@LabParp

Research in biochemistry and structural biology of PARP enzymes and bacterial toxins

Joined January 2021
  • Tweets42
  • Following63
  • Followers102
2Media
Excited to have been awarded @lunduniversity infrastructure support to establish a mini structural bioinformatics facility, LU-Fold. Watch this space!
3
3
1
60
PARP lab Lund retweeted
Eugene Kim@eugene_kim__14 May 2022
Excited to share new preprint from my lab @MPIbp in collaboration with #CamillaBjörkegren’s lab at Karolinska Institute! biorxiv.org/cgi/content/shor… (0/n) In this work, we reveal that Smc5/6 complex is a DNA loop extruding motor.
12
62
7
321
Smc5/6 extrudes DNA loops, great important work by Camilla Björkegren and @eugene_kim__ doi.org/10.1101/2022.05.13.4…
1
The Smc5/6 complex is a DNA loop extruding motor biorxiv.org/content/10.1101/…

The Smc5/6 complex is a DNA loop extruding motor

Structural Maintenance of Chromosomes (SMC) protein complexes are essential for the spatial organization of chromosomes. While cohesin and condensin organize chromosomes by extruding DNA loops, the...

biorxiv.org
1
3
PARP lab Lund retweeted
hopfnerlab@hopfnerlab18 Jan 2022
Another step towards uncovering the secrets of the Schlafen protein family. Check out how we tackled SLFN5 by crystallization and #CryoEM : academic.oup.com/nar/advance… – at Munich, Germany
10
2
42
Forskningsingenjör i Cryo-EM at @lunduniversity @medfak_LU lu.varbi.com/en/what:job/job…

Research engineer to the cryo-EM research community

Cryo-EM at Lund U niversit y A growing group of researchers at Lund University are using cryo-electron microscopy, especially in its "single-particle" form, to investigate their structural biological

lu.varbi.com
1
1
1
2 year Cryo-EM engineer job at Lund University. Don't be put off that the ad is in Swedish, as the only formal language requirement is communication skills in English
Vasili Hauryliuk@hauryliuk18 Jan 2022
Forskningsingenjör i Cryo-EM at @lunduniversity @medfak_LU lu.varbi.com/en/what:job/job…

Research engineer to the cryo-EM research community

Cryo-EM at Lund U niversit y A growing group of researchers at Lund University are using cryo-electron microscopy, especially in its "single-particle" form, to investigate their structural biological

lu.varbi.com
6
5
PARP lab Lund retweeted
John Pascal Lab@LabPascal29 Nov 2021
Happy to share our first Cryo-EM structure! Cryo-EM structures and biochemical insights into heterotrimeric PCNA regulation of DNA ligase cell.com/structure/fulltext/…

Cryo-EM structures and biochemical insights into heterotrimeric PCNA regulation of DNA ligase

Sverzhinsky et al. use cryoelectron microscopy to investigate the complex formed by DNA ligase and the sliding clamp PCNA as they bind a nicked DNA molecule. They obtain snapshots of DNA ligation...

cell.com
2
17
75
We have our LEX system in place. So nice! Highly recommended for any lab doing overexpression in E.coli. Thanks everyone at @epiphyte3 for great engineering, help with shipping, and a foolproof installation manual. – at Lund, Sverige
1
7
Sorry we forgot to add the time: it will be 5pm Paris, 11am Boston, 11pm Beijing like for the first series.🧐 In case, the poster updated with the time is added to this post.👍
6
20
Congrats Marion, Ivan and everyone at @IvanAhelLab, great study on DarT published in Nature : nature.com/articles/s41586-0…
4
1
24
Awesome, congrats!
Vasili Hauryliuk@hauryliuk19 Aug 2021
Happy to see it finally out in @MolecularCell Mohammad Roghanian et al.: (p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains. This project took at least 4 years... sciencedirect.com/science/ar…
1
2
PARP lab Lund retweeted
Eric Brown
@ericmichbrown16 Aug 2021
Super excited to be able to share this study on gut microbiome ADP-ribosyltransferases and their role as phage-encoded fitness factors in the gut. Been a great collaboration with @HASCiencia and @Novartis along with @broadinstitute @MGH_RI & @emilytemple_ cell.com/cell-host-microbe/f…

Gut microbiome ADP-ribosyltransferases are widespread phage-encoded fitness factors

Brown et al. report prevalent ADP-ribosyltransferases in gut microbiome commensals that are associated with bacteriophage elements and secreted as active enzymes. An abundant ADP-ribosyltransferase...

cell.com
12
37
2
136
The expression plasmid for our ADP-ribosylation detector protein is available from @Addgene: addgene.org/160665/

pNIC28-MacroGreen

Plasmid pNIC28-MacroGreen from Dr. Herwig Schüler's lab contains the insert Af1521 modified synthetic construct eGFP fusion and is published in Commun Biol. 2021 Jul 28;4(1):919. doi: 10.1038/s4200...

addgene.org
Communications Biology@CommsBio29 Jul 2021
García Saura et al. developed a simple research tool for detecting ADP-ribosylation by GFP fluroescence. Macrogreen is produced by expression in E. coli and can reveal mono-and poly-ADP-ribosylation of diverse proteins in vitro. @LabParp @ubiquitin_neuro nature.com/articles/s42003-0…
2
4
16
Not too late to apply for a funded position as postdoc in our lab, a collaboration with the great @gem__atkinson, the great @hauryliuk and the great @watz_wolf
PARP lab Lund@LabParp7 Jul 2021
Replying to @LabParp
Please spread the word about our funded postdoctoral position (apply before August 15): jiscmail.ac.uk/cgi-bin/wa-ji…
4
1
4
Not just for the PARP community: our new tool for detecting ADPr on protein. Postdoc Antonio Ginés Garćia-Saura’s work with big thanks to @ubiquitin_neuro
Communications Biology@CommsBio29 Jul 2021
García Saura et al. developed a simple research tool for detecting ADP-ribosylation by GFP fluroescence. Macrogreen is produced by expression in E. coli and can reveal mono-and poly-ADP-ribosylation of diverse proteins in vitro. @LabParp @ubiquitin_neuro nature.com/articles/s42003-0…
3
1
12
PARP lab Lund retweeted
Da-Neng Wang@danengw28 Jul 2021
Hardcopies of the #CryoEM book by Bob Glaeser, @NogalesLab & @WahChiu1 I ordered months ago have finally arrived. Thanks to the editors and all the chapter authors, we now have an updated textbook for graduate level teaching.
1
4
1
54
Serine ADP-ribosylation marks nucleosomes for ALC1-dependent chromatin remodeling dlvr.it/S3Hlmz
1
2
2-year postdoc stipend available to work on bacterial toxin-antitoxin systems (structure and biochemistry) in our new lab at Lund University. Email me for details: herwig.schuler@biochemistry.lu.se
1
5
4
Please spread the word about our funded postdoctoral position (apply before August 15): jiscmail.ac.uk/cgi-bin/wa-ji…
1
1
Load more