Please RT! We are very proud to announce LSFM 2025!  The light-sheet conference/workshop will be held this year June 1st-7th, 2025, at the Mount Desert Island Biological Laboratory (MDIBL) @MDIBL in Bar Harbor, Maine. Application portal open now! Application deadline (rolling) May 12th, 2025. Limited financial assistance is available. mdibl.org/course/lsfm-2025/ This is the only comprehensive conference/workshop of this length in the US. This year, the conference will be 1.5 days and the workshop will be 4 days (more time on the systems!). Workshop space is limited. First come! Confirmed Speakers; Reto Fiolka @RetoPaul - UTSW - Keynote Steve Ross @NikonInst -Nikon Instruments Simon Watkins @simoncwatkins1 -University of Pittsburgh Claudette St. Croix @ClaudetteCroix -University of Pittsburgh Kate McDole - MRC Laboratory of Molecular Biology Holly Gibbs @holly_c_gibbs -Texas A&M Anna-Karin Gustavsson @gustavssonlab -Rice University Stephanie Kramer - Case Western Reserve University Ruixuan Gao @Ruixuan3 -University of Illinois, Chicago Bingying Chen @bingy_C -UTSW Fabian Voight @voigtvision -Harvard University Stephanie Rudolph @StephRudolphLab - Albert Einstein College of Medicine Melissa Cooper @MelCooperPhD -NYU Langone Health Doug Shepard @DougPShepherd -Arizona State Univesity Doug Richardson @Harvard_CBI -Harvard University Emmanuel Reynaud @ReynaudEmmanuel -University College Dublin Kara Cerveny @kara_cerveny -Reed College Carsten Wolff @carsten_wolff -MBL With more to come! Confirmed vendors:  12 systems! 3i ASI Leica Bruker MBF Zeiss Miltenyi LifeCanvas LIT Hamamatsu Abhishek Kumar (co-organizer) - MBL Kate McDole (co-organizer) - MRC Laboratory of Molecular Biology Gary Laevsky (co-organizer) - Princeton University