Congrats to our grad students who pitched therapeutic targets at this year's @ScrippsGradPrgm Drug Discovery Shark Tank - particularly Shelby @ShelbyFerrier and Morgan @m_m_gee on their winning proposal to target Cholera toxin subunit B, which raised $4 million!

The human mitochondrial ClpXP is uniquely assembled and regulated compared to its bacterial counterparts. Congrats to Kelly Chen on her years of hard work on this challenging complex! biorxiv.org/content/10.1101/…

Proof our lab skills are cutting-edge—both at the microscope and the (pumpkin carving) bench! 🎃🔬 #CryoEM #PumpkinScience @scrippsresearch

Some thoughts on the current state of single particle cryo-EM map/model validation. (TLDR: preferred orientation remains a big problem, ❤️ Q-scores. There's been progress, but there's still more to do. ) authors.elsevier.com/sd/arti…

We always thought nucleotide state strictly defined AAA+ conformation, but Jeff Mindrebo's work on mito LONP1 suggests more is at play - substrate interactions also drive protein translocation! Revisiting & rethinking the hand-over-hand mechanism: tinyurl.com/49y7a6h6

Our first foray into the fascinating field of DNA repair! Congrats to postdoc Chris Zerio @chriszerio, who tested hundreds of oligos to identify one that trapped the helicase of human Polymerase θ in the process of annealing two strands of DNA. tinyurl.com/jaddar9u

Congrats to all the participants of the 2024 @cshlcourses cryoEM course, and special congrats to Team 3: @Nancy_Jaiswal23, @Denjoi1, Edem Gavor, the winners of this year’s competition! We hope you all had a great time and share the knowledge you’ve gained with others!

Kicking off the 2024 Cold Spring Harbor CryoEM course @cshlcourses! It’s going to be a grueling but amazing 2 weeks!

Further evidence of high-resolution capabilities at 100 keV with a side-entry cryo holder using the @GatanMicroscopy Alpine detector. It even performed better than the K3 on a small sample - Great collab with colleagues in Verba lab @UCSF biorxiv.org/cgi/content/shor…

Symmetric transthyretin (55 kDa) has been crystallized hundreds of times - why look at it by cryoEM? Good thing @BenjaminBsnt did! Asymmetry, distinct binding pockets, multiple conformations - explains negative cooperativity & amyloidogenic pathway: tinyurl.com/yf67muvv