computational microscopy and tools for brain imaging. Recently moved to @AllenInstitute from @HHMIJanelia
Seattle, WA
Joined August 2018
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CT-inspired two-photon lets us record glutamate activity from hundreds of synapses at a time, at >1000 Hz. Really happy to share this, and there's more to come!
rdcu.be/bMihg
Kaspar Podgorski retweeted
Meet MagLOV: an engineered protein that responds STRONGLY to magnetic fields.
This is a fluorescence timelapse of MagLOV in E. coli. We're waving a (small) magnet under the plate.
Can you tell where the magnet is?
Want some? It's on Addgene now! addgene.org/219957/
Kaspar Podgorski retweeted
I am thrilled to share my first bio paper ever in @AdamEzraCohen lab with super important contributions from @rhodamine110 lab!
We asked the question: How do neurons compute? We focused on L2/3 neurons.
Come for the science, stay for the videos! (1/17)
xitter.cf/biorxiv_neursci/status…
Voltage dynamics of dendritic integration and back-propagation in vivo biorxiv.org/cgi/content/shor… #biorxiv_neursci
Kaspar Podgorski retweeted
We @AllenInstitute for NeurDyn-Ophys are gearing up to launch a platform for in vivo testing of genetic NM/NT/Voltage indicators. We want your opinion on the most suitable AAV serotype to deliver sensors to be tested. Which serotype do you think would be the best fit?
20%AAV2/1
22%AAV2/5
39%AAV2/9
19%AAV.PHP.eB
183 votes • Final results Kaspar Podgorski retweeted
🎉 Thrilled to share 4 yrs of hard work: iAspSnFR preprint at tinyurl.com/38ve4vh9 🔬 GFP-based aspartate sensor, engineered via deep mutational scanning, tested in HEK293 cells. Highly specific for intracellular aspartate! #iAspSnFR #biosensor #aspartate
Kaspar Podgorski retweeted
iGluSnFR3 is published! Check it out⬇️
nature.com/articles/s41592-0…
We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission.
This is #iGluSnFR3 in action detecting minis w/ TTX.
@PodgorskiLab @HHMIJanelia @AllenInstitute
Our group is looking for a Research Associate interested in helping test new fluorescent indicators in vivo. Fun team. Relocation support to Seattle. Know anyone? alleninstitute.org/careers/#…
Kaspar Podgorski retweeted
Folks, first paper of our lab is out!!
We discovered that a couple of trans-synaptic proteins, LGI1 and ADAM23, are acutely translocated to the synaptic surface during firing!
This controls their abundance at clefts, impacting locally glutamate release.
biorxiv.org/content/10.1101/…
Kaspar Podgorski retweeted
We wrote a blog post in 2018 about the latest glutamate sensor - but times have progressed and so must we. @abhi_aggarwal1 updated our post to feature the new newest sensor: iGluSnF from @PodgorskiLab.
hubs.la/Q017BC5b0
Kaspar Podgorski retweeted
Finding your glute-a-mate has never been easier!
#iGluSnFR3
Check out our preprint on iGluSnFR3: biorxiv.org/content/10.1101/…
Inspired by properties of endogenous glutamate receptors, we made an indicator that’s more sensitive and synapse-specific than previous generations.
Recording by @fltomaska and @m8ta, @HHMIJanelia
Check out our preprint on iGluSnFR3: biorxiv.org/content/10.1101/…
Inspired by properties of endogenous glutamate receptors, we made an indicator that’s more sensitive and synapse-specific than previous generations.
Recording by @fltomaska and @m8ta, @HHMIJanelia
Rui Liu (Kleinfeld lab) imaged the very distinct feedforward and recurrent input patterns that come into Layer 4 of mouse cortex (400 um deep) using adaptive optics 2P imaging, showing the utility of iGluSnFR3 for input-output circuit cracking.
If you're still reading, check out the paper!!
biorxiv.org/content/10.1101/…
Special thanks to @ERSchreiter for taking Abhi and me into his lab and teaching us about protein biosensors!
