to the database at fpbase.org Recent activity at the database can also be found here: fpbase.org/activity
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FPbase retweeted
Addgene@Addgene7 Jan 2019
Announcing our 2019 Michael Davidson and Roger Tsien Commemorative Travel Awards! If you're a grad student or postdoc using #fluorescentproteins in your research, consider applying! More details below. #phdchat #ECRchat hubs.ly/H0g4pCz0
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