We create unique tools based on #nanobodies to advance #science & #research 🦋https://xitter.cf/t.co/lHqWbs3zQ9
Göttingen, Germany
Joined December 2016
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Spontaneous blinking. OneStep IF. Precise #SMLM. 🙌
Our Smart Secondaries® JF635b ✨ combine a self-blinking dye with small secondary #nanobodies 🦙 for reduced linkage error, no clustering & fast OneStep IF.
For #dSTORM & #MINFLUX 🔬
Learn more 👉 bit.ly/4ffIkdB
✨🔬Want to see our JF635b-conjugated #nanobodies in action?
📄 Check out the recent NanoLetters paper by Samrat Basak @samub28, @RomanTsukanov, @FelipeOpaz_o et al., doi.org/10.1021/acs.nanolett…
Greetings from #Horizons2026 in our hometown Göttingen! 🦙🔬Our team is here showcasing our #nanobody -based technologies and services. Come by our booth to explore how #NanoTag might support your research!
#SingleDomainAntibody #FluoTag
#SmartSecondaries #ALFAsystem
🔬🫧🧠 NEW: Targeting luminal #Syt1 with flexible readouts powered by the #ALFAsystem! Meet our new sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843) – combine it with different #NbALFA-based reagents for fluorescence imaging or EM.
🔗 Explore more: nano-tag.com/product/sdab-an…
ALT Inside synaptic vesicle lumen sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843, NanoTag Biotechnologies) binds to the luminal domain of Syt1. The ALFA-tag is detected by NbALFA coupled to application-specific labels, e.g. fluorophores or nanogold particles.
🔬📄 You can precomplex the sdAb anti-Syt1 Luminal Domain ALFA-tag fusion with fluorescent NbALFA (FluoTag®-X2 anti-ALFA) or with gold-conjugated NbALFA. The latter approach was demonstrated in a recent paper by @euforna et al. link.springer.com/article/10…
🔬Exciting to see our sdAbs in new microscopy approaches! In recent Nature Biotechnology @YongdengZhang & colleagues introduce me4Pi-SMLM, achieving near-isotropic 3D localization precision of 2–3 nm. Happy to see our anti-ALFA, anti-GFP & anti-RFP sdAbs used in this work! #SMLM
📸 Fig. 3: Two-colour me4Pi-SMLM imaging.
📄Read the paper here: doi.org/10.1038/s41587-026-0…
🔬🫧💚 #FluorescenceFriday New Product –
sdAb anti-Syt1 Luminal Domain pHluorin fusion!
A pH-sensitive reporter combining our sdAb anti-Syt1 Luminal Domain with superecliptic pHluorin for real-time monitoring of #SynapticVesicles exo-endocytic cycling. ♻️
#Neuroscience #Nanobody
🔗 Learn more about the 𝘀𝗱𝗔𝗯 𝗮𝗻𝘁𝗶-𝗦𝘆𝘁𝟭 𝗟𝘂𝗺𝗶𝗻𝗮𝗹 𝗗𝗼𝗺𝗮𝗶𝗻 𝗽𝗛𝗹𝘂𝗼𝗿𝗶𝗻 𝗳𝘂𝘀𝗶𝗼𝗻 reagent and explore the live-cell labeling protocol here: nano-tag.com/product/sdab-an…
📄 Read the comprehensive characterization of NbLumSyt1 (= sdAb anti-Syt1 Luminal Domain) toolset, recently published by Goel, Fornasiero @euforna et al. in the Journal of Nanobiotechnology @SpringerNature:
link.springer.com/article/10…
👋 ¡Hola from Barcelona! 🇪🇸☀️ Great start to #FENS2026! Visit NanoTag at Booth #43 to explore our #nanobody tools for #Immunofluorescence, #OneStepIF, #Multiplexing, #SuperResolution & #Pulldowns.
Our team looks forward to meeting you!🦙🧠✨
#SingleDomainAntibodies #Neuroscience
🇪🇸Heading to #FENS2026 in Barcelona? Visit #NanoTag at Booth #43!
Discover our in-house developed #nanobody reagents for imaging, protein analysis, and #neuroscience – from FluoTags, Smart Secondaries, rHcAbs to the ALFA System and Selector Resins 🦙🧠🔬.
💬 Stop by for a chat!
Heading to #FEBS2026? Stop by Booth #7!
#NanoTag team will be there to discuss how our #nanobody technologies can support your research.
Explore the ALFA system, FluoTags, Smart Secondaries, rHcAbs & Selector Resins 🦙🔬🧪
See you in Maastricht!👋
#Microscopy #ProteinAnalysis
Congratulations @syncellbiolab on this impressive study! It's exciting to see IntAct-U-ExM revealing actin architectures with such remarkable detail. Happy to see our #ALFAsystem enabling robust post-expansion labeling & high-resolution visualization of complex actin networks🦙🔬
Our new paper in @PLOSBiology introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by @anubhav_dhar along with an amazing team! #actin #UExM
journals.plos.org/plosbiolog…
Did you know? #NanoTag provides #CRO services for custom single-domain antibody (#sdAb) discovery & protein production🦙🧬🧑🔬
Meet our CEO at #BIO2026 in San Diego this week at the @german_pavilion or get in touch via our website to discuss your project.
#VHH #ProteinProduction
NanoTag Biotechnologies retweeted
Multiplexing with primary antibodies from the same species has always been a challenge. The Premix&Stain protocol with Smart Secondary Nanobodies addresses this directly. And here is a 5-color example showing what that looks like in practice.
Three targets were labeled using mouse primary antibodies, detected with Smart Secondaries (FluoTag-X2 anti-mouse) plus direct GFP tag labeling for a fourth channel via FluoTag-X2 anti-GFP. Phalloidin STAR BLUE serves as a structural counterstain for actin.
⬜Actin: abberior STAR BLUE Phalloidin
🟥NUP target 1 (GFP-tagged): FluoTag-X2 anti-GFP abberior STAR 550
🟩Golgi: FluoTag-X2 anti-mouse abberior STAR ORANGE
🟦NUP target 2: FluoTag-X2 anti-mouse abberior STAR RED
🟧Mitochondria: FluoTag-X2 anti-mouse abberior STAR deepRED
These images showcase two new additions to the nanobody conjugate portfolio, developed in collaboration with NanoTag Biotechnologies: STAR deepRED and STAR 550 conjugates extend the spectral range of the FluoTag toolbox. Find the full portfolio in the comments.
#SmartSecondaries #PremixAndStain #STARdyes #FluoTag #abberior #NanoTagBiotechnologies #MulticolorImaging
