ALT Live-imaging of a LifeAct-RFP embryo from LB to LHF stage, showing actin cytoskeleton changes over the lip of the foregut pocket and condensation over the notochord (yellow arrows). Scale bar 50 µm.

ALT Outcomes from the extended Fast & Fair trial showing (A) faster time to first decision compared to conventional peer review and (B) that review quality was not compromised

ALT SPOTTER Proteomic Workflow. A) Set-up of SPOTTER for Direct Labeling of Tissue Proteins. SPOTTER integrates two functionally synchronized subsystems: a high-pressure nanoflow pump system for precision reagent delivery and a 3D printing platform for spatially resolved depositions, in which the thermoplastic extrusion assembly was replaced with a custom-made fused silica nano-flow liquid chromatography column as a microfluidic deposition nozzle. B) Murine brain atlas with region-to-tag assignments. Ten mouse brain regions were outlined and color-coded to illustrate their corresponding TMTpro-18plex tag assignments. C) SPOTTER workflow: (1) Annotation of brain regions on tissue sections. (2) Region-specific on-slide labeling using a custom printing pattern. (3) Tissue lift-off, lysis, and digestion. (4) Peptide-level TMT post-labeling to correct for unlabeled background. (4) Fractionation of labeled peptides. (5) Parallel LC-MS/MS analysis for global proteome and IMAC-enriched phosphoprot

ALT Illustration of three morphogenetic movements during Drosophila gastrulation (left). Traces from live embryos showing cell contact changes during germband extension, mesoderm invagination, and neuroblast ingression. Germband extension and neuroblast ingression involves the shrinkage and collapse of cell-cell contacts and the formation of new junctions illustrated in blue and red, respectively. In contrast, of the invagination of the mesoderm relies on apical constriction without significant cell contact changes.