Molecular microbiologist intrigued in AMR💊, metabolism⚙️ and gene regulation🧬 of pseudomonads. Senior researcher at Dept. of Microbiology, Univ. de Sevilla
Braunschweig, Alemania
Joined February 2015
- Tweets829
- Following227
- Followers177
- Likes2.5K
Pinned Tweet
🚨 New paper out! Collaboration between @hausslerlab, @tuBraunschweig, and @PabloINik, @LabNikel
In this protocol, we describe a #CRISPR-Cas9 -Recombineering system for the genetic #engineering of #Pseudomonasaeruginosa lab strains and clinical/environmental isolates (🧵 1/7)
#NewNProt: New #CRISPRCas9 -assisted #recombineering tools allowing genetic editing of intractable
#Pseudomonas aeruginosa isolates bit.ly/3rJnqke @HausslerLab @Helmholtz_HZI @tuBraunschweig
Alejandro Arce R. retweeted
Our group is looking for a PostDoc with a strong background in molecular microbiology, strain design and genetic engineering. Join us at @Rigshospitalet and @DTUtweet to work on bacterial infections and #AMR. Please RT and apply!
tinyurl.com/3kt2yxmj
Alejandro Arce R. retweeted
Quadruplet codon decoding-based versatile genetic biocontainment system
academic.oup.com/nar/article…
Alejandro Arce R. retweeted
Fantástico Andony, has hecho un trabajo excelente de la mano de @aleyo_arce Mucha suerte en tu búsqueda de tesis doctoral y en nuestro lab siempre tendrás tu casa 👏🏼👏🏼👏🏼
Pues se acabó el máster ✨🙂↔️✨
Gracias a las instituciones y los grupos de investigación que han sido parte de mi formación académica (@Ibarra_Lab y @pbernal209 ) y por supuesto, a mi familia y amigos que me apoyan siempre 🫶🏻 vivan los microbios y la genética! 🧬🔬🦠
Alejandro Arce R. retweeted
The #Microbes for #sustainability workshop #Baeza @AytoBaeza continues w/a great example of #bacterial #biocontrol by @pbernal209, complete w/a full mechanistic explanation of how Type VI #secretion systems work in #Pseudomonas @UniversidadAnd
Alejandro Arce R. retweeted
Absolutely honoured to host Microbiology legend Ken Timmis @KennethTimmis at @unisevilla this week! His talk on International Microbiology Literacy Initiative was truly inspiring. Grateful for invaluable stories, advice & experiences shared. It was very special to have him here.
Alejandro Arce R. retweeted
Off to a good start in 2024! Thrilled to share the pAblo·pCasso toolset for unconstrained cytidine/adenine-base editing in #bacteria led by @KaterinaKozaeva w/Zach Nielsen & @ManuelNietoDom1 @LabNikel @DTUBiosustain🦠@NAR_Open #SynBio #OpenAccess #CRISPR
academic.oup.com/nar/advance…
Alejandro Arce R. retweeted
Revolutionizing gene libraries in E. coli! Cerdan et al present a new approach for efficient integration of large gene libraries into the bacterial chromosome to address protein toxicity & instability issues. A new functional screening in biotech! ➡️ ami-journals.onlinelibrary.w…
Alejandro Arce R. retweeted
#Environmental #Microbiology #workshop 2024 led by Juan Luis Ramos, @vdlorenzo_CNB & Puri López-García · Speakers include @pbernal209 @mSystemsJ @portokalica @KennethTimmis & others—applications open till Jan 15th @AMIposts #Baeza #Spain #biotechnology
Former scientific colleagues, now friends for a life time 🫶🏻
Happy gathering of past, present & future scientific luminaries for joining the memorable celebration & wedding party of our dear friends and colleagues @Alberto_SPJ & @Mery_PipiS 👇
1
1
177
Alejandro Arce R. retweeted
Martes 21 de Noviembre a las 22:45 La Pandemia Silenciosa, India. En @laSextaTV
@CarlosMTBVAC @unicomplutense @SEMicrobiologia @SEIMC_ @microBIOblog @CSIC @AruUcm @PRANgob @jalisdelaserna @EnviadoTV @opsoms @vacunando @esmisionposible @animalshealthes @isanidad
Alejandro Arce R. retweeted
Every Xmas season the @CNB_CSIC organizes a 1-day workshop for expat Spanish postdocs & young PIs abroad willing to share what they are doing and widen their network of local colleagues. Also, a chance to survey the domestic job market 😅. Please email organizers if interested 👇
The availability of different AbR cassettes enables also genetic engineering of #clinicalisolates. We include also a whole section with guidelines and recommendations that facilitate the modification of these intractable strains (7/7)
59
Both vector sets are available with several antibiotic resistance cassettes, which allows iterative engineering of different alleles without the need for plasmid curing. The complete toolkit can be purchased at @Addgene addgene.org/browse/article/2… (6/7)
Overall, the minimum time required to carry out a desired genomic modification is 5 working days, having already prepared all the materials required for the genetic engineering of a given lab, clinical or environmental strain (5/7)
77
The mutagenesis oligonucleotide requires only 40-50 nt homology regions in order to introduce virtually any type of modification, including short and long deletions, short insertions, and SNPs with varying efficiencies (4/7)
73
The second plasmid harnesses the inducible expression of a gene coding the powerful #Pputida Ssr recombinase described recently by @vdlorenzo_CNB. This protein promotes the recombination of a ssDNA mutagenesis oligonucleotide that introduces the desired mutation (3/7)
1
74
Alejandro Arce R. retweeted
#NewNProt: New #CRISPRCas9 -assisted #recombineering tools allowing genetic editing of intractable
#Pseudomonas aeruginosa isolates bit.ly/3rJnqke @HausslerLab @Helmholtz_HZI @tuBraunschweig
Alejandro Arce R. retweeted
😳Genome-wide screening reveals metabolic regulation of stop-codon readthrough by cyclic AMP:
academic.oup.com/nar/article…