@KosterLab

Interested in electron microscopy in all its flavors, but lately especially in combining cryo electron tomography with artificial intelligence.

 Leiden, The Netherlands
kosterlab.nl
Joined February 2016
  • Tweets176
  • Following181
  • Followers546
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Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles biorxiv.org/cgi/content/shor…
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Today visiting the Francis Crick Institute in London! An amazing place where they perform awesome electron microscopy on such a wide variety of samples!
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napari-animation has had a much needed upgrade! 🥳 The @napari_imaging plugin now includes contributions from 10 people 😍🧑‍🤝‍🧑 Special thanks to @KA_Hutchings, a talented student from @cjrlab this summer, for many significant usability improvements! ⚒️ github.com/napari/napari-ani…
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Bram Koster retweeted
Zhen Chen@ZhenChentccz29 Sep 2022
We optmized in situ cryoET to achieve 6 A reconstructions of mouse sperm doublets! The unique tertiary structures allow de novo protein identification of novel sperm proteins by computationally searching in the AlphaFold2 library of entire mouse proteome! biorxiv.org/content/10.1101/…

De novo protein identification in mammalian sperm using high-resolution in situ cryo-electron...

Understanding molecular mechanisms of cellular pathways requires knowledge of the identities of participating proteins, their cellular localization and their 3D structures. Contemporary workflows...

biorxiv.org
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📢The speaker of the next I2PC Seminar Series on #CryoET will be Jorge Jiménez de la Morena from @CNB_CSIC 📔Talk: ScipionTomo: a cryoET framework. ⏰ Sept29th 15:00 Madrid GMT+2 conectaha.csic.es/b/bla-rkh-…
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This newly published review by eBIC staff discusses recent examples on how cryoET can be used to study membrane remodelling processes. Find the paper here: doi.org/10.1016/j.sbi.2022.1…
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Seeking a postdoc to build super-resolution cryo-fluorescence microscope (#CLEM, #teamtomo) in collaboration with Huang group of #PurdueEngineering. Info and application at engineering.purdue.edu/Engr/…. Please RT!

Correlated super-resolution cryo-fluorescence microscopy and electron microscopy

engineering.purdue.edu
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Correlative light electron microscopy using small gold nanoparticles as single probes. arxiv.org/abs/2209.07771
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HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography biorxiv.org/cgi/content/shor…
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Check out #DeepHEMNMA in #ContinuousFlexPlugin (ver 3.2.0) for @scipion_em: github.com/scipion-em/scipio… pypi.org/project/scipion-em-…

GitHub - scipion-em/scipion-em-continuousflex: Plugin for continuous conformational flexibility...

Plugin for continuous conformational flexibility analysis containing HEMNMA, StructMap, HEMNMA-3D, TomoFlow, NMMD, and DeepHEMNMA for in vitro and in situ cryo-EM/ET. - scipion-em/scipion-em-contin...

github.com
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Bram Koster retweeted
WEBKNOSSOS
@webknossos15 Sep 2022
🐣 New feature 🐣 You can now download datasets in webKnossos using the Python library. Just follow the steps indicated in the UI and integrate it in your own scripts!
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Hello #teamtomo! Have you loved every FIB slice, tomogram & sub volume at #tomo2020? Great news! Andrew Leis & I will be co-chairing #tomo2024 & #tomo2026! We’ll be headed ‘down under’ to Melbourne, AUS in 2024 & ‘up North’ in Madison, WI USA in 2026! #koalas & #Badgers 🐨🦡❄️🔬
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Bram Koster retweeted
Alex Hall@allopatry12 Sep 2022
This recent paper in @Dev_Cell includes Serial Block Face Imaging on a @thermosciEMSpec Apreo Volume Scope followed by segmentation and visualization in Amira Software! Great study, and congrats to the authors.
MPI Molecular Biomedicine@MPI_Muenster23 Aug 2022
New study by Ivan Bedzohv’s team @emselor published in @Dev_Cell identifies Rap1 as a central factor for orchestrating the morphogenetic program that reshapes the epiblast. Congratulations 🎉 to the entire team on this great work!
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How does the yeast nuclear pore complex ‘punch’ a hole in the nuclear envelope to create a functional transport channel? A recent collaborative study from the @KarstenWeisLab implicates the protein Brl1 in this membrane fusion event. More: biol.ethz.ch/en/news-and-eve…
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On my way to #tomo2022! So much looking forward to it! I can't wait to meet old friends and make new ones with those I only know by name from papers or twitter! So many awesome developments! First,  I have to bicycle to catch my train to be on time for the 1st session in 5 hrs.
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Generation 3.3 laser phase plate in the microscope!
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Bram Koster retweeted
Bubeck Lab@BubeckLab6 Sep 2022
Fantastic work by PhD student @EmmaCouves! A real team effort from @scott_d_gardner @TomasVoisin @jasminebickel @pstansfeld @TateScience @imperialcollege @ICL_Receptors
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This week's release includes tomograms of #SARSCoV2 spike-bearing virus-like particles in an experiment which captures various #Covid #Spike #Protein intermediates! EMDB: ebi.ac.uk/emdb/EMD-24601 Paper: doi.org/10.1126/sciadv.abo31…
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No motion correction was applied yet. We further demonstrate effects of the probe size and much more. Manuscript: doi.org/10.1038/s41592-022-0… Raw data on EMPIAR will be available very soon e.g. EMPIAR-11034 4/4
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Bram Koster retweeted
Yanan Zhu@YananZhu75 Sep 2022
nature.com/articles/s41467-0… We determined the structure of pMMO in its native membrane to 4.8 Å by cryoET STA. Our structure revealed lipid-stabilised features and a higher-order hexagonal array arrangement in intact cells. @GroupZhang @RosenzweigLab @OxfordStrubi @eBIC_Diamond

Structure and activity of particulate methane monooxygenase arrays in methanotrophs

Nature Communications - Particulate methane monooxygenase (pMMO) is the main enzyme used by methanotrophs. Here, the authors determined the native structure of pMMO by cryo-electron tomography,...

nature.com
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