@GuptaLab1

We make tools that render a quantitative view of #membraneproteins and #lipids at the #cellmembrane @YaleCellBio. #Massspectrometers are our favorite toys

 Yale School of Medicine
theguptalab.com/
Joined October 2021
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  • Following330
  • Followers342
8Media
Excited to see our work on the cover of @naturemethods We present a #nativemassspectrometry platform to detect #membraneprotein #lipid complexes directly from tunable #membranes Special thanks to Hannah Wang. One for #TeamMassSpec @YaleCellBio @YaleWestCampus @YaleMed @Yale
Nature Methods@naturemethods9 Jun 2023
Our June issue is live! 🎉 nature.com/nmeth/volumes/20/… On the cover, from @GuptaLab1 and artist Hanna Wang, a native mass spectrometry platform captures membrane protein–lipid organization directly from tunable lipid membranes. Paper here: nature.com/articles/s41592-0…
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If @ #BPS2025 , check out the talk by @CBcellbiologist today in the "Protein Lipid Interactions: Structure and Dynamics" Session @ 12PM. She will present our recently developed platform to capture membrane proteins in their native nanodomains & some exciting applications of it
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Very excited to see this work coming out. Combining membrane-active polymers with #proteomics we present the most optimal conditions to extract>2000 individual endogenous membrane proteins into nativenanodiscs @YaleCellBio @YaleWestCampus @YaleMed
Nature Methods@naturemethods2 Dec 2024
Work from @GuptaLab1 presents a high-throughput platform for extracting membrane proteins into native nanodiscs that retains the local membrane context, accompanied by a database of optimal extraction conditions for 2000+ mammalian membrane proteins. nature.com/articles/s41592-0…
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All Data publicly available via polymerscreen.yale.edu & polymerscreen.streamlit.app Searchable through Uniprot names. We hope this will enable #membraneprotein researchers to extract & study their favorite proteins, at endogenous expression levels, directly from native environment
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Work was led by @CBcellbiologist and @SnehasishG87, with critical contributions from other lab members & labs of  @moitrayee_lab @Yanshen73854711 & Rothmanlab
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If you are @ProteinSociety annual meeting, check out the talks and posters by @CBcellbiologist and @Rachelmcalli on our new tools to understand how the native #membrane regulates #membraneproteins and its application on a B cell protein (with @moitrayee_lab)
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If you are @ #BPS #Membrane #Biophysics meeting @Tahoe, check out Caroline's talk today. She describes our new & exciting platform to capture >2000 unique #membraneproteins in their native nanodomains with high efficiency and nanoscale spatial resolution
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If @ #ASMS2024, check out all our exciting works describing new technologies to study molecular organization of #membraneproteins and #lipids at the #membrane
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Meet us @moitrayee_lab at #BPS2024 with posters from @GTWalker7 and @Rachelmcalli and my talk at the #FutureofBiophysics Burroughs Wellcome Fund symposium. We will highlight our work on determining #membraneprotein #organization and #structure directly from native #membranes.
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If @ #BPS2024, check out all our exciting works describing new technologies to study molecular organization of #membraneproteins and #lipids directly from the #membrane and its applications in understanding various membrane-associated signaling events
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very happy to play our part in the fantastic work led by @moitrayee_lab, in collaboration with the @MuzumdarLab. Work spearheaded by two of our champion grad students @GTWalker7 and @CBcellbiologist
Bhattacharyya Lab@moitrayee_lab4 Dec 2023
Here is a tweetorial summarizing findings from our recent paper from @YalePharm @YaleMed in @NatureNano by @GTWalker7 and @CBcellbiologist. Dream team @shalgene @Xiangyu_GigiG @MuzumdarLab @GuptaLab1! Also see the N&Vs at nature.com/articles/s41565-0… by @KrishnanYamuna!
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A very exciting application of #ESIBD
Kelvin Anggara@vixklen12 Oct 2023
Out today in @ScienceMagazine: Seeing glycans attached to proteins and lipids - at the *single molecule level*! 🎉🎉🎉 doi.org/10.1126/science.adh3… #ScienceResearch #glycotime #teammassspec My 1st #tweetorial🧵(1/n) 👇👇👇
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What a cool tool!!
Yongli Zhang Lab at Yale@ZhanglabY13 Oct 2023
Our article on in vivo force sensors appeared in Science Advances. Thank Yuan Ren, Julien Berro @BerroLab, and Jie Yang! Force redistribution in clathrin-mediated endocytosis revealed by coiled-coil force sensors | Science Advances science.org/doi/full/10.1126…
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Extremely honored to be recognized as an Emerging Investigator by the American Society for Mass Spectrometry @asmsnews. Credit goes to my amazing lab members for their hard work. Special thanks to my past mentors & present colleagues at @YaleWestCampus @YaleCellBio @YaleMed
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Turns out Gas phase is not that bad! Very exciting
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See our lab's latest JASMS publication! In this work, our lab deploys this quantitative CIU methodology to measure isomass pairs of biotherapeutics. Find it here: pubs.acs.org/articlesonreque… #ASMS #JASMS #CIU #MassSpec #anitbodies
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Kallol Gupta retweeted
Melia Lab@TJ_MeliaLab15 Aug 2023
Congrats Justin! Really nice work showing ATG2 function on monolayer-wrapped LDs and careful characterization of a transport dead mutant which loses bridge-like but not shuttle-like lipid transport capacity. Thanks also to @Rachid_Thiam for cool studies with monolayer mimics.
Justin Korfhage@JLeonard_0115 Aug 2023
I’m excited to finally share this project from the @TJ_MeliaLab where we demonstrate that the lipid transport protein ATG2 is active and highly efficient on phospholipid monolayers. (1/3) biorxiv.org/content/10.1101/…

ATG2A-mediated bridge-like lipid transport regulates lipid droplet accumulation

ATG2 proteins facilitate bulk lipid transport between membranes. ATG2 is an essential autophagy protein, but ATG2 also localizes to lipid droplets (LDs), and genetic depletion of ATG2 increases LD...

biorxiv.org
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If you are @ProteinSociety meeting #PS37, please check out the exciting work of @anipan84 (Poster20AB), showing how we can directly determine macromolecular organization of #membraneproteins and #lipds directly from synthetic and cellular membranes using #nativemassspectrometry
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If you are @ProteinSociety meeting #PS37, please check out the exciting work of our grad student @Rachelmcalli (Poster114AB), showing how lipid vesicle #nativemassspectrometry can decipher membrane recruitment mechanism of a B-cell kinase
Bhattacharyya Lab@moitrayee_lab13 Jul 2023
Rachel McAlister @Rachelmcalli is representing us at the @ProteinSociety meeting #PS37. Stop by her poster 114AB to check out a native MS based general experimental platform to identify and quantify lipid binding in membrane association domains (collaboration with @GuptaLab1)
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Check out our new study published at @NatureComms in which we investigated the Akt1 #signaling and dynamics using an optogenetic-phosphoproteomic system, or Optop-DIA! nature.com/articles/s41467-0… @YalePharm @CancerYale@YaleWestCampus@yalemed 1/4
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