@RapoportLab

@HarvardCellBio @HHMINEWS lab at @harvardmed. We study protein translocation across various cellular membranes and enjoy red sweaters.

 Laboratory of Human Reproduction and Rep…
rapoport.hms.harvard.edu/
Joined July 2018
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Read our latest #research "The deubiquitinating enzyme Otu1 releases substrates from the conserved initiation complex of the Cdc48/p97 ATPase for proteasomal degradation" published in Sci Rep (2026). https://doi. org/10.1038/s41598-026-42811-6
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Read our latest #research "A heterotrimeric protein complex assembles the metazoan V-ATPase upon dissipation of proton gradients", published with @SpringerNature in @NatureSMB nature.com/articles/s41594-0…
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Read our latest #research "Import mechanism of peroxisomal proteins with an N-terminal signal sequence", published with @SpringerNature in @NatureCellBio rdcu.be/elkQ3
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Excited to share our latest work led by our postdoc Dan Zhao! We uncovered how the bifunctional complex of Mnl1 mannosidase and protein disulfide isomerase (Pdi1) initiates ERAD. A long-sought ERAD reductase is finally discovered! nature.com/articles/s41594-0… Huge congratulations!
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Mystery unveiled! Peroxisomal protein import resembles that of nuclear pore! Congratulations to Yuan and Mike for their joint effort! Thanks to @HHMINEWS for their continuous support on this project! www-science-org.ezp-prod1.hu…
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We are thrilled to share our new work published in Nature today reporting a long-sought peroxisomal retrotranslocation channel formed by a ubiquitin ligase complex. Phenomenal work by our postdocs Peiqiang Feng, Xudong Wu and collaborators. nature.com/articles/s41586-0…
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Our story on substrate translocation mechanism by Cdc48 is online now. Check out this Christmas present here authors.elsevier.com/c/1eIIX…. Big thanks to everyone for their excellent contributions.
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@ZhejianJ @jrengen @GygiLab
Check out our new preprint on the translocation mechanism of the holy-grail Cdc48 ATPase. Congrats to @ZhejianJ and Hao Li, as well as to our wonderful collaborators the Engen lab @jrengen , the Marto lab, and @GygiLab !
bioRxiv@biorxivpreprint
22 Oct 2021
Translocation of polyubiquitinated protein substrates by the hexameric Cdc48 ATPase biorxiv.org/cgi/content/shor… #bioRxiv
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Legobodies are online now, with full sequences available! Have fun!👉pnas.org/content/118/41/e211…
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Rapoport Lab retweeted
Jeeyun Chung@JeeyunC
19 Aug 2021
Excited to share our study on the mechanisms underlying selective autophagy of lipid droplets. We showed that Troyer syndrome protein spartin is a key to this! A wonderful collaboration with @synapsepark and @TalleyJLambert. Amazing support:@DamonRunyon biorxiv.org/content/10.1101/…

The Troyer syndrome protein spartin mediates selective autophagy of lipid droplets

Lipid droplets (LDs) are crucial organelles for energy storage and lipid homeostasis. Autophagy of LDs is an important pathway for their catabolism, but the molecular mechanisms mediating targeting...

biorxiv.org
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Check out our new preprint on Making the nanobody Big again for Cryo-EM, spearheaded by Xudong Wu (will start his own lab at Westlake University soon) in the lab.👉 biorxiv.org/content/10.1101/…

Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies)

We describe a general method that allows structure determination of small proteins by single-particle cryo-electron microscopy (cryo-EM). The method is based on the availability of a target-binding...

biorxiv.org
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Check out our new review on protein translocation through a distorted lipid bilayer. authors.elsevier.com/c/1cVIr…
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Want to know what will happen if a membrane protein is too good at generating curvature? Check our new paper nature.com/articles/s41467-0…
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#CryoEM structure of the post‐translational Sec #translocon with substrate bound and X-ray/HDX of the Sec62 subunit. ➡️ @embojournal paper online now! Congrats to @tw_weng, @jingdongcheng, @BangeBalcony and everyone involved! 🎉👏 embopress.org/doi/abs/10.152…
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Rapoport Lab retweeted
bioRxiv@biorxivpreprint
12 Nov 2020
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide Cleavage biorxiv.org/cgi/content/shor… #bioRxiv
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Rapoport Lab retweeted
Cherry Liu@yulucliu
9 Oct 2020
New work from @Salic_Lab on Hh co-receptors. Wonderful PhD work from Brad. Congratulations!
Developmental Cell@Dev_Cell
9 Oct 2020
New Article online now at Dev Cell: Hedgehog Pathway Activation Requires Coreceptor-Catalyzed, Lipid-Dependent Relay of the Sonic Hedgehog Ligand - zpr.io/HyD8s

Hedgehog Pathway Activation Requires Coreceptor-Catalyzed, Lipid-Dependent Relay of the Sonic...

Wierbowski et al. elucidate the pathway that shuttles the SHH morphogen from producing to responding cells. SCUBE releases lipidated SHH but blocks it from directly signaling through PTCH1. Signaling...

cell.com
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Congrats to our former PhD student, Marco Catipovic (now at @GreenLabJHMI), on his paper in @emboreports: "Protease protection assays show polypeptide movement into the SecY channel by power strokes of the SecA ATPase" #ATPase #translocation embopress.org/doi/abs/10.152…
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New work from our next-door neighbors @Salic_Lab ! Congratulations @KP_BioMed and Brad Wierbowski! cell.com/developmental-cell/…

Distinct Cation Gradients Power Cholesterol Transport at Different Key Points in the Hedgehog...

Petrov, Wierbowski et al. show that eukaryotic RND proteins Dispatched1 and Patched1, key regulators of the Hedgehog developmental signaling pathway, are transporters powered by distinct ion gradie...

cell.com
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Rapoport Lab retweeted
ShorterLab@ShorterLab
30 Jul 2020
The SecA motor generates mechanical force during protein translocation: nature.com/articles/s41467-0…

The SecA motor generates mechanical force during protein translocation

Nature Communications - The ATPase SecA drives Sec-dependent protein translocation across the bacterial plasma membrane. Here, the authors combine kinetic translocation measurements with...

nature.com
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