Scientific co-founder and head of research at Neptune Bio
New York, NY
Joined December 2013
- Tweets128
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harm retweeted
Excited to share VIPerturb-seq!
New tech from my lab which aims to improve the cost, data quality, and efficiency of single-cell CRISPR screens so that they are accessible to any lab - even at genome-wide scale
Preprint and 🧵 (1/): biorxiv.org/content/10.64898…
harm retweeted
Our next @cegs_ica virtual seminar will be on 5/2 at 12PM ET
@mh_kowalski and @harm__w will present 'CPA-Perturb-seq', extending the Perturb-seq framework to study cleavage and polyadenylation, a post-transcriptional regulatory process.
Free registration: nygenome.zoom.us/meeting/reg…
harm retweeted
We are excited to release Seurat v5- with new methods for multimodal, spatially resolved, and massively scalable single-cell analysis. satijalab.org/seurat
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We are excited to introduce Phospho-seq, our approach for profiling intracellular protein and cell signaling dynamics alongside additional molecular modalities in single cells!
Check out @JBlairSci's preprint at biorxiv.org/content/10.1101/…
harm retweeted
We've been working on Seurat v5 for two years and are excited to share it with you soon!
In a webinar w/@10xGenomics and @ScienceMagazine on 3/29, I'll introduce new methods to analyze spatial/multimodal datasets that scale to tens of millions of cells: view6.workcast.net/register?…
harm retweeted
New preprint w/@anshulkundaje introducing CPA-Perturb-seq! We systematically perturb regulators of cleavage and polyadenylation, and explore post-transcriptional changes at single-cell resolution. Led by @mh_kowalski @harm__w and @jjohlin (🧵)
biorxiv.org/content/10.1101/…
harm retweeted
Excited to share our preprint on multimodal single-cell sequencing to characterize antigen-specific CD8+ T cells across COVID vaccination and disease. We also release datasets profiling ATAC, RNA, protein, TCR and MHC dextramers at single-cell resolution:
biorxiv.org/content/early/20…
harm retweeted
Interested in single-cell CRISPR screens for combinatorial perturbations?
Check out CaRPool-seq, where multiple gRNA 'carpool' together on a self-cleavable array.
Now out in @naturemethods: nature.com/articles/s41592-0…
And request our free Starter Kit to try it yourself!
harm retweeted
Can we quantitatively tune endogenous gene expression to understand dose-dependent processes in cells?
@GemmaNoviello has developed a 🛠️ to do that - #CasTuner 📻 - with the help of @RutgerGjaltema
👉 biorxiv.org/content/10.1101/…
See Gemma‘s tweetorial 👇
harm retweeted
Congrats to lab postdocs @alexmenman & @harm__w who cloned today's @Addgene Plasmid of the Day (!) for RNA-targeting CRISPR screens:
sanjanalab.org/reprints/Wess…
🙌👨🔬✂️⚙️
pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Depositor: @nevillesanjana
Purpose: Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus
harm retweeted
Yay! We got an @theNCI award w/ @nevillesanjana to develop CRISPR systems that modulate splicing in vivo to better understand its role in disease. Postdoc positions available: nygenome-openhire.silkroad.c… Please RT!
harm retweeted
Check out two exciting talks at our next @cegs_ica virtual series!
'Bridge' integration: harmonizing single-cell data across modalities (@YUHANHAO2)
NTT-seq: measuring multiple histone mofifications in the same sample/cell (@timoast)
Free registration: nygenome.zoom.us/meeting/reg…
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A tweetorial about my postdoc work in collaboration with @Chrisilx @braingov @Zhisong_he @lucaspelkmans @GrayCampLab @TreutleinLab.
biorxiv.org/content/10.1101/…
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Check out NTT-seq for all your multimodal chromatin needs! an amazing collab with @raimondi_ivan @NYGCtech: biorxiv.org/content/10.1101/…
#singlecell and #chromatin folks!We are excited to introduce NTT-seq, a new technology that allows for multifactorial chromatin profiling both in bulk and at single-cell resolution!Check it out!
Another amazing collab piece by @NYGCtech and @satijalab at @nygenome
harm retweeted
Our new preprint, led by @YUHANHAO2, introduces bridge integration: cross-modality alignment via a multiomic 'bridge' dataset.
We map single-cell ATAC-seq, methylation, CUT&Tag and CyTOF profiles onto scRNA-seq references- scaling to millions of cells.
biorxiv.org/content/10.1101/…
harm retweeted
Our next @cegs_ica virtual seminar is on 2/22:
@harm__w will introduce CaRPool-seq- a single-cell screening technology using Cas13 to simultaneously perturb multiple genes, measure multimodal responses, and explore genetic interactions
Free registration: nygenome.zoom.us/meeting/reg…
harm retweeted
Great to see the Signac paper out in Nature Methods today! Check out the Signac package if you're analyzing scATAC-seq or scCUT&Tag data: github.com/timoast/signac
The Signac framework enables end-to-end analysis of single-cell chromatin data and offers interoperability with the Seurat package for multimodal analysis. @timoast @satijalab @nygenome nature.com/articles/s41592-0…
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Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells #NBThighlight by the @nevillesanjana lab
cell.com/cell-chemical-biolo…
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A boost for biomedical research/drug development: @nevillesanjana of @nygenome, @nyuniversity & @nyugrossman develops guides that optimize/extend RNA-targeting CRISPR in human cells: bit.ly/2VfRFPp @CellChemBiol @alexmenman @we_harm @Synthego @NEBiolabs