Postdoc studying planarian regeneration @ the Stowers Institute in Kansas City. My friends call me Biff.
Kansas City, Missouri
Joined July 2021
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Frederick Mann, PhD retweeted
Our new NAFA protocol robustly preserves delicate wounded tissues while facilitating probe/antibody penetration into internal tissues
The fixation protocol is compatible for WISH on regenerating teleost fins, highlighting its versatility
@ScienceStowers @BMCBiology
bmcbiol.biomedcentral.com/ar…
ALT FISH of zpuf-6 (yellow) and DNA staining with DAPI (white). Maximum intensity projection of confocal images, scale bars: 100 μm
ALT ISH in killifish tail fin yields better signal to noise ratio when fixed with PFA + formic acid. A Morphology of the tail fin in the African killifish N. furzeri. Amputation site is indicated by red dashed line. B Chromogenic in situ hybridization of the injury-responsive gene fstl1. 1 dpa and 3 dpa tail fin tissues fixed with 4% PFA and probed for fstl1.C ISH for fstl1 in tail fins fixed with 4% PFA + formic acid. Brightfield images were taken with a stereomicroscope. Scale bars are 500 μm
Frederick Mann, PhD retweeted
The @MBLScience Embryology Course is underway! 🔬🌊 Institute researchers from the @Planarial Lab, @Vidyanandsasi and @DungVuu are teaching assistants at #Embryo24. There is no better place to spend the summer than our satellite lab in #WoodsHole. #marinebiology
#science
Frederick Mann, PhD retweeted
We just posted a new version of our planarian spatial transcriptomics story! Lots of new analysis & RNAi functional testing. Check it out!
@ScienceStowers @fg_mann @DungVuu @S_Nowotarski @Planaria1 biorxiv.org/content/10.1101/…
Frederick Mann, PhD retweeted
Many many congratulations Dr @VirajDoddihal.. your beginners luck for that use of sword epic cOool 😍🤩 happy happy times Dr. Enjoys enjoys the glory of all ur hard work 🍾🥂😃🤩
Frederick Mann, PhD retweeted
Excited to announce the planarian transgenic workshop at Stowers, Nov. 7-10, organized by @Planaria1 and us, fully funded by NSF EDGE. Come to transfect worms and build your own luminescence microscope to monitor gene expression. Register at stowers.service-now.com/visi…. Please RT.
Frederick Mann, PhD retweeted
How does a small fragment of planarian regenerate an appropriately proportioned complete animal?
As part of my PhD project I attempted to address this question with @Planaria1 @fg_mann , Eric Ross, & Sean McKinney @ScienceStowers @HHMINEWS
Preprint: tinyurl.com/2hvb9j4z
Frederick Mann, PhD retweeted
What a year it has been @ScienceStowers !! This year as a post baccalaureate research scholar @GSSIMR has been nothing but amazing to me. I’ve learned SO much and I can only thank the @Planaria1 lab, @fg_mann for being the greatest mentor I could ever ask for.. 1/2
Frederick Mann, PhD retweeted
Today we celebrate Enya @DewarsEnya who a year ago joined our institute @ScienceStowers & our lab as a postbaccalaureate
Enya will pursue her PhD @DukeU this fall
While we will miss her, we wish her a world of new knowledge & discovery will always come to greet her
Go science!
Frederick Mann, PhD retweeted
Dr. Biff Mann (@fg_mann) from @Planaria1 lab will discuss a genome-wide Smed RNAi library, and Pallob Barai from @BethMDuncan lab will speak about an RNase H-based ribodepletion strategy for transcriptomic profiling.
We look forward to seeing you there!
2/2
Check out our new preprints: we did Spatial Transcriptomics on regenerating planarians!
Stem cells partner with matrix remodeling cells during regeneration biorxiv.org/content/10.1101/…
@BlairBenhamPyle and I co-led this work with a lot of help from Carolyn Brewster, @DewarsEnya, @S_Nowotarski, Carlos Guerrero-Hernandez, @cindy_maddera, Shiyuan Chen, Jason Morrison, @BrianSlaughter4, Anoja Perera, and @planaria1.
Two more important contributions to recognize for our Slide-seq preprint: the expertise of Seth Malloy @burntmyfingys11 and Kate Hall, who were essential parts of getting this project done! We are very lucky to have been able to collaborate with them @ScienceStowers .
Frederick Mann, PhD retweeted
All of us will be presenting this work at this Friday’s #FlatwormFriday! So tune in!
Relatedly, our Slide-seq work was only possible because the incredible Carolyn Brewster created Syrah, a computational pipeline augmentation for Slide-seqV2. @BlairBenhamPyle, @planaria1, and I have been so lucky to be a part of this.
biorxiv.org/content/10.1101/…
Frederick Mann, PhD retweeted
It was a great experience writing a chapter on diverse types of #regeneration with @Planaria1 @ScienceStowers . Can’t wait to get my hands on this book to read all the other exciting chapters.Thanks for the opportunity @CSHLPress @Ken_Poss_Lab @FoxLabDuke. Loved the cover page!
Hot of the @CSHLPress ! New #regeneration book! Thanks @Ken_Poss_Lab for the opportunity to co-edit. cshlpress.com/default.tpl?ac…
Frederick Mann, PhD retweeted
Congratulations to @planaria1 for his election to #AAASFellow by @AAAS. Fellows are selected for their achievements & extraordinary efforts on behalf of the advancement of science.
Read more about the awardees here: aaas.org/news/aaas-honors-ou…
Frederick Mann, PhD retweeted
Sonpho et al. provide an experimental approach for identifying both known and novel ECM components involved in regeneration — @Planaria1 @ScienceStowers @MahidolU #ECM #TeamMassSpec
mcponline.org/article/S1535-…
Have a look at our new paper, which reports unexpected roles for Hox genes in adult planarians! This beautiful surprising project was led by @cparnoldphd and we are all so happy to share it.
From the laboratory of @Planaria1 @ScienceStowers, our paper identifying unsuspected roles for Hox genes in adult tissues is now out in @NatureComms. Here is a summary thread of the story. @HHMINEWS @NIGMS. #hoxgenes #invertebrates
1/16
nature.com/articles/s41467-0…
Frederick Mann, PhD retweeted
From the laboratory of @Planaria1 @ScienceStowers, our paper identifying unsuspected roles for Hox genes in adult tissues is now out in @NatureComms. Here is a summary thread of the story. @HHMINEWS @NIGMS. #hoxgenes #invertebrates
1/16
nature.com/articles/s41467-0…
Frederick Mann, PhD retweeted
Sharing the latest preprint from @Planaria1 lab describing a protocol for WISH and immunostaining while preserving delicate tissues. Carlos is the superstar behind this protocol and was such a fun collaboration with @fg_mann
@ScienceStowers @GSSIMR
tinyurl.com/7xwssp3n