Postdoc in the #MougousLab studying bacterial antagonism | Looked at bacterial chromatin and gene expression for my PhD in @LandickLab | the science of cooking

Seattle, WA
Joined April 2012
Excited to share a preprint with some of my thesis work done in the @LandickLab! We used cool techniques to unravel connections between H-NS filament structure and DNA sequence. Thanks to my co-authors for making this project fun! @cmhustmyer @mikebwolfe @JessenErik
Hybrid analysis reveals how DNA sequence governs genomic location and DNA contacts of bacterial chromatin H-NS filaments biorxiv.org/cgi/content/shor… #bioRxiv
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Very honored to be selected as an LSRF fellow and to be joining this community! Thank you @LSRFdtn and @HHMI for the opportunity, and to all of my mentors, especially Joseph Mougous and @DWeissLab for the support and encouragement! Congrats to my fellow awardees 🎉
Finally! It's been hard to keep this under wraps until August 1st. We are excited to announce our 2022 awardees. Our entire current awardee group is listed on our website: lsrf.org/current-awardees #lifescience #postdoc #earlycareer #funding #basicresearch, #postdocfunding, #award
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One thing I love about my postdoc is learning new techniques! Today I got to add long-read sequencing (@nanopore) to my list. Never thought that would happen. Very excited for the data I can get from this small wonder. 🎉🦠🧬
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Does anyone have experience with inducible plasmids for protein expression in gram-positives they could share with me? Not picky about the organism or the inducer/plasmid system, just curious about experiences. DM me if you have a lot of thoughts. TIA! 🦠🧬
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This type of content is why I am on twitter. Really hilarious stuff. original comic from one of my favs: @xkcd.
Types of Microbiology paper. 🔬
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Herman Muller and colleagues doing Drosophila genetics. Via IU Biology, IU Lilly Library. THERE IS A CAT ON THE TABLE.
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I love these so much!😂
every year I make low effort chemistry valentines in comic sans for my coworkers
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Congrats to my PI for this award for some amazing science! 🎉🎉
Replying to @hhmi_science
Joseph Mougous has been awarded the 2021 @theNASciences Award in Molecular Biology for his pioneering work in microbiology: bit.ly/2XYtF15
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I love Molecule of the Month! An easy (and pretty!) way to learn about diverse structures solved over the years. Congrats on 20 years! 🥳🦠🔬🧬
In 2020, we celebrated twenty years of Molecule of the Month articles by @dsgoodsell. This series that has introduced millions to the shape and function of the 3D structures archived in the PDB pdb101.rcsb.org/motm/motm-ab…
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😍😍😍
Aaaannnddd, the 1st place winner in the #AgarArt Traditional category is “The Gardener” by Joanne Dungo from Northridge Hospital Medical Center in Northridge, CA. skyw.io/UGubFe
Should online labs be more like cooking shows? Lots to ponder about what students should take away from a lab course in this article.
How can we design practical online labs that enable students to experience the consequential, causal links characteristic of our best in-person labs? Pete Kenelly has some suggestions, inspired by Julia Child: asbmb.org/asbmb-today/career….
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I love these so much.Kind of how I envision all my bacteria on plates anyway.😍🦠
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Literal energy for today. But seriously, what an awesome molecular machine!
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I'm thinking about a new cloning project and wondering how people pick RE to use. Is there a secret "go-to" list of RE that I don't know about? Share your RE thoughts with me, please! 🧬🦠😀
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I got advice one time that "if you've heard of it, it's a good enzyme." I never liked that advice, but never got anything better.
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The other day I was talking with a friend about new findings and techniques that have revolutionized science since we started grad school (only 8 years ago!). I feel really privileged to have witnessed some of these changes. #cryoEM is one of those techniques. (1/2)
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I bring this up because I’m so impressed by the recent publications showing structures of RNAP-ribosome complexes. With cryoEM, the past few years have revealed key insights (and brought up more questions!) about how this interaction affects bacterial gene expression. (2/3)
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Can’t wait to see what the future of cryoEM brings to understand cool biology of bacteria and beyond! (3/3)
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More views of the transcription-translation complex! Great work!
Paper from our group presents cryo-EM structures that show how the fundamental, "central-dogma" biological processes of transcription and translation are coupled in the bacterium Escherichia coli. Structural basis of transcription-translation coupling science.sciencemag.org/conte…
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Cool work showing structural insight into transcription and translation coupling, including impressive analysis of this dynamic structure.
We are very excited to see our #cryoEM paper from @AlbertW_Lab on the structural basis of transcription-translation coupling is online now in @ScienceMagazine: science.sciencemag.org/conte… The following thread summarises what it shows:
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Cheering for my friends in the @LandickLab who characterized metabolic flux in a promising microbe for bioproduction. Foundational work to help get microbes to work for us!
Remember learning about pyruvate decarboxylase (PDC) in biochem class? Turns out PDC levels can steer carbon flux toward useful bioproduct production in Zymomonas mobilis, a natural ethanol producer. Check it out here: sciencedirect.com/science/ar…
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