
ALT Representative images of an Oxalis debilis (A) and Chamaecrista fasciculata (B), with a black arrow pointing to the pulvini in each respective image. Images of a leaf in which a single embolism event that propagated through the pulvini is shown in color (C and D) with the corresponding water potential at which the embolism events occurred. All embolism events across the leaf were color-coded by the water potential at which they were observed (E and F). For each species, a color scale (G and H) depicting the water potential with each embolism event that occurred is shown below the corresponding image.

ALT A multipanel figure showing that unlike angiosperm stomata, fern stomata are insensitive to abscisic acid (ABA), and fern guard cell SLOW ANION CHANNEL (SLAC) homologues are not activated by ABA-signalling kinases. (a) Fluorescent images of guard cells of the angiosperm Nicotiana tabacum (tobacco) and fern Polypodium vulgare after current ejection of 1 mM Lucifer Yellow, or 1 mM LY with 1 mM ABA. (b) Brightfield images of the same guard cells up to 20 min after current ejection. (c) Normalised stomatal aperture over 20 mins, showing a decrease in stomatal aperture in response to ABA in N. tabacum but not P. vulgare. (d) Mean whole-oocyte current measurements of SLAC homologues from the angiosperm Arabidopsis thaliana (responsive) and the ferns P. vulgare and Ceratopteris richardii (not responsive), co-expressed with or without ABA-signalling kinases in Xenopus oocytes.


























