We uncover how cells sense, communicate, and exchange material, with a focus on pH sensing, intercellular communication, and the dark proteome.
Miami
Joined August 2019
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PAM scanning, a CRISPR-based technique, maps where proteins tolerate insertions by testing function in living cells, and scales to any gene expressible in yeast. Out in JBC: 91 yeast GPCR chimeras, sites transferred to human A2A and MTNR1A receptors.
doi.org/10.1016/j.jbc.2026.1…
Our lab develops accurate, affordable, scalable platforms, including PAM scanning, DCyFIR, and PIONEER, to test protein function in living cells. They exemplify potential solutions to the experimental validation bottleneck as AI generates protein designs at enormous scale.
Congratulations to Jacinda Pujols, Kyutae Lee, Nick Kapolka, Sam Taylor, and Bruno Colon. Thank you to @NIGMS, the @Pap_Corps , and @SylvesterCancer for supporting this work.
Software: conda install -c bioconda pam_scanning
Thank you for sharing our work and for this thoughtful perspective on how science moves forward. It means a great deal to our team. We’re excited to see what others discover about these “superdark” proteins. Many questions still to explore!
A reminder on how science works. Hypotheses, evidence, analysis, results, peer scrutiny, alternative interpretations. Rinse, repeat.
Here's a snippet of that: a recent paper on so-called 'superdark' proteins and commentary from former Lister Fellow @m_madan_babu and @nilcm9 👇
IsomLab retweeted
Brain cancers take advantage of neural circuit activity. Re-purposing an anti-seizure med called levetiracetam disrupts an important neuron-cancer interaction in #DMG #DIPG, slowing growth and extending survival in mice and retrospective patient data.
nature.com/articles/s41591-0…
IsomLab retweeted
Happy to share our preprint: "A Unified 3D Generative Model for Synthesizable Structure-Based Drug Design". Highlights, preprint, and code below: 🧵1/10
IsomLab retweeted
67 years ago today, ‘Take Five’ by Dave Brubeck Quartet was released as a single.
It went on to become the best-selling jazz single of the 20th century.
IsomLab retweeted
BindCraft2 from the @MartinPacesa lab is here and available now on Ariax!
We called BindCraft a “DeepSeek moment” in protein design. BC2 builds on that proven foundation with major speed improvements and a much broader range of therapeutic formats.
ariax.bio/resources/bindcraf…
Snuck onto the cover of Nature. Not a bad place for a “superdark” protein to end up. Very grateful. Much deserved Kyutae Lee, Sam Taylor, PhD, Jennifer Arcuri, Ph.D., Shraddha ChandThakuri, Bruno Colon, Jacinda Pujols, and others.
Thank you, Nil and @m_madan_babu, for your cool commentary on our recent Nature paper. Very well done!
The paper: nature.com/articles/s41586-0…
The commentary: ‘Superdark’ protein looks like a G-protein-coupled receptor — but shows unconventional behaviour nature.com/articles/d41586-0…
Out online today in @Nature. The known protein universe holds hundreds of millions of members, yet most remain unexplored, a vast biological darkness that may hold important clues to human health and disease. We went looking in it.
doi.org/10.1038/s41586-026-1…
Supported by an NIH R35 award from @NIGMS, @SylvesterCancer, and the @Pap_Corps, through my endowed chair. Thanks also to Steven Dwoskin, whose recent gift supports what comes next.