Pascal Vos (@pascal_d_vos) profile picture

Pascal Vos

@pascal_d_vos

Research Fellow | Laboratory of Synthetic Biology and Drug Discovery | The Kids Institute

 Perth, Western Australia
Joined November 2016
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Photo by Pascal Vos (@pascal_d_vos)Photo by Pascal Vos (@pascal_d_vos)
Are you tired of slow gene editing and scattershot targeting? Well no more, here we introduce HyperDriveCas9. An engineered Cas9 variant that is both hyperactive and hyper accurate. doi.org/10.1016/j.crmeth.202…
Image from Pascal Vos (@pascal_d_vos)
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Better CRISPR base editors! More efficient nicking of the unedited DNA strand improves the ability to edit hard to reach bases. @ACSSynBio pubs.acs.org/doi/10.1021/acs…
Image from Oliver Rackham (@TheRackhamLab)
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High-fidelity Cas9 variants that prevent off-target cleavage typically reduce on-target activity. Adding TurboCas9 mutations to these enzymes can improve activity while maintaining off-target fidelity. @CellRepMethods cell.com/cell-reports-method…
Image from Oliver Rackham (@TheRackhamLab)
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Mutational rescue of the activity of high-fidelity Cas9 enzymes dlvr.it/T5NxQL
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“I am a part of all that I have met” – Ulysses; Alfred Tennyson CRISPR screening, volumetric EM and multi-omics reveal the highly connected nature of organelles. Defects in peroxisomes, Golgi and the ER converge on mitochondria via the lipidome. nature.com/articles/s41556-0…
Image from Oliver Rackham (@TheRackhamLab)
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Why are there so many tRNA genes in mammalian genomes? Knockout of the seven-membered phenylalanine tRNA gene family in mice using CRISPR reveals that multiple genes are required for both development and balanced translation. rdcu.be/dadEz #RNA #translation
Image from Oliver Rackham (@TheRackhamLab)
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Honoured to present my paper on improving CRISPR-Cas9 at the last @SbaPerth event of the year!!
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Trick or Treat? @TheRackhamLab explore Frankenstein Cas9 variants and the latest developments in engineering Cas9 for improved gene editing in a new #BiochemSocTrans mini review doi.org/10.1042/BST20220873
Schematic depiction and crystal structure of Cas9

ALT Schematic depiction and crystal structure of Cas9

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Frankenstein Cas9! Here we review the latest developments in engineering Cas9 for improved gene editing portlandpress.com/biochemsoc… #CRISPR #SynBio
Image from Oliver Rackham (@TheRackhamLab)
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Ready to shred DNA with a hyperactive Cas9? We used artificial genetic circuits in yeast to test computationally designed Cas9 variants and found TurboCas9 that can introduce large and diverse indels in mammalian cells. Free to read @NatureComms: rdcu.be/cOFkX #CRISPR
Image from Oliver Rackham (@TheRackhamLab)
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My first research paper from my PhD is officially out now! Cas9 is now officially gone into hyperdrive with our newly designed TurboCas9. @TheRackhamLab @FilipovskaLab nature.com/articles/s41467-0… #Cas9 #Synbio #
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Organization and expression of the mammalian mitochondrial genome go.nature.com/3MqLEV0 #Review by @TheRackhamLab & @FilipovskaLab
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What happens when the master regulators of RNA regulate each other? Check out “Modulation of miRNA function by natural and synthetic RNA‑binding proteins in cancer”. link.springer.com/article/10… rdcu.be/bFGup #miRNA #SynBio #RNA #cancer
Image from Oliver Rackham (@TheRackhamLab)
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What happens when the master regulators of RNA regulate each other? Check out “Modulation of miRNA function by natural and synthetic RNA‑binding proteins in cancer”. link.springer.com/article/10…… rdcu.be/bFGup #miRNA #SynBio #RNA #cancer
Image from Pascal Vos (@pascal_d_vos)
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