@think_zesty

One step closer to becoming a biochemist, but physical sciences are also zesty sciences. Structural biology is the toastiest field. She/Her 🇨🇦

 Toronto, Ontario
Joined July 2017
  • Tweets73
  • Following199
  • Followers79
Happy to share my first PhD paper from @PLynneHowell lab with my fantastic post-doc mentor @gregbwhitfield from @BrunLabCaulo as well as @Dr_Lori_Burrows and @RubinsteinJohn with #cryoem support where we propose how the bifunctional Tad pilus motor works🧵 biorxiv.org/content/10.1101/…
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nature.com/articles/s41586-0… We are so thrilled to share our latest research! We revealed a new and intriguing GPCR activation mechanism from the intracellular agonist.

Class B1 GPCR activation by an intracellular agonist

Nature - A new intracellular agonist-binding pocket is identified that is common to many G protein-coupled receptors, which will have implications for the development of biased compounds that...

nature.com
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In new work, Michon et al. study the role of detergents and detergent substitutes in cryo-EM grid preparation for high-resolution structural studies of membrane proteins. Check it out here: bit.ly/3LVzPaO @IPGP_officiel @sacquin_mo @UGrenobleAlpes

Role of surfactants in electron cryo-microscopy film preparation

Single-particle electron cryo-microscopy (cryo-EM) has become an effective and straightforward approach to determine the structure of membrane proteins. However, obtaining cryo-EM grids of sufficient...

cell.com
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My thesis examiners passed my PhD thesis with ZERO corrections. One of their comments was that 'it was extremely well written'. How? By following a strict consistent structure throughout. This is exactly how you should structure your results chapter:
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For centuries, certain groups have been falsely labeled as “more human.” Harnessing data from >60k ps across 13 studies, @banaji, @MaddBlackProf & I explored whether all racial/ethnic groups are automatically associated with the concept of “Human.” Here are the 7 key results...
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Excited to share my first scientific manuscript out now on @biorxivpreprint, where we present the first ever #cryoEM structure of the iron #nitrogenase complex. Let me tell you about our newest insights into the mystery of biological nitrogen fixation: doi.org/10.1101/2023.05.02.5…
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Incredibly useful "cheatsheet" on cancer chemotherapy classes. From Cell Press sciencedirect.com/science/ar…
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A native mass spectrometry method enables analysis of membrane protein-lipid complexes under near-physiological conditions, to determine oligomeric states, identify bound lipids and membrane properties. @GuptaLab1 @YaleCellBio @anipan84 @YaleWestCampus nature.com/articles/s41592-0…
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Team headed by Christian Baron @med_umontreal and Khanh Huy Bui @McGillMed determined the cryo-EM structures of two type IV secretion system-determined pili cell.com/structure/fulltext/…
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🧵Very pleased to share my 3rd first author paper & 1st project during my time in grad school in the @PLynneHowell lab, published in @JournalSpectrum journals.asm.org/doi/10.1128… on identifying a Pel biofilm inhibitor via high throughput screening.

Small Molecule Inhibition of an Exopolysaccharide Modification Enzyme is a Viable Strategy To Block...

Present in more than 500 diverse Gram-negative and 900 Gram-positive organisms, the Pel polysaccharide is one of the most phylogenetically widespread biofilm matrix determinants found to date....

journals.asm.org
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Another Contractile Injection System? 💉🙄 BUT THIS is a special one 👈😱 Spearheaded by our postdoc @casub1 in a fantastic collab with @JWSallmenII & @SusanSchlimpert, they could demonstrate a cytoplasmic role (☠️) of CIS in #Streptomyces out now in @NatureMicrobiol 🧵1/2
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Caution for mCherry users: alternate start @ M10 (from internal SD seq.) leads to undesired free FP in constructs w/C-term mCherry fusions, but M10L/Q can fix it. See this 2022 paper: frontiersin.org/articles/10.… @joachimgoedhart @NathanShaner @Campbell_FPs—thoughts? (h/t @MasaakiU5)
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Battling biofilm to prevent dangerous lung infections: Researchers @uoft and @SickKidsNews have identified a promising therapeutic target to help treat lung infections in #cysticfibrosis patients. @UofTArtSci #CF Read the full story: lightsource.ca/public/news/2…
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I think the scientific community should sometimes take a minute to acknowledge/appreciate the extra work, time & energy that students + researchers around the world, whose native language is not English, need to put into writing academic papers + giving talks in English. 👇🧵 1/7
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John Rubinstein's lab @UofTB and collaborators solved the cryo-EM structure of lemon V-ATPase. @RubinsteinJohn @tanyongzi doi.org/10.1016/j.str.2022.0…
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TomoTwin official release pt 1 🥳! After some code streamlining and writing documentation we’ve released the first stable build of TomoTwin. Here’s an example of TomoTwin picking Tc toxins based on a single reference without having to be trained on the protein beforehand. 🧵1/6
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NikA continues to surprise! Check out this paper in @Metallomics from Wayne Law in the Zamble and @KanelisLab @UofTChem showing that, although NikA binds lots of metals, it loves Ni(II)! But NikA can also change its metal selectivity upon H416 mutation. (academic.oup.com/metallomics…)

Biochemical studies highlight determinants for metal selectivity in the Escherichia coli periplas...

Abstract. Nickel is an essential micronutrient for the survival of many microbes. On account of the toxicity of nickel and its scarcity in the environment,

academic.oup.com
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BREAKING NEWS: The Royal Swedish Academy of Sciences has decided to award the 2022 #NobelPrize in Chemistry to Carolyn R. Bertozzi, Morten Meldal and K. Barry Sharpless “for the development of click chemistry and bioorthogonal chemistry.”
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Tn7 transposition depends on its AAA+ ATPase adaptor TnsC. A new #cryoEM structure from @alba_guarne & @shen_yao10 @mcgillu shows how TnsC targets DNA sequences between target & insertion sites to regulate sequence-specific transposon insertion. nature.com/articles/s41594-0…
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