@JSamWilson

BBSRC Discovery Fellow | #CryoEM 🔬 | Phages - Antimicrobials - Host Takeover | He/Him

Joined August 2009
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🎉🎉 🥳 We are very excited to finally share with everyone our latest preprint on a fascinating bacteriophage infecting C. difficile, with 12 structures from a 23 MDa phage! and a few surprises along the way. Let us take you on a tour of phiCD508… A thread 🧵 1/9
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🚨 Funded PhD Opportunity - Cryo-EM & Structural Biology (Sheffield, UK) Study toxic bacteriophage proteins targeting drug-resistant Enterococcus. Training in cryo-EM, AlphaFold, protein biochemistry & infection biology. 🔗 Apply by 7 Jan 2026 More info: yorkshirebiosciencedtp.ac.uk
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Contact myself + Prof. Per Bullough for more info
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Jason wilson retweeted
Paul CM Fogg@PCMFogg27 Nov 2024
We have a fascinating preprint just out, revealing the widespread packaging of bacterial genomic DNA into capsids in the human gut:bit.ly/3B2ajza. Major denizens of the #microbiome implicated with broad potential impact on bacterial evolution and horizontal gene transfer
Concept summary of the approach used in the present study. A. Diagram of a caesium chloride gradient tube of VLPs purified from human faecal samples. Two bands of different densities are illustrated, followed by a flow chart of the Oxford Nanopore sequencing process and an illustrative chart showing read length distributions typical of phage, transducing phage particles and gene transfer agents. B. Distributions of read lengths obtained from six faecal VLP fractions.

ALT Concept summary of the approach used in the present study. A. Diagram of a caesium chloride gradient tube of VLPs purified from human faecal samples. Two bands of different densities are illustrated, followed by a flow chart of the Oxford Nanopore sequencing process and an illustrative chart showing read length distributions typical of phage, transducing phage particles and gene transfer agents. B. Distributions of read lengths obtained from six faecal VLP fractions.

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Check out our new preprint (biorxiv.org/content/10.1101/…) describing widespread bacterial DNA packaging by #bacteriophages and gene transfer agents (GTA) in the human gut #microbiome. Many thanks to @PCMFogg, @JSamWilson, Pavol Bardy, and @colinhillucc for collaborating with us.
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🧵one of my favorite tools: Foldseek (1/7) We have traced the evo of species by comparing their proteins. We use models that assign a %similarity between seqs and confidently infer that 2 proteins are homologous if share ≥25% SeqSim. But what happens when it’s less than this?
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Very happy to have played a part in this study, great to see it out in preprint form. biorxiv.org/content/10.1101/…

Large scale capsid-mediated mobilisation of bacterial genomic DNA in the gut microbiome

Here, we present a novel approach to study packaging and mobilisation of bacterial DNA in complex microbial communities, such as the human gut microbiome, by viral or virus-like capsids. Transducing...

biorxiv.org
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"Ever wondered how α-latrotoxin, the black widow spider's 🕷️ neurotoxin, turns into a cation-selective channel? Check out our latest manuscript! rdcu.be/dWez3 @NatureComms #Neuroscience #Toxin #cryoEM @Sfb1348 @uni_muenster
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🚀Announcing my first (co)first-author manuscript 🚀 "Cell attachment and tail contraction of S. aureus phage phi812" 🔗bit.ly/Phi812 Check out the 🧵 to explore the structural details of phage interaction with Gram+ bacteria🤯 #PhageTherapy #Research #PhDlife 1/11
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Excited to get started as a BBSRC Discovery Fellow at @ScienceShef in October. I'll be looking to understand how bacteriophages hijack their hosts and how we can exploit that understanding towards new antimicrobials 🔬🦠
Biotechnology and Biological Sciences Research@BBSRC22 Aug 2024
Meet the 15 new BBSRC Discovery Fellows who are receiving a collective £6m of investment for early-career independent research. The fellowship scheme, now in its 10th year, supports promising researchers who aspire to secure the future of biosciences. orlo.uk/c0ih2
Snippet of a quote featured in the news item. 'Dr Robert Hardwick, Interim Associate Director for People, Talent and Culture at the Biotechnology and Biological Sciences Research Council (BBSRC), said: "We are immensely proud to support these fifteen outstanding researchers through our Fellowship scheme. Their projects not only demonstrate remarkable scientific promise, but also the potential to address pressing global challenges. With BBSRC’s support, they are well-placed to lead innovative and impactful research."'

ALT Snippet of a quote featured in the news item. 'Dr Robert Hardwick, Interim Associate Director for People, Talent and Culture at the Biotechnology and Biological Sciences Research Council (BBSRC), said: "We are immensely proud to support these fifteen outstanding researchers through our Fellowship scheme. Their projects not only demonstrate remarkable scientific promise, but also the potential to address pressing global challenges. With BBSRC’s support, they are well-placed to lead innovative and impactful research."'

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A huge thank you also to everyone who supported me during my application 🙏⭐️
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Jason wilson retweeted
Jess Buddle@BuddleJess15 Aug 2024
✨️ It's been a long time coming ✨️ journals.plos.org/plosbiolog… Really excited to finally be able to share the publication of my first first-author research paper in PLOS Biology today! A Thread 🧵

Identification of pathways to high-level vancomycin resistance in Clostridioides difficile that...

Clostridioides difficile is a human pathogen primarily treated with the antibiotic vancomycin. High-level vancomycin resistance is a serious problem in other pathogens, not yet for C. difficile. This...

journals.plos.org
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Fully funded 4-year PhD position using cutting-edge fluorescence and electron microscopy to study the bacterial cytoskeleton. 🔬🦠🦴 Co-supervised by Martin Goldberg (Durham) and Yulia Yuzenkova (Newcastle). Apply by 22 July! findaphd.com/phds/project/bb…

BBSRC NLD Doctoral Training Partnership: Why do bacteria phosphorylate their cytoskeleton? at...

PhD Project - BBSRC NLD Doctoral Training Partnership: Why do bacteria phosphorylate their cytoskeleton? at Newcastle University, listed on FindAPhD.com

findaphd.com
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In the USA in 1943 they produced a film 'Don't be a Sucker' about fascism. It perfectly explains Nigel Farage, Suella Braverman, Tommy Robinson and the entire Right.
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Jason wilson retweeted
Axel Levy@axlevy016 Jun 2024
We present DRGN-AI for fast, ab initio cryo-EM reconstruction! * learns a neural field from unposed images, * designed for single-shot reconstruction of unfiltered datasets, * finds new states missed by prior approaches! Teamwork led by @ZhongingAlong drgnai.cs.princeton.edu/ 1/
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We present a preprint about a “stargate” mechanism of the capsid opening required for genome delivery in members of the Microviridae family. See below for a short manuscript summary (1/9). biorxiv.org/content/10.1101/…

A stargate mechanism of Microviridae genome delivery unveiled by cryogenic electron tomography

Single-stranded DNA bacteriophages of the Microviridae family are major components of the global virosphere. Microviruses are highly abundant in aquatic ecosystems and are prominent members of the...

biorxiv.org
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Check out our latest work on #CRISPR-associated #transposons (CAST) published in @MolecularCell! 🤩 authors.elsevier.com/c/1jCED… (1/21)
Guillermo Montoya@g1_guillermo3 Jun 2024
We want to thank the generous funding from @ERC_Research and @novonordiskfond and that allowed us to complete this work Have a look at the follow-up twetorial by @proteofrankie explaining the findings #crispr #genomeediting #CryoEM #HorizonEU
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You can't find a better Postdoc opportunity anywhere else! Fantastic mentor and amazing science!
Tim Blower@BlowerLab22 Jan 2024
One week to go until close of applications for a postdoctoral position on phage defence with us here in Durham tinyurl.com/mvwtjvxf
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Magnetic Isolation and Concentration (MagIC)-cryo-EM is a new method to enable direct structural analysis of targets captured on nanomagnetic beads, which requires only 0.0005 mg/mL of targets in 100 µL crude solution to make 10 cryo-EM grids. I will explain the magic behind it!
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👇🏼My Husband Sam is looking for new positions in the UK👇🏼, he is a brilliant scientist and would thrive in any #industry position that needs a skilled and enthusiastic #chemist! Share & DM with information about jobs⚛️Thanks for your support! #JobHunting linkedin.com/posts/samuel-nu…
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Seeking scientist volunteers! Want to practice science communication and help author a ✨comic✨ about your research? I need collaborators for this spring's cohort of SciComm & Comics art students! Please share widely. #STEMeducation #Science #sciart
Flyer advertising a need for scientists volunteers. "Call for scientists! Want to collaborate with an art student on a comic about your research? Learn more and sign up by Feb 1st." Simple cartoon heads are wearing hats that are designed to look like items symbolic of different scientific disciplines: an aromatic ring, a crystal, an ammonite, and a paramecium. Link: bit.ly/sciencecomicscollab

ALT Flyer advertising a need for scientists volunteers. "Call for scientists! Want to collaborate with an art student on a comic about your research? Learn more and sign up by Feb 1st." Simple cartoon heads are wearing hats that are designed to look like items symbolic of different scientific disciplines: an aromatic ring, a crystal, an ammonite, and a paramecium. Link: bit.ly/sciencecomicscollab

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