@NemonicHub

NExt generation MultiphOton NeuroImaging Consortium. NSF NeuroNex hub

nemonic.ece.ucsb.edu/index.php/Main_Page
Joined August 2016
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It was great fun to work on this preprint with @robmlees @ibianco3 @raacampbell @natorlova Darcy Peterka @labrigger @YatsenkoDimitri Che-Hang Yu & @paqio. Hope this helps many people to characterise their 2p microscopes biorxiv.org/content/10.1101/…

Standardised Measurements for Monitoring and Comparing Multiphoton Microscope Systems

The goal of this protocol is to enable better characterisation of multiphoton microscopy hardware across a large user base. The scope of this protocol is purposefully limited to focus on hardware,...

biorxiv.org
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Microscope standards started in 1858, and they’re still constraining optics. But we can break free. Nature Methods also published this little accompanying backstory / commentary on the Cousa: free readcube link rdcu.be/duf7D and regular link nature.com/articles/s41592-0….
Spencer LaVere Smith@Labrigger21 Dec 2023
The Cousa objective: Having 20 mm of working distance in air can enable new experiments for two-photon imaging, particularly challenging experiments in living animals. The Cousa manuscript is out now. Open access. doi.org/10.1038/s41592-023-0… (1/n)
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Out yesterday from the Yu and Smith labs! The Cousa objective is an ultra-long working distance air objective optimized for 2P and 3P imaging that opens the door to new imaging experiments, including in non-human primates. nature.com/articles/s41592-0…
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The Cousa objective: Having 20 mm of working distance in air can enable new experiments for two-photon imaging, particularly challenging experiments in living animals. The Cousa manuscript is out now. Open access. doi.org/10.1038/s41592-023-0… (1/n)
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Updated preprint for the ultra long working distance Cousa objective. 20 mm air immersion, 2 mm x 2 mm field-of-view, 0.5 NA. biorxiv.org/content/10.1101/… Blog post on it: labrigger.com/blog/2023/07/1…
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Call for Papers through April 1st! "Understanding of Neural Circuits with Neurophotonics" spiedigitallibrary.org/journ…
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More upcoming talks for our February multiphoton imaging workshop (Applications OPEN!) nemonic.ece.ucsb.edu/index.p… Jerome Lecoq @LecoqJerome – "Running a two-photon calcium imaging segmentation pipeline with built-in deep learning denoising at scale"
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Darcy Peterka - "Multiphoton tools for exploring and controlling neural circuits" The multiphoton toolkit has expanded immensely in the last decade. I'll describe some modern tools for imaging & control, with an eye towards considerations for using these tools effectively.
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Johannes Friedrich – “Online methods for real-time analysis of calcium imaging data” We will present online adaptations of the CNMF as well as the CNMF-E algorithm, which dramatically reduces memory and computation requirements. (Plus more topics!)
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Highlighting some software and data sections for our upcoming workshop! Peter Rupprecht: Interpretation of calcium imaging data: post-processing, spike inference and artifacts
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Kushal Kolar: New mesmerize library helps w/data organization & parameter optimization for calcium imaging analysis github.com/nel-lab/mesmerize… "fastplotlib is a new visualization library using bleeding edge graphics technology github.com/kushalkolar/fastp…"
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Applications can be found here: nemonic.ece.ucsb.edu/index.p… Please spread the word and encourage anyone interested in multiphoton imaging to apply!
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Call for papers for a special section on Understanding of Neural Circuits with Neurophotonics is open now! Guest editors include nemonic's own Spencer Smith @Labrigger spiedigitallibrary.org/journ…
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We are very excited with our group of speakers for this year's workshop! This is a hands-on workshop, where you will run imaging analysis software and see instrumentation demonstrations. slslab.org/NWA_2023.pdf
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Apply now to the 2023 Nemonic Workshop! This event will be held Feb 21-23, 2023 in Santa Barbara slslab.org/NWA_2023.pdf. Hear from experts and see demonstrations on multiphoton imaging instrumentation and analysis. Past material: nemonic.ece.ucsb.edu/index.p…
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Other presenters either aren't on Twitter or I haven't located them yet. Materials from prior workshops here: nemonic.ece.ucsb.edu/index.p… (2/2)
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Come to the 2023 Nemonic Workshop. slslab.org/NWA_2023.pdf Feb 21-23, 2023 in Santa Barbara, California. @UNC_Optics @ptrrupprecht @LecoqJerome and others. Come learn about the cutting edge of multiphoton (2p,3p) imaging in neuroscience, both instrumentation and analysis. (1/2)
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