@Addgene

Sharing speeds science! Addgene helps scientists around the world find and share research materials - plasmids, viral preps, antibodies, data, and more.

 Watertown, MA
addgene.org
Joined October 2009
  • Tweets39.7K
  • Following8.6K
  • Followers21K
14.4KMedia
Addgene is no longer monitoring or using our X, formerly Twitter, account. Instead, find @addgene on Instagram, TikTok, Facebook, LinkedIn, and emerging microblogging platforms. You can read more about this decision here: blog.addgene.org/addgene-is-…
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🎊 Happy birthday, @Addgene! 🥳 Today we cell-ebrate you and the 137,289 plasmids you have sent on behalf of 5,822 labs from around the world!🤯 Get to know Addgene's #OpenScience mission: addgene.org
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Our Cell Line of the Week is SERCA2 - illuminating the sarcoplasmic/ endoplasmic reticulum! 💪 Browse our openly available hiPSC lines & plasmids – fluorescently-tagged to illuminate structures: bit.ly/AccessAllenCellCollec… 📦 Distributed by @Coriell_Science & @Addgene

SERCA2 | Allen Cell Collection

This time-lapse movie shows beating hiPSC-derived cardiomyocytes expressing mEGFP-tagged SERCA2 protein.

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The key reagents from our recent CombiCell manuscript are now available on @Addgene addgene.org/Omer_Dushek/ @embojournal embopress.org/doi/full/10.10…
Omer Dushek@Dushek
19 Jun 2023
Our new work! Using CombiCells, a platform enabling titration and combinatorial display of cell surface ligands, to investigate the sensitivity and costimulatory requirements of TCRs and CARs biorxiv.org/content/10.1101/…
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Working with @addgene to make all plasmids easy to get soon
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Our #CellLineOfTheWeek is ras-related protein - targeting endosomes! 🧫 Our hiPSC lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCellCollec… 📦 Distributed by @Coriell_Science & @Addgene #OpenScience

Ras-related protein | Allen Cell Collection

Timelapse movie of live hiPS cell colony expressing one copy (monoallelic) of mEGFP-tagged Ras-related protein Rab-5A.

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14. Get your SOXmas presents @Addgene addgene.org/browse/article/2…
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Most of our pHYX plasmids recently published doi.org/10.1016/j.ymben.2023… are now available @Addgene (addgene.org/browse/article/2…). And merry Christmas to all! 🎅🎄 @ECCP_lab
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We've previously reported PAM variable PEmax for correcting KRAS mutations, and now our constructs - PEmax-SpG, PEmax-SpG-P2A-hMLH1dn - are available on @Addgene! Check them out! #CRISPR #PrimeEditing addgene.org/browse/article/2…
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Troponin I (slow skeletal) is our #CellLineOfTheWeek! ❤️🔬 Our human iPS cell lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCellCollec… 📦 Distributed by @Coriell_Science & @Addgene. #OpenScience

Troponin I, slow skeletal | Allen Cell Collection

This time-lapse movie shows live hiPSC-derived cardiomyocytes expressing mEGFP-tagged troponin I, slow skeletal muscle.

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Perspective (OA): "The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene" liebertpub.com/doi/10.1089/c… >15,000 plasmids from >1,000 laboratories ∼40 countries 300,000 shipments to ∼5,000 organizations in ∼100 countries @Addgene @CRISPRchef
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Haemophilus influenzae was the first bacteria to be sequenced but few genetic tools exist for this organism. It has small genome of only 1.8 Mbp and can be easily grown in lab. To speed up synbio research for this organism we created plasmids that now you can get @Addgene
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Addgene retweeted
Quoc@quoctran98
12 Dec 2023
We're depositing parts on @Addgene and hope that our tools join the MoClo-YTK ecosystem to make yeast synbio more accessible, alongside recent work from @willmshaw, @MoKhalilLab, and @ProfTomEllis (pubs.acs.org/doi/10.1021/acs…), whose previous tools have enabled a lot of our work.

A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae

Abstract. Synthetic biology toolkits are one of the core foundations on which the field has been built, facilitating and accelerating efforts to reprogram

pubs.acs.org
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You can find us on: Instagram: hubs.la/Q02cTfDN0 TikTok: hubs.la/Q02cT7wG0 Facebook: hubs.la/Q02cT7pp0 LinkedIn: hubs.la/Q02cTctc0 Addgene Blog: hubs.la/Q02cT8570
Blugene standing outside under a blue skye, with the works "Find Use @addgene.bsky or @addgene Instagram, Facebook, TikTok, and LinkedIn

ALT Blugene standing outside under a blue skye, with the works "Find Use @addgene.bsky or @addgene Instagram, Facebook, TikTok, and LinkedIn

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Click editor plasmids now available on @Addgene addgene.org/browse/article/2… 🧬🖱️ Looking forward to feedback from the community on how well click editing works in your hands! @FerreiraSilvaJ @connorjtou @EmilyM_King @Madeline_Eller_ @Linyuan79834832 @DavidRufinoRam1
Ben Kleinstiver@BKleinstiver
14 Sep 2023
We’re excited to share our work developing a new gene editing approach that we’re calling #clickediting 🖱️✴️🧬. Click editors (CEs) utilize DNA-dependent polymerases to write bespoke edits into the genome. biorxiv.org/content/10.1101/… @CGM_MGH @MassGeneralNews @MGH_RI @harvardmed

Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases

Genome editing technologies that install diverse edits can widely enable genetic studies and new therapeutics. Here we develop click editing, a genome writing platform that couples the advantageous...

biorxiv.org
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The plasmids are available on @Addgene, including recombinases, reporters, and empty vectors for your own favorite gene: addgene.org/browse/article/2…
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Interested in Transcription Factor high-content screening? we have a dFLASHy new platform for you. dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Plasmids @Addgene biorxiv.org/content/10.1101/…

dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell...

Live-cell reporting of regulated transcription factor (TF) activity has a wide variety of applications in synthetic biology, drug discovery, and functional genomics. As a result, there is high value...

biorxiv.org
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Our #CellLineOfTheWeek is sialyltransferase 1 - targeting the golgi! 🧫 Our hiPSC lines & plasmids – fluorescently-tagged to illuminate structures – are openly available to accelerate discovery: bit.ly/AccessAllenCellCollec… 📦 Distributed by @Coriell_Science & @Addgene #OpenScience

sialyltransferase 1 | Allen Cell Collection

Timelapse movie of a live hiPS cell colony expressing one copy of mEGFP tagged sialyltransferase 1. Images were taken in 3D every 3 minutes for 6 hours on a spinning-disk confocal microscope.

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Update: most of the Contact-FP probes are now available on @Addgene. We hope folks will try them out and let us know what you think! addgene.org/Sarah_Cohen/
Sarah Cohen@cohenlaboratory
28 Sep 2023
Preprint alert! We are happy to introduce Contact-FP, a suite of probes for studying #MembraneContactSite dynamics. This was an amazing team effort led by Research Associate @GregoryMiner18 and postbacc Sidney Smith 1/11 biorxiv.org/content/10.1101/…

Contact-FP: a dimerization-dependent fluorescent protein toolkit for visualizing membrane contact...

Membrane contact sites (MCSs) are sites of close apposition between two organelles used to exchange ions, lipids, and information. Cells respond to changing environmental or developmental conditions...

biorxiv.org
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🌈plasmids now available at @addgene 🍭 addgene.org/Arnaud_Gautier/
Franck Perez@Team_FranckP
28 Aug 2023
Very happy to share a new approach we developed at @institut_curie/@CNRS with my colleague and friend Arnaud Gautier from @Sorbonne_Univ_/@ENS_ULM CATCHFIRE allows to induce and sense protein interaction using a reversible fluorogenic system. More here : rdcu.be/dkAMo
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