our 5th publication of the year is now out, and it comes with our shortest title yet: ONE microscopy. nature.com/articles/s41596-0… ONE pushes conventional fluorescence microscopy to the molecular scale. It allows us to directly visualize the shapes of individual proteins, resolve conformational changes of around a nanometer, and achieve nanometer to subnanometer imaging precision, while extending these capabilities from purified molecules to complex cellular and tissue samples. This Nature Protocols paper provides the complete workflow for doing this, from sample preparation and expansion to imaging and fluctuation analysis. And now there is ONEpy, our new software package that brings the analysis pipeline together and makes it substantially easier to use. Faster to set up, easier to use, and gives us a much better base for what we want to add next. and this is not all from the team this year. We currently have 3 more papers in revision, and we are preparing a 4th manuscript to submit by the end of the year. we are only getting started, and I could not ask for a more ambitious, creative and relentless team to build this with. what started with ONE as something that seemed almost impossible is now becoming a field: optical structural biology.