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Open Notebook Science

@ONScience

Updates from #openscience notebooks. Start yours at https://xitter.cf/t.co/oeSw8NaDSL ...#opennotebookscience and #openlabnotebooks brought to you by @openscience

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Joined October 2013
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Open Notebook Science retweeted
genefish (@genefish) profile picture
genefish@genefish15 Dec 2019
New Notebook Post: Laura’s Notebook: QuantSeq Purification and qPCR Assay Batches 1 & 2 ift.tt/36DCPlE
Laura’s Notebook: QuantSeq Purification and qPCR Assay Batches 1 & 2

Laura’s Notebook: QuantSeq Purification and qPCR Assay Batches 1 & 2

ds cDNA purification, pre-PCR I purified the ds cDNA from batches 1 and 2. Notes on how to improve that process: Bubbles! Bubbles make it difficult to use a mutichannel pipette. Need to improve han…

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genefish@genefish14 Dec 2019
New Notebook Post: Grace’s Notebook: Attempt at Concentrating Pooled (made from extra extracted RNA) Samples with the Zymo Microprep kit ift.tt/38Equzu
Grace’s Notebook: Attempt at Concentrating Pooled (made from extra extracted RNA) Samples with the...

Grace’s Notebook: Attempt at Concentrating Pooled (made from extra extracted RNA) Samples with the...

So, it turns out my 6 pooled samples aren’t concentrated enough for NWGC to sequence. They are all at around 25ng/ul, but they need to be at least 50ng/ul. I called zymo yesterday to find out how b…

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SGC Open Lab Notebooks@SGClabNotebooks9 Dec 2019
Magda Szewczyk @thesgconline tests a kit to identify novel mono-methylated substrates of the methyltransferase PRMT7. Blog: bit.ly/2RwZbkJ Report: bit.ly/2RDpxl1
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Open Notebook Science retweeted
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SGC Open Lab Notebooks@SGClabNotebooks6 Dec 2019
Claudia Alvarez @thesgconline purifies and starts to characterize biophysically the C-HEAT domain of the #huntingtonsdisease protein HTT. #Openscience report: bit.ly/33Xztse Blog: bit.ly/2Pp4xf0 @HuntingtonSC @HDSA @HDBuzzFeed @HDAwareness
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Open Notebook Science retweeted
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genefish@genefish7 Dec 2019
New Notebook Post: Laura’s Notebook: QuantSeq Library Generation Batch 2 ift.tt/36hVAex
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genefish@genefish7 Dec 2019
New Notebook Post: Yaamini’s Notebook: December 2019 Goals ift.tt/2PoEeWw
Yaamini’s Notebook: December 2019 Goals

Yaamini’s Notebook: December 2019 Goals

As usual Dwight K. Schrute is right. The end of the quarter is here and I’ve got a few things I want to check off my to-do list before the decade ends. November Goals Recap Virginica Gonad Methylat…

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Open Notebook Science retweeted
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genefish@genefish6 Dec 2019
New Notebook Post: Laura’s Notebook: QuantSeq Library Generation Batch 1 ift.tt/340a5SM
Laura’s Notebook: QuantSeq Library Generation Batch 1

Laura’s Notebook: QuantSeq Library Generation Batch 1

Began my full QuantSeq library prep today. I am processing ctenidia samples first, and since there are 53 samples I’m doing ~half at a time. Today I generated double stranded cDNA for 26 samples + …

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genefish@genefish3 Dec 2019
New Notebook Post: Grace’s Notebook: December 2019 Goals ift.tt/2DJQqvC
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Rachel Harding (@LabScribbles) profile picture
Rachel Harding@LabScribbles4 Dec 2019
My latest #openlabnotebook updates on all that I have been up to in the lab of late #openscience #opendata #huntingtonsdisease labscribbles.com/2019/12/03/…
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genefish@genefish27 Nov 2019
New Notebook Post: Sam’s Notebook: qPCR – Geoduck hemolymph and hemocyte cDNA with vitellogenin primers ift.tt/37JHMuH
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genefish@genefish24 Nov 2019
New Notebook Post: Yaamini’s Notebook: DML Analysis Part 43 ift.tt/2DcAh1q
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genefish@genefish23 Nov 2019
New Notebook Post: Grace’s Notebook: Pooled 6 new samples for sequencing ift.tt/37ETxmi
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The Neuro@TheNeuro_MNI22 Nov 2019
Mahdieh Tabatabaei is aware of the of the privilege and responsibility her #OpenScience work entails: 'It provides hope for patients, and gives researchers a sense that they are making a difference more than they ever could.” ℹ️ at.theneuro.ca/OSPlatformsCo… #OpenScienceinAction
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Open Notebook Science retweeted
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genefish@genefish21 Nov 2019
New Notebook Post: Laura’s Notebook: Oly methylation analysis, Nov 20, 2019 ift.tt/2QBbQCz
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Victoria Vu @thesgconline tries hard and eventually succeeds in over-expressing HTT, the protein associated with #huntington’s disease, in neuronal cells. Blog: bit.ly/2NZKmVA Methods: bit.ly/2CVgTpx @HuntingtonSC @HDSA @HDBuzzFeed @HDAwareness
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anneclinio@anneclinio18 Nov 2019
Nice listen to @ReaderMeter explain @cziscience software funding. I was wondering if they are funding any open lab notebooks initiative. Data management relies on documentation. It is a foundational tool to maximizes impact and quality. #OpenScienceinAction @TheNeuro_MNI
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Open Notebook Science retweeted
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genefish@genefish15 Nov 2019
New Notebook Post: Grace’s Notebook: More RNA extracted from Day 9 – RNA in all ift.tt/2NPuPYu
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Mandeep Mann @thesgconline uses virtual screening to significantly increase the potency of her USP5 ligands. #Openscience report: bit.ly/35a3g1V Blog: bit.ly/32W5i4h
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Ashley Hutchinson @thesgconline starts her open lab notebook and presents her project where she will analyze the 3D structure of proteins involved in amyotrophic lateral sclerosis (#ALS). Welcome, Ashley! bit.ly/2ObFk7f @alsassociation @iamalsorg @ALSTDI
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Being open is also about embracing failures. Open your lab notebooks! Great talks by @LabScribbles and @ThomasDurcan #EmbraceTheFail #OpenScienceinAction @TheNeuro_MNI
Image from Adrien Peyrache 🪐😴⚡️ (@apeyrache)
Image from Adrien Peyrache 🪐😴⚡️ (@apeyrache)
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