@PopoMicro

Bacterial transposons; Tn7-like elements; CRISPR; 🇹🇼

Joined June 2018
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In eukaryotes, some transposons target telomeres; in Drosophila, they even replace them. Peters lab and Barabas group show that bacteria with linear genomes also harbor diverse telomeric transposons. doi.org/10.1126/science.adp1… @JoePetersLab @BarabasGroup

Telomeric transposons are pervasive in linear bacterial genomes

Eukaryotes have linear DNA, and their telomeres are hotspots for transposons, which in some cases took over telomere maintenance. We identified several families of independently evolved telomeric...

science.org
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TnsBC telomeric transposons are highly diverse, with distinct gene repertoires. Surprisingly, one subgroup includes the Type I-E Cascade + tnsBC system. The tnsBC was initially thought to replace Cas1-Cas2. Spacer analysis supports their telomeric role, often targeting ttrA.
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With ~1/3 of Streptomyces genomes carrying telomeric transposons, these elements compete for the same niche. Identical transposons in different telomeres, both within and between genomes, are occasionally found to be disrupted by other transposons, indicating fierce competition.
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Eukaryotes have linear DNA and their telomeres are hotspots for transposons. Many bacteria are also known to have linear genomes. We identified several families of independently evolved telomeric transposons. 1/7 biorxiv.org/content/10.1101/…

Telomeric transposons are pervasive in linear bacterial genomes

Eukaryotes have linear DNA and their telomeres are hotspots for transposons, which in some cases took over telomere maintenance. While many bacteria also have linear chromosomes and plasmids, no...

biorxiv.org
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Excited to share our recent findings on Tn7 and its fascinating targeting mechanism, now published in Molecular Cell! 🧬🔬Check it out sciencedirect.com/science/ar…
Alba Guarne@alba_guarne
3 Jun 2024
Happy to share that our last story on Tn7 is finally out. Another example of @shen_yao10 talent and a great collaboration with @JoePetersLab @MikePetassi Assembly of the Tn7 targeting complex by a regulated stepwise process sciencedirect.com/science/ar…
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Disclaimer: Photo was digitally edited to include @PopoMicro and @MachadoChacon who couldn't make the photo shot
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It was a pleasure working with @PopoMicro on this review of CRISPR-Cas associated transposons (CAST). Hopefully it gives a sense for the diversity of Tn7-like transposons and CRISPR systems that independently combined.go.shr.lc/444PwFh
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Hats off to the brilliant minds at Feng lab and Gootenberg lab for their awe-inspiring research achievement! (What a competitive field, people always have manuscripts ready at about the same time) biorxiv.org/content/10.1101/…
Feng Zhang
@zhangf
28 Jun 2023
Since the discovery of CRISPR-Cas systems, people have wondered if a similar type of system exists in eukaryotes. Today, we report in @Nature the characterization of Fanzor, a eukaryotic RNA-guided system that can be reprogrammed for human genome editing. nature.com/articles/s41586-0…

Fanzor is a eukaryotic programmable RNA-guided endonuclease

Nature - Fanzor is shown to be an RNA-guided DNA endonuclease, demonstrating that such endonucleases are found in all domains of life and indicating a potential new tool for genome engineering...

nature.com
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Our small discovery just got published 😇. Relatively few cyanobacterial genomes are home to most known families of CRISPR-Cas transposons (CAST). After a careful search, we found a new capture: type I-D CAST~ academic.oup.com/nar/advance… @JoePetersLab
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We thank Dr. Pietrasiak for providing the strain used in this work from the Terrestrial Cyanobacterial Culture Collection (NuMex TCCC). The frozen cell mass sent by Dr. Pietrasiak was even alive! Tough dessert cyanobacteria! @DrylandAlgae
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Peters lab having fun with snow ~~
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PI is bullied!
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@JoePetersLab
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Replying to @PopoMicro @JoePetersLab @kellogg_liz @Nancy_Craig777 @alba_guarne @PhoebeARice @BarabasGroup @Christinahong9
Look what I found! Compliments of the talented @christinerbeck
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Happy CSHL transposable meeting! #cshlTE22 @shsternberg @JoePetersLab @ShreyaKrishnan3 @MarcinNowotny @kellogg_liz @Nancy_Craig777 @alba_guarne @PhoebeARice @BarabasGroup @Christinahong9 Dyda lab
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CRISPR 2022~ Popo happy~
Sam Sternberg@shsternberg
16 Jun 2022
CRISPR 2022 conference was a blast, with lots of exciting new transposon biology! Fitting, to come just before #TransposonDay2022. We didn’t catch everyone for the pic but some key players ⁦@JoePetersLab⁩ ⁦@kellogg_liz⁩ ⁦@fthoffmann⁩ ⁦@MikePetassi⁩ …
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