@ratherbecloning

Microscopist, plasmid enthusiast, Advanced Imaging Specialist @Nikoninst. Posts are my own.

 Tucson, Arizona
Joined April 2018
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Parker @ratherbecloning et al. @mouneimnelab @UArizonaCMM show that the Ena/VASP protein, EVL, complexes with the I-BAR protein, MIM/MTSS1, to drive filopodial protrusion from dendrites during neurodevelopment. bit.ly/41sksNR #Cytoskeleton #Neuroscience
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Pro tip: in a pinch, a barrel cactus makes an excellent tripod. Happy New Year all!
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#Microscopy and #CellBiology tweeps. Thinking of getting a tattoo on my shoulder of an epithelial monolayer with cells migrating away, actin stain for sure. Share your best actin images for inspiration!! – at Tempe, AZ
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@chillinwithpfn1 I bet you have some great ones! – at Tempe, AZ
When you don’t fully understand how Dronpa works the first time you use it 😝
Microscopy Memes 🔬@MicroscopyMemes6 Dec 2021
Reply/retweet with your own caption!
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Truth @NikonInst #microscopy – at Tempe, AZ
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My second office, for those rare times when I only need one monitor!
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What an office…
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Learning SO MUCH about JOBS and GA3 in my first week at @NikonInst. @mouneimnelab can I get a redo on my postdoc? No more manual tracking for me! – at Arizona, USA
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Last day @mouneimnelab, and I’ve already done a transfection for a weekend IP, plated neurons, probed a WB, done mini preps, ran a PCR, digested said PCRs, collected lentivirus, and it’s not even lunchtime! And I might never do these bench activities again. Kinda crazy! – at Tucson, AZ
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Recombination was the bane of my existence for my first year of post-dockin’, don’t let it happen to you! #molecularbiology blog.addgene.org/preventing-… – at Arizona, USA

Three Tips for Preventing Viral Plasmid Recombination in Your Samples

Having trouble with viral vectors? Here are several tips to help prevent recombination in viral vectors or other plasmids with repeating elements.

blog.addgene.org
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Also, multi-site Gateway is the worst
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Tying up loose ends at the @UAZCancer microscopy core before I head to @NikonInst next week! I'll miss my scope love logo
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This week, I had what would be (unless I pick up some freaky hobbies) almost certainly the last dissection of my life. I am super-excited to say that I am starting a position with Nikon Instruments, and leaving research behind. The job is remote, so I’m extremely lucky to be… 1 – at Arizona, USA
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Academic research and our training selects for people who can run the gauntlet again and again and again, and having run this gauntlet countless times, and only seeing more gauntlets on the horizon, I’m calling it. In spite of my gripes, I’m extremely grateful to be the…6 – at Arizona, USA
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thinking person I am today, and to have made so many amazing friends from all over the world! 💚❤️💚 This position puts me in the middle of the future of microscopy - quantitative biology, AI-enhanced image analysis, software development. I could not be more excited 🔬 7 – at Arizona, USA
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