@SAGA_Complex

Multiprotein complex. About 1.6 MDa and 28x20 nm. Conserved in eukaryotes. Multifunctional. Transcriptional regulator.

 Eukaryotes
Joined October 2018
  • Tweets93
  • Following7
  • Followers166
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In this Review, Soffers and Workman discuss the initial discovery of the canonical SAGA complex, the subsequent studies that have shaped our view, and the latest structural work that provides insights into its function. @jerryleeworkman @ScienceStowers genesdev.cshlp.org/content/3…
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SAGA_COMPLEX retweeted
MED_COMPLEX@MED_COMPLEX17 Jul 2020
Nucleolar Pol II !!!! nature.com/articles/s41586-0…

Nucleolar RNA polymerase II drives ribosome biogenesis

Nature - RNA polymerase II has an unexpected function in the nucleolus, helping to drive the expression of ribosomal RNA and to protect nucleolar structure through a mechanism involving triplex...

nature.com
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SAGA_COMPLEX retweeted
AdelmanLab@AdelmanLab12 Feb 2020
How fast can the mammalian spliceosome act? Kirsten Reimer from the Neugebauer lab and Claudia Mimoso from my lab use millions of long read sequences, coupled with PROseq, to find out. The answer: super fast, often within 150-300nt of the splice junction. biorxiv.org/content/10.1101/…

Rapid and Efficient Co-Transcriptional Splicing Enhances Mammalian Gene Expression

Pre-mRNA splicing is tightly coordinated with transcription in yeasts, and introns can be removed soon after they emerge from RNA polymerase II (Pol II). To determine if splicing is similarly rapid...

biorxiv.org
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Replying to @domhelmlinger @SAGA_Complex @IGBMC
Can't stop watching this animation. Towards the end, the contacts between Tra1 (brown) and Spt20 (pink) are the regions that we identified and characterized biochemically and functionally in our latest paper in @NatureComms nature.com/articles/s41467-0…
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Our structure of the chromatin-modifying coactivator SAGA elucidates early events before transcription pre-initiation complex formation. Note related work from the Schultz lab published back-to-back. @mpibpc @igbmc nature.com/articles/s41586-0…
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TBP binding Cryo-EM revealing The SAGA structure #ChromatinHaiku nature.com/articles/s41586-0… nature.com/articles/s41586-0…
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Tra1 assembly Into NuA4, SAGA Via chaperones #ChromatinHaiku nature.com/articles/s41467-0…
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Glad to announce that our @biorxivpreprint is now published at @NatureComms ! nature.com/articles/s41467-0… We report some principles governing the assembly of multimeric transcriptional complexes, @SAGA and NuA4. Thread 1/7

Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in...

Nature Communications - Transcription initiation involves the coordinated assembly and activity of large multimeric complexes. Here the authors report on the chaperone-mediated ordered assembly of...

nature.com
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GardiniLab@GardiniLab10 May 2019
We're looking for a postdoc interested in using epigenomics to understand enhancer-promoter crosstalk during cell fate committment! DM if interested. Please RT!!!
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MED_COMPLEX@MED_COMPLEX10 May 2019
Architecture of Saccharomyces cerevisiae @SAGA_Complex nature.com/articles/s41421-0…
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SAGA_COMPLEX retweeted
MED_COMPLEX@MED_COMPLEX6 Feb 2019
TFIID-dependent SEC recruitment cell.com/cell-reports/fullte… Come on MED26, you are useless !!!! cell.com/cell-reports/fullte…
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I could not be happier to see our #paperaccepted and #highlighted in @Dev_journal . We only start to understand the functions of the #differentmodules of #SAGAcomplex in #development and our study on Usp22 role has been nothing less than fascinating! dev.biologists.org/content/e…
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SAGA_COMPLEX retweeted
MED_COMPLEX@MED_COMPLEX29 Jan 2019
Transcription Factor-Mediator Interfaces: Multiple and Multi-Valent. dlvr.it/QxksLm
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MED_COMPLEX@MED_COMPLEX24 Jan 2019
Drosophila Mediator subunit Med1 is required for GATA-dependent developmental processes: divergent binding interfaces for conserved coactivator functions. dlvr.it/QxNJqH
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Phase separation Of nucleosome arrays Blocked by acetyl #ChromatinHaiku biorxiv.org/content/early/20…
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Replying to @domhelmlinger @biorxivpreprint @SAGA_Complex
4/4 Last, @SAGA_Complex assembles through an ordered pathway. Spt20 recruits Tra1 first, which then promotes incorporation of the DUB module. Congrats Alberto, @DamToullec, Céline, Gwenda from @CRBM_Montpel !
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Replying to @domhelmlinger @biorxivpreprint @SAGA_Complex
3/4 Second, TTT chaperone binds Tra1 cotranslationally, to promote its folding and maturation *before* its assembly into both SAGA and NuA4. This is therefore a different mechanism from the recently published cotranslational binding of interacting partners.
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Replying to @domhelmlinger @biorxivpreprint @SAGA_Complex
2/4 First, Tra1 requires a dedicated chaperone machinery, called TTT, for assembly into @SAGA_Complex . Tra1 is a pseudokinase of the PIKK family and shares this chaperone with active active PIKKs, incl. mTOR, ATM, and ATR. Likely why Tra1 "stayed" PIKK-like.
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Latest manuscript from our lab is out at @biorxivpreprint ! Very proud of my group for this accomplishment. We discovered several principles that govern assembly of the @SAGA_Complex . Thread 1/4
bioRxiv@biorxivpreprint19 Jan 2019
Chaperone-mediated ordered assembly of the SAGA transcription complex biorxiv.org/cgi/content/shor… #bioRxiv
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Are you interested in #chromatin & #RNA fate?come to wonderful #Valencia and Apply for a 3-yrs #JuandelaCierva postdoctoral contract with the Rodriguez-Navarro Lab @IBV_CSIC @DPE_CSIC @FJIprecarios @piratas_ciencia @generegulation @pelechanolab
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