@MicroscopyMemes

Memes about #microscopy + image processing/analysis (especially in the context of core facilities and labs)

Joined December 2021
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60Media
Why do my cells look blurry?
Meme: Guy pouring oversized oil container onto salad Text on guy: Person on the scope before you text on salad: 20x objective

ALT Meme: Guy pouring oversized oil container onto salad Text on guy: Person on the scope before you text on salad: 20x objective

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Testing oil objectives with spring-loaded nose cones
cinesthetic.
@TheCinesthetic24 Jan 2024
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New programmers adding comments
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Bring a bucket and a mop for this #NanoPyx
Ricardo Henriques@HenriquesLab15 Aug 2023
🚨🔬👩‍💻🤖Thrilled to share our newest preprint introducing #NanoPyx! - a new bioimage analysis framework capable of 10x speedup through our custom #LiquidEngine #MachineLearning! The brainchild of @Bruno_MSaraiva @inesmcunha @Antonio_DBrito et al (1/9) biorxiv.org/content/10.1101/…
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me (lactose intolerant): Do you have almond milk? Technician making blocking buffer: what
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Biosafety officers looking at this thread
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Most people don't pay attention to acquisition order on #confocal #microscopes. Frequently, nuclear stains like DAPI are the first channel. Instead, let the labelling inform you. For example: Channel 1: Desmoglein 1 Channel 2: Plakophilin 2 Channel 33342: Hoechst 33342
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When your large microscopy data finally uploaded to the data repository and you click on a different section before saving
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Thanks Pfizer
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When I'm checking alignment of the STED scope and Jerry asks what I'm imaging
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When someone says "statistics" in response to "What are you looking to get out of these images?"
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Surely this'll convince sCMOS manufacturers to do something.
Meme: Sad Wolverine left out of party Top: two people kissing with text "(s)CMOS correction algorithms" and "most (s)CMOS cameras) Bottom: a more zoomed out shot with a frowning Wolverine with text: "cameras with manufacturer dark current correction that can't be turned off"

ALT Meme: Sad Wolverine left out of party Top: two people kissing with text "(s)CMOS correction algorithms" and "most (s)CMOS cameras) Bottom: a more zoomed out shot with a frowning Wolverine with text: "cameras with manufacturer dark current correction that can't be turned off"

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What do you do after justifying aims? #grantwriting
Screenshot of Microsoft Word document with title "Grant Proposal" and repeated text "AIMS" that has been justified

ALT Screenshot of Microsoft Word document with title "Grant Proposal" and repeated text "AIMS" that has been justified

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When you interrupt someone mouth pipetting
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Me to my cells as I optimize live-cell imaging conditions
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When deciding which objective to purchase, save time by buying one which has already been scratched.
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How #STED microscopy works
Will Smith hitting Chris Rock Chris Rock: Excited fluorophores Will Smith: Depletion laser

ALT Will Smith hitting Chris Rock Chris Rock: Excited fluorophores Will Smith: Depletion laser

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Actual footage of the fast moving diffraction-limited object that I want to FRAP.
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Every paper on modifying fluorophores be like
Fluorophores: Meme: "We were bad, but now we're good"

ALT Fluorophores: Meme: "We were bad, but now we're good"

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First day working in an advanced light microscopy core facility
Me:*lowers laser power to prevent photobleaching* User trying to FRAP: Unsettled Tom meme

ALT Me:*lowers laser power to prevent photobleaching* User trying to FRAP: Unsettled Tom meme

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When you use a macro to apply scalebars
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