Associate Professor @ResearchUGent @UGent_HSR @microscopyUGent 'FLIM gypsy' and developer of #FLIM #PLIM bio-/nanosensors for #organoids #3D PI @FlImagin3_DN
Ghent University, Belgium
Joined January 2010
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#NewPaperAlert now we have this study published in @angew_chem Proud to be involved in such an interesting study shedding a new light on design of solvatochromic and hydration-sensing fluorescent dyes! This new generation of #BODIPY dyes offers everything that [1/3]
#Preprint alert! Together with Filatov's group (TUD), we report a new type of O-BODIPY dyes displaying exceptional brightness & fluorescence lifetime range sensitive to... water! chemrxiv.org/doi/full/10.264… works great in #FLIM with cells, spheroids & organoids! @microscopyUGent
Excited to speak about #FLIM & #organoids at the conference on new approaches #NAM for 3D cell culture in Freiburg 16-18 March 2027 organised @DECHEMA A lot of cool keynote speakers already: dechema.de/en/3DCC2027
Looking forward to speak tomorrow at BELSACT Ghent 2026 meeting covering safety, thinking outside the box 3Dcellculture, bioproduction, NAMs, RE-Place and more! My talk will focus on using small intestinal organoids, their live #FLIM for studies of #MNPs @microscopyUGent
Our team will present @FOMconferences 2026! @hang_zhouFLIM and Gabriele representing @microscopyUGent & @FlImagin3_DN will talk about #FLIM #organoids #micronanoplastics #spheroids #dynamicmitochondria & #oxygenation!
#FluorescenceFriday a result of not successful session this morning, second harmonic generation (SHG) image of the collagen I gel, - depending how you look at it - either a dog or some sort of triceratops relative @microscopyUGent #Multiphoton #SHG @FlImagin3_DN
This #FluorescenceFriday I am highlighting our recent article in @JHCnews where we used #FLIM to validate #STED #immunofluorescence & Antibody specificity in localising TIE2 protein in kidney @microscopyUGent : doi.org/10.1369/002215542514…
This #FluorescenceFriday we showcase live 3D #autofluorescence image of iPSC-derived human neural organoids, 21 'days old': multiple #neuralrosette presence &3D structures are evident in cyan (NADH) and pseudocolor #FLIM images. Taken together with @Norevanloon @microscopyUGent
#FluorescenceFriday today we have a snapshot of cell proliferation (S phase labelling by Hoechst, BrdU& #phasorFLIM) imaging in live mouse intestinal organoids. Different colour nuclei indicate different cell cycle stage with #NADH & lumen AF! #workinprogress @hang_zhouFLIM
#FluorescenceFriday an image from our project on #FLIM-based assessment of #nanoplastic pollution with polarity-reverted small #intestinal #organoids. Immunofluorescence of ZO-1 (cyan) together with F-actin (green) and NPs (magenta). With @hang_zhouFLIM @FlImagin3_DN Stay safe!
