@WesleyWongLab

We study the physical basis behind how biological systems work at the nanoscale, focusing on the role of force, using single-molecule and nanoscale methods

 Harvard Medical School
wonglab.tch.harvard.edu
Joined October 2021
  • Tweets66
  • Following51
  • Followers367
8Media
Our light guided molecular patterning method for high-throughput single-molecule studies just published today in Small! Programmable, covalent, multiplexed, accessible. doi.org/10.1002/smll.2025110…

Light‐Guided Molecular Patterning for High‐Throughput Single‐Molecule Mechanical Characterization

A light-guided molecular patterning method is presented for high-throughput single-molecule characterization. The approach enables scalable and accessible molecular patterning without specialized...

onlinelibrary.wiley.com
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What better way to start the year then with a deep dive into multiplexed force spectroscopy? Check out our review article that just came out in Annual Reviews of Biophysics! annualreviews.org/content/jo…

Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy

Single-molecule techniques have transformed biological research by enabling direct observation and manipulation of individual molecules. These methods overcome ensemble averaging inherent in bulk...

annualreviews.org
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Our work on characterizing G-quadruplexes at the single-molecule level is now out in Nucleic Acids Research! academic.oup.com/nar/article…

Single-molecule mechanostructural fingerprinting of nucleic acid conformations

Abstract. Understanding the three-dimensional structure and mechanical response of biomolecules is key to uncovering their molecular mechanisms, particular

academic.oup.com
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Just published in ACS Central Science—our latest work using the Centrifuge Force Microscope to quantify immune-cell interactions. The new setup combines high-throughput force spectroscopy with multichannel fluorescence for single-cell studies. pubs.acs.org/doi/10.1021/acs…
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Wesley Wong Lab retweeted
Taekjip Ha@taekjip28 Jun 2025
high-throughput single-molecule platform to study DNA supercoiling effect on protein–DNA interactions academic.oup.com/nar/article…
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New preprint on single-molecule mechanostructural fingerprinting! doi.org/10.1101/2025.05.08.6… We use DNA nanoswitch calipers to characterize the geometry and mechanical stability of telomeric DNA G-quadruplexes. #Biophysics #SingleMolecule #Mechanobiology #DNAnanotech
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Excited to share our new preprint! It's a light-guided approach for precise and programmable molecular patterning, enabling multiplexed single-molecule force spectroscopy—no lithography needed. Read more at @biorxivpreprint: doi.org/10.1101/2025.04.30.6… #Mechanobiology #SingleMolecule
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Check out our latest manuscript on high-throughput force spectroscopy! We've developed a next-generation CFM with multichannel fluorescence capabilities to study the the avidity of immune-cells at the single cell level biorxiv.org/content/10.1101/…
bioRxiv Biophysics@biorxiv_biophys28 Feb 2025
High-Throughput Centrifuge Force Microscopy Reveals Dynamic Immune-Cell Avidity at the Single-Cell Level biorxiv.org/content/10.1101/… #biorxiv_biophys

High-Throughput Centrifuge Force Microscopy Reveals Dynamic Immune-Cell Avidity at the Single-Cell...

Cell-cell binding, mediated by the physical interactions of receptors and their ligands, plays a fundamental role in immune processes such as immune surveillance and T-cell activation. However,...

biorxiv.org
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Wyss Institute@wyssinstitute25 Apr 2024
To celebrate #DNADay, William Shih, @WesleyWongLab, & Peng Yin discuss how they're using DNA, what problems they can solve with DNA nanotechnology, and their progress towards the ambitious founding vision of our Molecular Robotics Initiative. wyss.harvard.edu/news/evolvi…

Evolving how we use DNA through nanotechnological innovations

By Jessica Leff  On April 25, 1953, a group of researchers published papers in Nature detailing the molecular structure of DNA, the building block of the genetic code of all organisms, for the first...

wyss.harvard.edu
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Check out our latest work on measuring cell-adhesion with the CFM on Sunday (Exhibit Hall AB at 1:45, 801-Pos/B567), and on structural single-molecule proteomics with nanoswitch calipers on Monday (204AB at 12:35, 958-FlashTalk; Exhibit Hall AB at 1:45, 1601-Pos/B554)
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Just landed in Philly for @BiophysicalSoc Looking forward to Single-molecule and nanoscale subgroups tomorrow! #bps2024.
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Beautiful view! Having a wonderful time at #SMBLesHouches. Thank you @RuedaLab and @NynkeDekkerLab for organizing this amazing conference.
Kasia Tych@BiophysK29 Jan 2024
What a view to wake up to! Looking forward to meeting everyone and hearing about all of the exciting single-molecule science at the #SMBLesHouches meeting.
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It was such an enjoyable and lively dinner! Thank you @cees_dekker for flying here to speak!
Cees Dekker@cees_dekker12 Jan 2024
One great aspect of science is its very international character where you get to meet colleagues/friends from all over the world - in this picture @WesleyWongLab @JoeLoparo @shihlab @taekjip & Sua Myoung at dinner after my seminar at Harvard.
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Delighted to host @cees_dekker today for the BCMP Seminar! Packed house and lively discussions!
Harvard Med BCMP@HMS_BCMP10 Jan 2024
Please join us in-person for the BCMP seminar on Thursday, January 11 at 12:30pm by Cees Dekker, KNAW Royal Academy Professor, Dept of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology. @tudelft
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Single-molecule 3D reconstruction for structural proteomics--check out our latest work just published in JACS @J_A_C_S! pubs.acs.org/doi/10.1021/jac…
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Going to the @BiophysicalSoc meeting 2024 in @PhiladelphiaGov? Interested in Single Molecule Mechanisms? In Tweezing & Visualizing one molecule at a time? Then you are invited to our subgroup dinner on Sat evening, 2/10/24! Can't wait to see you all there - tickets subsidized!
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Congratulations to @ardemp and the team on this beautiful work! And so proud of my former student Eric!
Scripps Research
@scrippsresearch17 Aug 2023
A new study by @ardemp and colleagues shows how the cellular environment can shape the structure of the mechanosensor #PIEZO1, revealing the basic molecular movements underlying channel activation scripps.edu/news-and-events/… @Nature
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Wesley Wong Lab retweeted
BlacklowLab@BlacklowLab31 Jul 2023
Congrats to Colin with a special thanks to Emily and Khal!
Cell@CellCellPress28 Jul 2023
Now online! Structural basis for membrane-proximal proteolysis of substrates by ADAM10 dlvr.it/SstpJ6

Structural basis for membrane-proximal proteolysis of substrates by ADAM10

Membrane-proximal proteolysis by ADAM10 depends on both relief of autoinhibition and distance measurement by its partner tetraspanin.

cell.com
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Check out the preprint of our newest work on single-molecule proteomics! biorxiv.org/content/10.1101/… Here, we use mechanical force to reconstruct the 3D geometry of a natively folded protein complex at the single-molecule level.

Mapping Single-molecule Protein Complexes in 3D with DNA Nanoswitch Calipers

The ability to accurately map the 3D geometry of single-molecule complexes in trace samples would lead to new insights into molecular mechanics and provide an approach for single-molecule structural...

biorxiv.org
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This extends our previous work on DNA Nanoswitch Calipers from denatured to folded proteins, and incorporates a force-based barcoding system for higher effective resolution. nature.com/articles/s41565-0…
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We are excited about the implications of this work on enabling mechanostructrual proteomics. Wonderful collaboration with the William Shih Lab.
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