@Stjle

Synthetic Biology, Artificial Cells, Amphiphile Systems, Machine Learning, Cell-Free Protein Synthesis: Italy, Denmark, Japan, USA, France, Germany, UK.

 Rome, Lazio
scholar.google.co.uk/citations?user=u6OhA2oAAAAJ&hl=en
Joined April 2010
  • Tweets1.5K
  • Following877
  • Followers553
25Media
Pleased to share my latest review on Cell-Free Protein Synthesis #CFPS and #AI/ML for accelerating drug discovery sciencedirect.com/science/ar… #CellFree #SynBio
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shimizu@musaibouOct 15
On chip 50S assembly pubs.acs.org/jacsat/article/…

Autonomous Biogenesis of Early and Late Assembly Intermediates of a Large Ribosomal Subunit in a...

Abstract. Cell-free autonomous biogenesis of ribosomes is important for the bottom-up assembly of an artificial cell and for engineering of functional ribo

pubs.acs.org
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Photo-Activatable RNA Switches for Optical Control of Cell-Free Protein Synthesis pubs.acs.org/asbcd6/article/…

Photo-Activatable RNA Switches for Optical Control of Cell-Free Protein Synthesis

Abstract. Programmable RNA switches enable target-dependent regulation of cell-free protein synthesis. Here, we report a photo-activatable RNA switch using

pubs.acs.org
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Modular Cell-Free Extract Mixing Enables Soluble, Active Biomanufacturing of Difficult-to-Express and Aggregation-Prone Proteins biorxiv.org/content/10.64898…

Modular Cell-Free Extract Mixing Enables Soluble, Active Biomanufacturing of Difficult-to-Express...

Cell-free protein synthesis (CFPS) is a powerful platform for rapid protein prototyping and biomanufacturing, but expression of complex proteins remains limited by labor-intensive extract preparation...

biorxiv.org
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Gene-driven self-morphing microtubule-based active matte nature.com/articles/s41563-0…

Gene-driven self-morphing microtubule-based active matter

Nature Materials - Cell-free gene expression is embedded directly within a microtubule network to generate a genetically programmed active material with complementary biochemical and physical...

nature.com
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Cells, who needs them? Biochemists turn to proteins in a tube nature.com/articles/d41586-0…

Cells, who needs them? Biochemists turn to proteins in a tube

Nature - Scientists are using cell-free transcription and translation systems to synthesize proteins, design biology-based sensors and develop synthetic cells.

nature.com
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Filippo Caschera retweeted
Riku Nagai@riku_nagai_eduOct 3
Congrats to everyone involved! frontiersin.org/journals/mol…

Frontiers | Full-protein release assay in a reconstituted yeast translation system using GGQ-meth...

Translation termination in eukaryotes requires the concerted action of release factors eRF1 and eRF3, together with accessory factors including eIF5A and Rli...

frontiersin.org
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Filippo Caschera retweeted
Olivier Borkowski@O_BorkowskiOct 2
Mitigating the deleterious impact of saliva on cell-free expression sensors for point-of-use diagnostics doi.org/10.1093/synbio/ysag0…
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A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal inhibitor that binds the peptidyl transferase center nature.com/articles/s42003-0…

A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal inhibitor that...

Communications Biology - A human cell-free translation screen identifies the fungal toxin NT-2 as a ribosomal inhibitor that binds the peptidyl transferase center and is associated with...

nature.com
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Filippo Caschera retweeted
Olivier Borkowski@O_BorkowskiSep 25
Cell-free Engineering of LPST153 Bacteriophage Receptor-Binding Proteins Enables Tunable Host-Range Modulation doi.org/10.1021/acssynbio.6c…
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Cell-free systems enable mechanistic characterization of genetically encoded RNA strand exchange circuits for programmable protein expression biorxiv.org/content/10.1101/…

Cell-free systems enable mechanistic characterization of genetically encoded RNA strand exchange...

Nucleic acid circuits are powerful tools for programming biology, but the design principles for operating these circuits in complex cellular environments remain poorly understood compared to simple...

biorxiv.org
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Cell-free membrane systems reveal electrostatic control of ion transport in TMEM165 and disease-associated CaCA2 variants biorxiv.org/content/10.64898…

Cell-free membrane systems reveal electrostatic control of ion transport in TMEM165 and disease-a...

Ion transport across intracellular membranes is essential for cellular homeostasis, yet organellar transport mechanisms remain difficult to resolve in native cells. Here, we establish a cell-free...

biorxiv.org
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Cell-free systems as complementary Test layers for protein engineering in biofoundry workflows sciencedirect.com/science/ar…
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Filippo Caschera retweeted
Olivier Borkowski@O_BorkowskiSep 15
Engineered Orthogonal Translation Systems from Metagenomic Libraries Expand the Genetic Code doi.org/10.1021/acssynbio.6c…
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visualize.jove.com/42729265-…

A liquid handling platform for standardized quantification of cell-free enzymatic activity encoded...

Rapidly characterize antimicrobial resistance genes (ARGs) with a new semi-automated workflow. Essential for combating AMR and developing new antibiotics.

visualize.jove.com
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A High-Throughput Assay to Identify Specific Nascent Chain Inhibitors biorxiv.org/content/10.64898…

A High-Throughput Assay to Identify Specific Nascent Chain Inhibitors

“Undruggable” proteins without surface-accessible binding sites pose significant challenges to target-based drug discovery. Innovative approaches are needed to tackle these proteins. One promising...

biorxiv.org
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Filippo Caschera retweeted
Olivier Borkowski@O_BorkowskiSep 7
A Two-step Gene Expression Method for In Vitro Evolution of Translational Proteins doi.org/10.1021/acssynbio.6c…
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Filippo Caschera retweeted
Ichihashi Lab Komaba@KomabaLabSep 5
櫻井・庄司・市橋でやった論文が出ました。ドロプレット内反応を2段階で行うことで、翻訳タンパク質の完全in vitro進化ができるようになりました。WTより比活性の高いEF-Gも作れました pubs.acs.org/asbcd6/article/…

A Two-step Gene Expression Method for In Vitro Evolution of Translational Proteins

Abstract. Improving the reconstituted translation system is a key requirement for bottom-up synthetic biology. Here, we developed a two-step in vitro evolu

pubs.acs.org
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Autonomous Homeostatic Synthetic Cells via Self-Gating DNA Nanopores biorxiv.org/content/10.64898…

Autonomous Homeostatic Synthetic Cells via Self-Gating DNA Nanopores

Homeostasis is a fundamental hallmark of living organisms, arising from the complex interplay between biochemical reactions and regulatory feedback systems. Reconstituting such self-regulating...

biorxiv.org
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Filippo Caschera retweeted
Maerkl Lab@MaerklLabSep 3
Jiami and Laura's paper is out now! In collaboration with the Reddy lab at ETHZ! cell.com/cell-systems/fullte…

High-throughput cell-free profiling of SARS-CoV-2 RBD variants enables rapid and quantitative in...

The team developed a high-throughput platform to rapidly measure how hundreds of viral mutation combinations affect antibody binding, generating 31,740 quantitative affinity data points. The result...

cell.com
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Functional mapping and engineering of the Sec translocon unlocked by a cell-free system | Science Advances science.org/doi/10.1126/scia…

Functional mapping and engineering of the Sec translocon unlocked by a cell-free system

Almost all membrane proteins are inserted or translocated across membranes by the universally conserved Sec translocon. Despite its central role, experimental access to Sec function has remained...

science.org
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