Genome editing, synthetic biology and mobile genetic elements.
Western Univ Biochemistry
Joined June 2016
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Happy to be part of this incredibly comprehensive study on reconstructing ancestral homing endonucleases. Congrats to all authors. academic.oup.com/nar/article…
Paper alert! 📢 PHYCUT: multiplexed Cas9 editing for microalgae. pubs.acs.org/doi/10.1021/acs… Amazing work from #EmilyStuckless with assist from #Gloor_lab. Multiplexed knockouts of fucosyltransferase genes for humanization of N-linked glycosylation pathway.
🔊Paper alert! Incredible work from #EmilyStuckless and fellow Edgell lab members on PHYCUT multiplex Cas9 editing system for Phaeodactylum tricornutum. biorxiv.org/content/10.1101/…
PHYCUT used for glycoengineering by knocking out fucosyltransferases as first step in humanizing N-linked glycosylation pathway!
Paper update! Now online pubs.acs.org/doi/10.1021/acs…
at ACS Synthetic Biology @ACSPublications
New paper 📢. What happens when @gary_wfoo genetically entangles a thermally-regulated meganuclease and colE9 immunity protein? Ultra-stable biocontainment in the mammalian gut!! biorxiv.org/content/10.1101/…
David Edgell retweeted
New paper 📢. What happens when @gary_wfoo genetically entangles a thermally-regulated meganuclease and colE9 immunity protein? Ultra-stable biocontainment in the mammalian gut!! biorxiv.org/content/10.1101/…
Paper 🚨📢! Adenine methylation in PAM sequence regulates Staph aureus Cas9 activity in bacteria. biorxiv.org/content/10.1101/…. Superstar collab between #DaltonHam @nortnlentivirus
crisprHAL ML model found poor activity at PAM sites with GATC sequences....seem familiar? GATC is DNA adenine methyltransferase site in bacteria. Large scale in vivo assays, Nanopore sequencing, and in vitro assays all show that G*ATC methylation impacts SaCas9 activity.
All the details in the paper! Another stellar collaboration with #GloorLab. Supported by @CIHR_IRSC @westernuBiochem @SchulichMedDent
New paper 📢. What happens when @gary_wfoo genetically entangles a thermally-regulated meganuclease and colE9 immunity protein? Ultra-stable biocontainment in the mammalian gut!! biorxiv.org/content/10.1101/…
Amazing work by @gary_wfoo with big assist from @deheinrichs. Supported by @CIHR_IRSC @westernuBiochem @SchulichMedDent
Incredibly lucky to have played a small role in this amazing paper. nature.com/articles/s41586-0…. Congrats to all involved!! @SchildPoulter @SchulichMedDent @WesternU #Zhalaboratory @Columbia
Congrats to trainees @THamilton26 @brjoris @nortnlentivirus and collaborators @BogumilKaras @gbgloor @RodrigueLab for their paper being selected as one of the Top 25 ACS papers for World Micrbiome Day!
pubs.acs.org/page/vi/world-m…
David Edgell retweeted
📣 @CanJMicrobio is accepting papers for a collection showcasing Canadian research in #Synthetic_Biology.
🧬More info: ow.ly/wYGx50RKYtK
@CSM_SCM @DavidKwanPhD @BogumilKaras @SynBioCanada @megatev
✨New extended due date! 📅 November 15, 2024
ALT Call for Papers - Canadian Journal of Microbiology
📢New gene editing technology biorxiv.org/content/10.1101/…. This is result of amazing collaboration with @SpecificBio and supported by @NSERC_CRSNG @MitacsCanada @LumiraVentures @adMare_Bio @westernuBiochem @SchulichMedDent 🧵👇
4/n Guess what? Size does matter - everything fits within a single AAV vector (TevSaCas9 + repair guide). NO need for co-delivery of exogenous repair factors, accessory repair proteins/templates, or small/molecules/proteins to modify repair pathways.