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Open Notebook Science

@ONScience

Updates from #openscience notebooks. Start yours at https://xitter.cf/t.co/oeSw8NaDSL ...#opennotebookscience and #openlabnotebooks brought to you by @openscience

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Joined October 2013
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Really impressed with the open notebooks being kept by @sr320's @EIMD_fhl students onsnetwork.org/eimd/ #openscience as a matter of course
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OSM: Synthesis of 4-iodopyrimethamine analogue (BC_2020_05) bit.ly/3dG7ALG
OSM: Synthesis of 2-(4-iodophenyl)-3-(2-methylpropoxy)-pent-2-enenitrile (BC_2020_02) bit.ly/34dElwx
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OSM: Synthesis of 2-(4-iodophenyl)-3-oxopentanenitrile (BC_2020_01) bit.ly/2TfTh6Z
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genefish@genefish15 Dec 2019
New Notebook Post: Laura’s Notebook: QuantSeq Purification and qPCR Assay Batches 1 & 2 ift.tt/36DCPlE
Laura’s Notebook: QuantSeq Purification and qPCR Assay Batches 1 & 2

Laura’s Notebook: QuantSeq Purification and qPCR Assay Batches 1 & 2

ds cDNA purification, pre-PCR I purified the ds cDNA from batches 1 and 2. Notes on how to improve that process: Bubbles! Bubbles make it difficult to use a mutichannel pipette. Need to improve han…

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genefish@genefish14 Dec 2019
New Notebook Post: Grace’s Notebook: Attempt at Concentrating Pooled (made from extra extracted RNA) Samples with the Zymo Microprep kit ift.tt/38Equzu
Grace’s Notebook: Attempt at Concentrating Pooled (made from extra extracted RNA) Samples with the...

Grace’s Notebook: Attempt at Concentrating Pooled (made from extra extracted RNA) Samples with the...

So, it turns out my 6 pooled samples aren’t concentrated enough for NWGC to sequence. They are all at around 25ng/ul, but they need to be at least 50ng/ul. I called zymo yesterday to find out how b…

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SGC Open Lab Notebooks@SGClabNotebooks9 Dec 2019
Magda Szewczyk @thesgconline tests a kit to identify novel mono-methylated substrates of the methyltransferase PRMT7. Blog: bit.ly/2RwZbkJ Report: bit.ly/2RDpxl1
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SGC Open Lab Notebooks@SGClabNotebooks6 Dec 2019
Claudia Alvarez @thesgconline purifies and starts to characterize biophysically the C-HEAT domain of the #huntingtonsdisease protein HTT. #Openscience report: bit.ly/33Xztse Blog: bit.ly/2Pp4xf0 @HuntingtonSC @HDSA @HDBuzzFeed @HDAwareness
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Open Notebook Science retweeted
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genefish@genefish7 Dec 2019
New Notebook Post: Laura’s Notebook: QuantSeq Library Generation Batch 2 ift.tt/36hVAex
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genefish@genefish7 Dec 2019
New Notebook Post: Yaamini’s Notebook: December 2019 Goals ift.tt/2PoEeWw
Yaamini’s Notebook: December 2019 Goals

Yaamini’s Notebook: December 2019 Goals

As usual Dwight K. Schrute is right. The end of the quarter is here and I’ve got a few things I want to check off my to-do list before the decade ends. November Goals Recap Virginica Gonad Methylat…

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Open Notebook Science retweeted
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genefish@genefish6 Dec 2019
New Notebook Post: Laura’s Notebook: QuantSeq Library Generation Batch 1 ift.tt/340a5SM
Laura’s Notebook: QuantSeq Library Generation Batch 1

Laura’s Notebook: QuantSeq Library Generation Batch 1

Began my full QuantSeq library prep today. I am processing ctenidia samples first, and since there are 53 samples I’m doing ~half at a time. Today I generated double stranded cDNA for 26 samples + …

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genefish@genefish3 Dec 2019
New Notebook Post: Grace’s Notebook: December 2019 Goals ift.tt/2DJQqvC
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Open Notebook Science retweeted
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Rachel Harding@LabScribbles4 Dec 2019
My latest #openlabnotebook updates on all that I have been up to in the lab of late #openscience #opendata #huntingtonsdisease labscribbles.com/2019/12/03/…
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QualitativeUAEM: Grupos de enfoque, grupos de discusión bit.ly/37UoW4b
QualitativeUAEM: ¿Conoces algo de la recolección de datos en la investigación cualitativa?, ¿no? Aquí te contamos… bit.ly/2OVclFg
QualitativeUAEM: Al desarrollar una técnica de análisis como la “Historia de Vida”, todo cuidado es poco bit.ly/2L5NDko
QualitativeUAEM: Técnicas de investigación cualitativa y cuantitativa, ¿hay alguna mejor? bit.ly/2r16o1B
QualitativeUAEM: Lengua y habla, ¿los conectas? bit.ly/35J9ZA2
QualitativeUAEM: ¿Sabías que si no proteges tu trabajo podrían robártelo? Si no lo sabes, tal vez deberías de leer esto bit.ly/2L6txXn
QualitativeUAEM: ¿Cuántos tipos de conocimiento puedes identificar? bit.ly/2L5pgDf
QualitativeUAEM: De la Etnografía “aburrida”, a la Etnografía de Nigel Barley bit.ly/2DuSsj2
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