@FPbase

The Fluorescent Protein Database. tweets by @TalleyJLambert

fpbase.org
Joined January 2019
  • Tweets129
  • Following1
  • Followers1.5K
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Try the new FPbase spectra viewer! Completely rewritten to be faster, more customizable, and full-featured: • SVG/PNG/CSV export • state recovery & URL sharing • keyboard shortcuts for rapid entry 💚 • efficiency calculations • full documentation fpbase.org/spectra

FPbase Fluorescence Spectra Viewer

An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.

fpbase.org
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Hi Fluorescence friends! Please follow future updates on Bluesky: bsky.app/profile/fpbase.org
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This exercise was less informative than I'd hoped, but perhaps it's interesting to someone else: spectroscopically-inferred estimates of densities of states for a selection of fluorescent proteins spanning the visible spectrum. Data from @FPbase.
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recently hit 2,000,000 sessions on @FPbase ! 📈
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Mutagenesis of #FbFP #LOV domain: 🟠 22 variants with 486-512 nm emission maxima 🟠 three-channel imaging via spectral unmixing 🟠 two-channel fluorescence lifetime unmixing doi.org/10.1101/2022.12.09.5… Congratulations, @ivangushchin, @Aynya5, and all the authors! @FPbase
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Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at @naturemethods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵nature.com/articles/s41592-0…

Chemically stable fluorescent proteins for advanced microscopy

Nature Methods - The engineered hyperfolder YFP (hfYFP) and variants offer unprecedented chemical and thermal stability, making them versatile probes for microscopy as well as for challenging...

nature.com
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Chapter on using FPbase tools out now in the MMB issue on FPs. Thank you for editing @mayankdu!
Mayank Sharma@mayankdu15 Sep 2022
Happy to share the Methods in Molecular Biology issue on Fluorescent Proteins 🌈🎉😀. Including many “much awaited” protocols on and about Fluorescent Proteins/applications. A big THANKS to authors across the globe for contributing. 👏🏻💐 link.springer.com/book/10.10…

Fluorescent Proteins

link.springer.com
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this is genius: @helsinkiuni BioImaging Unit utilizes @FPbase to allow users test their fluorophores fpbase.org/microscope/u4gqNc… cc @Daniel_Wa19
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anyone out there routinely express FPs in Chlamydomonas? any modern suggestions for SNR? (presumably red, but any expression gotchas?)
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🥁 Delighted to announce that our FPCountR paper is now on bioRxiv! In this paper we describe the development of a method to allow absolute fluorescent protein quantification using microplate readers for synthetic circuit characterisation. biorxiv.org/content/10.1101/… 🧵
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Always nice to see features put to use! @eszter_cs and @gbstan use the FPbase API (among other things) in a method for the calibration of fluorescence readings on microplate readers; for quantification of FP concentration biorxiv.org/content/10.1101/…

FPCountR: Absolute quantification of fluorescent proteins for synthetic biology

This paper presents a generalisable method for the calibration of fluorescence readings on microplate readers, in order to convert arbitrary fluorescence units into absolute units. FPCountR relies on...

biorxiv.org
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Just discovered the interactive chart of FPbase.com. Wow, what an amazing tool to compare different properties across a wide range of fluorescent proteins. Great work @TalleyJLambert 🙂 fpbase.org/chart/
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Replying to @miCHRIScopy @TalleyJLambert
FPbase is amazing! I created scope configurations for @UAZCancer core instruments, and link to it with a little QR code posted next to the scope! And made a nice lab exercise for my microscopy class. iScopeCalc rocks too. Keep ‘em coming, @TalleyJLambert! – at Arizona, USA
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Writing a methods chapter on using FPbase for an upcoming MMB book... Will include things like -Browsing/searching FPs on fpbase -Spectra viewer -microscope & reports feature If there's an aspect or detail of FPbase that you wish were better documented, let me know!
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(existing docs are at help.fpbase.org/)

FPbase Help

Thank you for using FPbase! Use the table of contents to the left, or the search bar at the top to find what you're looking for.

help.fpbase.org
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This is beautiful and makes me want to rewrite FPbase using it 😍
Sebastián Ramírez@tiangolo24 Aug 2021
Big project, first public release! 📢 ✨ SQLModel ✨ ...the biggest thing I've built since FastAPI & Typer. 🤓 SQL DBs based on Python type hints. 😎 Each model is both a Pydantic and @sqlalchemy model. 🤯 Optimized for FastAPI 🚀 github.com/tiangolo/sqlmodel

GitHub - fastapi/sqlmodel: SQL databases in Python, designed for simplicity, compatibility, and...

SQL databases in Python, designed for simplicity, compatibility, and robustness. - fastapi/sqlmodel

github.com
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Dear imaging companies, Please tell your customers (or make available on request) the *actual* spectra for all components (LEDs, filters, etc) in their light path. just saying "460-490nm" is not sufficient. It's amazing how many scientists can't get this info from their reps 😞
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a reminder on the difficulty in comparing fluorescence photobleaching measurements across studies (and even across probes on the same scope) fpbase.org/bleaching/

FPbase: The Fluorescent Protein Database

FPbase is a free and open-source, community-editable database for fluorescent proteins and their properties.

fpbase.org
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📣 Apply for an award from Addgene to attend a conference! We're accepting applications until April 30 ☑️ Undergrad, grad student, postdoc, research tech ☑️ Working w/ fluorescent proteins ☑️ Planning to attend a conference, virtual, hybrid, or in-person hubs.ly/H0LbWNd0

Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

See the winners for the 2021 Michael Davidson and Roger Tsien Commemorative Conference Award.

blog.addgene.org
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Databases are hard... and no amount of automated error checking can catch every mistake that humans make (in the literature or in submissions). FPbase gratefully accepts help/corrections from anyone! Here's an example of what not to do if you find something needing correction 😂
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Replying to @TalleyJLambert @edward_marcotte @J_A_C_S
more JF spectra than you can shake a stick at. (2P coming soon) thanks @rhodamine110! fpbase.org/spectra/?s=6373,6…
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