@SamBryce_Smith

@ucl PhD Student with Pietro Fratta & Maria Secrier Bioinformatics, RNA-seq, 3'UTRs, all things bio... Biochemistry grad @sheffielduni All views my own

Joined September 2020
  • Tweets102
  • Following503
  • Followers184
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Sam Bryce-Smith retweeted
FrattaLab@FrattaLab4 Oct 2024
Out @ScienceMagazine! New tech to activate ALS gene therapies only in the right cells at the right time - Increased safety to have more options for ALS. We do it by taking advantage of cryptic splicing. science.org/doi/10.1126/scie… @UCLIoN @TheCrick @OscarWilkins16 @MaxZYJChien

Creation of de novo cryptic splicing for ALS and FTD precision medicine

Loss of function of the RNA-binding protein TDP-43 (TDP-LOF) is a hallmark of amyotrophic lateral sclerosis (ALS) and other neurodegenerative disorders. Here we describe TDP-REG, which exploits the...

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Sam Bryce-Smith retweeted
Oscar Wilkins@OscarWilkins163 Oct 2024
Our paper on cryptic splicing-controlled protein expression, which we hope will inspire safer and more adventurous gene therapy approaches for ALS and FTD, has been published in @ScienceMagazine science.org/doi/10.1126/scie…

Creation of de novo cryptic splicing for ALS and FTD precision medicine

Loss of function of the RNA-binding protein TDP-43 (TDP-LOF) is a hallmark of amyotrophic lateral sclerosis (ALS) and other neurodegenerative disorders. Here we describe TDP-REG, which exploits the...

science.org
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Muscles 💪 and AAV 🦠 - what more could you ask for? Our preprint highlighting strategies for effective transduction of skeletal muscle with AAV with @wenanlan @J_N_Slayer @FrattaLab : biorxiv.org/content/10.1101/… @UCLIoN @UCLBrainScience @UCLDivofSurgery @TheCrick @UKDRI

Refining gene delivery to skeletal muscle with a dual-strategy approach of muscle-tropic AAV...

Viral vector technologies based on adeno-associated virus (AAV) have demonstrated promising ability to deliver genetic cargo to a range of organs in vivo, with several novel candidates showing...

biorxiv.org
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Sam Bryce-Smith retweeted
FrattaLab@FrattaLab24 Jun 2024
We find TDP-43 loss to cause presynaptic defects, which are rescued by ASOs correcting a single cryptic exon in UNC13A. Surprising effect of just one cryptic exon and promising strategy for ALS! Matt Keuss @PeteHarley95 in great collaboration with @jbneuro biorxiv.org/content/10.1101/…

Loss of TDP-43 induces synaptic dysfunction that is rescued by UNC13A splice-switching ASOs

TDP-43 loss of function induces multiple splicing changes, including a cryptic exon in the amyotrophic lateral sclerosis and fronto-temporal lobar degeneration risk gene UNC13A , leading to nonsens...

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Sam Bryce-Smith retweeted
Maria Secrier@mariasecrier28 May 2024
Final days to register to our symposium on the Spatial Biology of Cancer. Hear from leading experts in the field, and enjoy an exciting Hackathon! meetings.embo.org/event/24-c… #spatial #biology #EMBO
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Demis -- I think AlphaFold3 is really exciting. As Reviewer #3, I got great results from the server. I tried hard to get @Nature to urge you to release the code but was unsuccessful. I did not get it for re-review so I don't know if you responded. So why no code? @GoogleDeepMind
Demis Hassabis
@demishassabis8 May 2024
Thrilled to announce AlphaFold 3 which can predict the structures and interactions of nearly all of life’s molecules with state-of-the-art accuracy including proteins, DNA and RNA. Biology is a complex dynamical system so modeling interactions is crucial blog.google/technology/ai/go…
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Sam Bryce-Smith retweeted
Giulia Tyzack@giulia_tyzack30 Apr 2024
Still looking for a talented and enthusiastic bioinformatician to work with us on a super exciting project on DNA damage and RNA metabolism in motor neuron disease. @PataniLab All details here: ucl.ac.uk/work-at-ucl/search… closing on 9th may! @UCLIoN @TheCrick

UCL – University College London

UCL is consistently ranked as one of the top ten universities in the world (QS World University Rankings 2010-2022) and is No.2 in the UK for research power (Research Excellence Framework 2021).

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Sam Bryce-Smith retweeted
Simon Duerr@simonduerr18 Apr 2024
In order to further the development of an open database of scientific illustrations for the life sciences we have now set up a non-profit under the umbrella of @OC_Europe 1/ opencollective.com/bioicons
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Sam Bryce-Smith retweeted
Jack Humphrey@JackHumphrey_4 Apr 2024
TDP43 proteinopathy exhibits disease, tissue, and context-specific cryptic splicing signatures biorxiv.org/content/10.1101/… Really impressive work from @genentech

TDP43 proteinopathy exhibits disease, tissue, and context-specific cryptic splicing signatures

Mislocalization of the nuclear protein TAR DNA-binding protein 43 (TDP43) is a hallmark of ALS and FTD which leads to de-repression and inclusion of cryptic exons. These cryptic exons represent...

biorxiv.org
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📢Calling all UCL cancer researchers! Don't miss Dr Maria Secrier, Associate Professor in Computational Cancer Biology @UCL give the next Computational Cancer Collaboratorium seminar. 🗓️Tuesday 26 March 2024 ⏰11:30am -1:15pm 🗺️Cruciform Building, LT2 eventbrite.co.uk/e/ucl-compu…

UCL Computational Cancer Collaboratorium Seminar Series

On-going seminar series

eventbrite.co.uk
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Sam Bryce-Smith retweeted
FrattaLab@FrattaLab13 Mar 2024
New postdoc opportunity to work on splicing and ALS therapeutics @TheCrick and @UCLIoN. Basic molecular work with true translational potential. 4 days still to apply. jobs.ac.uk/job/DGJ551/resear…
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Sam Bryce-Smith retweeted
Oscar Wilkins@OscarWilkins168 Mar 2024
We're looking for someone with a creative mindset and excellent modern molecular biology skills (DNA manipulation, Illumina, Nanopore etc) to join our team at @ucl and @TheCrick in the @FrattaLab developing candidate gene therapies for ALS. Apply here: jobs.ac.uk/job/DGJ551/resear…
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Many fully-funded PhDs available with me & @AhmadAlKhleifat @AmmarAlChalabi @sj_marzi (deadline:10th March):surl.li/reuti If you want to analyse multi-omics using ML & bioinformatics to study MND please check out project 21,26,57,58,62,65,74 @KCLDriveHealth @kingsmnd
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Sam Bryce-Smith retweeted
Rahul Satija@satijalab29 Jan 2024
Want to hear about the latest updates/new techniques in single cell and spatial genomics? Check out my lab's eighth annual Single Cell Genomics Day on March 29. Keynotes from @IdoAmitLab @JD_Buenrostro and Xiaowei Zhuang. Please RT/spread the word! satijalab.org/scgd
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Sam Bryce-Smith retweeted
FrattaLab@FrattaLab24 Jan 2024
TDP-43 loss causes mis-splicing in ALS/FTD. We now show it also induces widespread cryptic 3'UTRs. This increases RNA stability, translation and function of ELK1 and other TFs. Also provides novel targets for biomarker and therapeutics development. biorxiv.org/content/10.1101/…

TDP-43 loss induces extensive cryptic polyadenylation in ALS/FTD

Nuclear depletion and cytoplasmic aggregation of the RNA-binding protein TDP-43 is the hallmark of ALS, occurring in over 97% of cases. A key consequence of TDP-43 nuclear loss is the de-repression...

biorxiv.org
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Sam Bryce-Smith retweeted
FrattaLab@FrattaLab24 Jan 2024
This work was led by @SamBryce_Smith and a joint effort with @mariasecrier and many others. Parallel efforts from the Gitler and La Spada labs also identify the link between TDP-43 and 3'UTR changes and out now: biorxiv.org/content/10.1101/… biorxiv.org/content/10.1101/…

TDP-43 nuclear loss in FTD/ALS causes widespread alternative polyadenylation changes

In frontotemporal dementia and amyotrophic lateral sclerosis, the RNA-binding protein TDP-43 is depleted from the nucleus. TDP-43 loss leads to cryptic exon inclusion but a role in other RNA proces...

biorxiv.org
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Sam Bryce-Smith retweeted
Ian Sudbery@IanSudbery23 Nov 2023
Come work with us! I have a PhD position available to help understand which mutations in the non-coding parts of coding transcripts have functional consequences in Stem Cells. #phdjobs #Bioinformatics #genomics #miRNA findaphd.com/phds/project/wh…
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Sam Bryce-Smith retweeted
Oscar Wilkins@OscarWilkins1616 Nov 2023
New BioRxiv paper on using cryptic splicing to unlock precision gene therapy activation for ALS, FTD & other TDP-opathies! Key contributions @MaxZYJChien Josette Wlaschin +many others from @ClaireLePichon @ule_lab @FrattaLab labs and more. A thread 🧵 1/13 biorxiv.org/content/10.1101/…

Creation of de novo cryptic splicing for ALS/FTD precision medicine

A system enabling the expression of therapeutic proteins specifically in diseased cells would be transformative, providing greatly increased safety and the possibility of pre-emptive treatment. Here...

biorxiv.org
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Sam Bryce-Smith retweeted
FrattaLab@FrattaLab16 Nov 2023
Want to deliver gene therapies broadly, but activate them only in diseased neurons? TDP-REG "hacks" TDP cryptic mis-splicing to drive expression only WHEN and WHERE needed! Less toxicity, wider therapeutic window, new options for sporadic ALS therapies. biorxiv.org/content/10.1101/…

Creation of de novo cryptic splicing for ALS/FTD precision medicine

A system enabling the expression of therapeutic proteins specifically in diseased cells would be transformative, providing greatly increased safety and the possibility of pre-emptive treatment. Here...

biorxiv.org
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To the axon, RNA regulation, neurotrophin, and cytoskeleton nerds and nerdettes out there: Ta-dah!! :) (Summary thread coming soon). BDNF controls phosphorylation and transcriptional networks governing cytoskeleton organization and axonal regeneration biorxiv.org/content/10.1101/…

BDNF controls phosphorylation and transcriptional networks governing cytoskeleton organization and...

Axonal degeneration underlies neuromuscular disorders and neuropathies. Dysregulation of neurotrophic factors, such as brain-derived neurotrophic factor (BDNF), in the peripheral nervous system has...

biorxiv.org
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