The Fluorescent Protein Database. tweets by @TalleyJLambert

Joined January 2019
They all retain the alias mStayGold, and so will all still show up when one searches for "mStayGold"
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recently hit 2,000,000 sessions on @FPbase ! 📈
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Replying to @FPbase @bakermind
took a look, there are 90 2P spectra currently available in the database. So, this is a user interface problem rather than a data storage problem 😬 ... (GM units are also stored in the database, but easily accessible) my apologies!
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Databases are hard... and no amount of automated error checking can catch every mistake that humans make (in the literature or in submissions). FPbase gratefully accepts help/corrections from anyone! Here's an example of what not to do if you find something needing correction 😂
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FPbase has been on Twitter for 1 year! And just last week we hit 2,000 registered users! Thanks for all your contributions and great suggestions 🎉 keep ‘em coming
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Annoyed with the similar colors of neighboring spectra on the FPbase viewer? Be annoyed no more! New color palettes added in the settings menu (cycle with the C key) Thanks @JonasRies for the suggestion, and @joachimgoedhart for some colorblind friendly palette inspiration.
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We want the FPbase spectra viewer to be your one-stop spectra shop :) currently 250+ proteins, 540+ dyes, 2760+ filters and now with a quick lookup for all common *filter sets* from Chroma, Semrock, Omega, and (newly added) Zeiss. just hit spacebar... help.fpbase.org/tools/spectr…
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yeah that's what's so interesting! in most cases, it seems like there's some explanation other than codon usage (as @hsalis already said above). kinda fascinating for such a ubiquitous practice. I was also struck by fig 2 in this 2009 paper with GFP: ncbi.nlm.nih.gov/pmc/article…
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Even the best fluorophores are only useful if they actually match the filters on your system. Introducing efficiency reports at FPbase: evaluate ex/em efficiency for every fluorophore in the database, with each config on your scope. Create your own here: fpbase.org/microscopes/
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Replying to @lesloew
glad you find it useful! If you frequently make FRET calculations, don't miss the fret page at fpbase: fpbase.org/fret
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