Annie Campbell (@annie_campb) header
Annie Campbell (@annie_campb) profile picture

Annie Campbell

@annie_campb

PhD candidate in the @GreenLabJHMI studying bacterial translation

Joined May 2020
  • Tweets67
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  • Followers150
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Photo by Annie Campbell (@annie_campb)Photo by Annie Campbell (@annie_campb)Photo by Annie Campbell (@annie_campb)Photo by Annie Campbell (@annie_campb)Photo by Annie Campbell (@annie_campb)
Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI12 Sep 2024
We’ve captured the splitting image of Slh1: our work on an enzyme that splits collided ribosomes in E. coli is out on bioRxiv! Huge thanks to the @BeckmannLab, Burroughs, and Aravind for a great collaboration. biorxiv.org/content/10.1101/…
The RNA helicase HrpA rescues collided ribosomes in E. coli

The RNA helicase HrpA rescues collided ribosomes in E. coli

Although many antibiotics inhibit bacterial ribosomes, loss of known factors that rescue stalled ribosomes does not lead to robust antibiotic sensitivity in E. coli , suggesting the existence of...

biorxiv.org
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Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI14 Dec 2023
It’s official! MutS2 still doesn’t cut it. Congrats @Esth3rPark and @BeckmannLab! embopress.org/doi/full/10.10…
Green Lab (@GreenLabJHMI) profile pictureGreen Lab@GreenLabJHMI7 May 2023
Turns out MutS2 just doesn’t cut it ✂️ despite it having an SMR domain! Our latest collaboration with the @BeckmannLab characterizing the role of MutS2 in splitting stalled ribosomes in B. subtilis. biorxiv.org/content/10.1101/…
B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage

B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage

Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli , these pathways are triggered by ribosome collisions through...

biorxiv.org
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Annie Campbell retweeted
Heather Feaga (@FeagaHeather) profile picture
Heather Feaga@FeagaHeather5 Aug 2023
Lab’s first pre-print! We found that loss of EF-P and the ABCF-family ATPase YfmR is lethal to B. subtilis due to translation failures during early elongation. Great work Hye-Rim Hong, @cassidyprints and @letian_wu! #ribosomes biorxiv.org/content/10.1101/…
Identification of factors that prevent ribosome stalling during early elongation

Identification of factors that prevent ribosome stalling during early elongation

Protein synthesis is catalyzed by the ribosome and a host of highly conserved elongation factors. Most elongation factors that are conserved in all domains of life are essential, such as EF-Tu...

biorxiv.org
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Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI7 Jun 2023
The Green lab is looking to hire a postdoc to study ribosome stalling and rescue in bacteria! See our official posting (link below) for details. greenlabjhmi.org/new-page
Join Our Team! — The Green Lab @ JHMI

Join Our Team! — The Green Lab @ JHMI

greenlabjhmi.org
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Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI7 May 2023
Turns out MutS2 just doesn’t cut it ✂️ despite it having an SMR domain! Our latest collaboration with the @BeckmannLab characterizing the role of MutS2 in splitting stalled ribosomes in B. subtilis. biorxiv.org/content/10.1101/…
B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage

B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage

Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli , these pathways are triggered by ribosome collisions through...

biorxiv.org
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Annie Campbell retweeted
bioRxiv (@biorxivpreprint) profile picture
bioRxiv@biorxivpreprint7 May 2023
B. subtilis MutS2 splits stalled ribosomesinto subunits without mRNA cleavage biorxiv.org/cgi/content/shor… #bioRxiv
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Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI22 Feb 2023
Green lab gals get great grub and galavant for Galentine’s
Image from Green Lab (@GreenLabJHMI)
Image from Green Lab (@GreenLabJHMI)
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Excited to share our preprint on a crazy new anti-CRISPR mechanism: translation-dependent mRNA downregulation! biorxiv.org/content/10.1101/… Special thanks to all co-authors: Alexander Talaie, Heloise Carion, Yang Zhang, Sukrit Silas, @liyuping927 and @joeBondyDenomy!
Translation-dependent downregulation of Cas12a mRNA by an anti-CRISPR protein

Translation-dependent downregulation of Cas12a mRNA by an anti-CRISPR protein

Bacterial CRISPR-Cas systems cleave the genomic DNA of bacteriophage and other mobile genetic elements. Many temperate phages encode anti-CRISPR (Acr) proteins that use diverse and innovative...

biorxiv.org
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Annie Campbell retweeted
WilsonLab (@WilsonLab2) profile picture
WilsonLab@WilsonLab212 Nov 2022
Our paper is out on the Structure of Escherichia coli heat shock protein Hsp15 in complex with the ribosomal 50S subunit bearing peptidyl-tRNA…great work from ⁦⁦@HaarisAhsan⁩ and collaborators ⁦@IgnatovaLab⁩ and Tenson lab academic.oup.com/nar/advance…
Structure of Escherichia coli heat shock protein Hsp15 in complex with the ribosomal 50S subunit ...

Structure of Escherichia coli heat shock protein Hsp15 in complex with the ribosomal 50S subunit ...

Abstract. In Escherichia coli, the heat shock protein 15 (Hsp15) is part of the cellular response to elevated temperature. Hsp15 interacts with peptidyl-tR

academic.oup.com
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Annie Campbell retweeted
Bin Wu Lab (@binwu1) profile picture
Bin Wu Lab@binwu19 Nov 2022
Excited to share our preprint about "Bursting Translation on Single mRNAs in Live Cells" biorxiv.org/content/10.1101/… by Nathan Livingston @nliving93 and Jiwoong Kwon, in collaboration with @JianLiu_Lab, @GreenLabJHMI and @taekjip
Image from Bin Wu Lab (@binwu1)
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Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI28 Sep 2022
Congratulations to our awesome postdoc Franny Diehl for receiving this fellowship!
This Post is from an account that no longer exists. Learn more
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Annie Campbell retweeted
bioRxiv (@biorxivpreprint) profile picture
bioRxiv@biorxivpreprint28 Sep 2022
Transcription factors interact with RNA to regulate genes biorxiv.org/cgi/content/shor… #bioRxiv
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Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI9 Sep 2022
Group pic by the polysome at translational control 2022. Ribosome is life! #cshltranscon
Image from Green Lab (@GreenLabJHMI)
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Annie Campbell retweeted
EIRNABio (@EIRNABio) profile picture
EIRNABio@EIRNABio5 Aug 2022
Veltri et al. demonstrate that Hel2, Syh1, and Smy2, - proteins known to be involved in mRNA decay following ribosome stalling - act through distinct pathways. Syh1/Smy2 appear to act in a redundancy role to elicit no-go decay, upon Hel2 impairment pubmed.ncbi.nlm.nih.gov/3589…
Distinct elongation stalls during translation are linked with distinct pathways for mRNA degradat...

Distinct elongation stalls during translation are linked with distinct pathways for mRNA degradat...

Key protein adapters couple translation to mRNA decay on specific classes of problematic mRNAs in eukaryotes. Slow decoding on non-optimal codons leads to codon-optimality-mediated decay (COMD) and...

pubmed.ncbi.nlm.nih.gov
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😊🎉
Green Lab (@GreenLabJHMI) profile pictureGreen Lab@GreenLabJHMI1 Jul 2022
Congrats to our phenomenal grad student @annie_campb for being awarded an F31 from the NIAID! 🎉
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Annie Campbell retweeted
Beckmann Lab (@BeckmannLab) profile picture
Beckmann Lab@BeckmannLab20 Apr 2022
Pull the ripcord to clear the traffic jam: RQT splits collided #ribosomes by engaging the emerging mRNA. Check out our new story at #bioRxiv: biorxiv.org/content/10.1101/…
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Annie Campbell retweeted
Beckmann Lab (@BeckmannLab) profile picture
Beckmann Lab@BeckmannLab10 Mar 2022
Universal collisions!💥 Bacterial ribosome emergency ➡️ collision ➡️ disomes formation ➡️ recognition by SmrB ➡️ mRNA cleavage ➡️ ribosome rescue 🚑 Read the latest version of this story @Nature: nature.com/articles/s41586-0… Congrats to all people involved @GreenLabJHMI & A. Buskirk
Image from Beckmann Lab (@BeckmannLab)
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Annie Campbell retweeted
HHMI (@hhmi_science) profile picture
HHMI
@hhmi_science9 Mar 2022
HHMI News: Tiny cellular machines called ribosomes build proteins. When this building process goes awry in bacteria, ribosomes collide, triggering the arrival of a first responder molecule that begins a rescue operation. bit.ly/3vRGkF0
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Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI9 Mar 2022
The cutting edge: in bacteria, ribosome stalling leads to collisions that recruit SmrB, cleaving mRNA and initiating ribosome rescue. Great collaboration with the amazing @BeckmannLab! Read the published version here: nature.com/articles/s41586-0…
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Annie Campbell retweeted
Green Lab (@GreenLabJHMI) profile picture
Green Lab@GreenLabJHMI8 Mar 2022
Lab bowling to say goodbye and good luck to @anthonyveltri in his move to Wisconsin 😢 Best of luck - we’ll miss you!
Image from Green Lab (@GreenLabJHMI)
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