@Hollensteinlab

Our group focuses on generating modified aptamers and developing chemoenzymatic methods for the synthesis of chemically modified, therapeutic oligonucleotides

 Paris, France
research.pasteur.fr/en/team/bioorganic-chemistry-of-nucleic-acids/
Joined August 2017
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  • Following1.1K
  • Followers1.3K
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Great news! Moderna cancer vaccine stops melanoma returning: what’s next for personalized treatments? nature.com/articles/d41586-0…

Moderna cancer vaccine stops melanoma returning: what’s next for personalized treatments?

Nature - Promising trial results suggest cancer vaccines work and could be used to target other tumours.

nature.com
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Very happy to share a preprint on our contribution to identifying a polymerase capable of accepting heavily modified nucleotides. Great collaboration with Marc Delarue and @ChaputLab. Congrats to @MariaPozz1! @DBSC_IP @institutpasteur @AgenceRecherche biorxiv.org/content/10.64898…

Polymerase Evolution Enables Access to Glycosylated Xeno-Nucleic Acids with Expanded Chemical...

Chemical modifications expand the functional repertoire of nucleic acids, but the extent to which complex glycans can be integrated into genetic polymers remains largely unexplored. Glycosylated...

biorxiv.org
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Very happy to share our new article published @ChemBioChem on enzymatic GalNAc-labelling of oligonucleotides. Big congrats to @pierrenicolas_b and all authors! chemistry-europe.onlinelibra…

Towards the Enzymatic Labeling of Oligonucleotides With Triantennary N‐Acetyl Galactosamine

Attachment of trivalent GalNAc ligands to short therapeutic oligonucleotides ligands enables receptor-mediated delivery to hepatocytes. Here, we report two complementary chemoenzymatic strategies...

chemistry-europe.onlinelibrary.wiley.com
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Very happy to share our new manuscript @BioconjChem on the enzymatic synthesis of Glyco-DNA Equipped with Oligosaccharides and Charged Monosaccharides. Congrats to @MariaPozz1 and Marc! @institutpasteur @CNRSchimie pubs.acs.org/doi/10.1021/acs…

Enzymatic Synthesis of Glyco-DNA Equipped with Oligosaccharides and Charged Monosaccharides

Abstract. The therapeutic and biotechnological utility of synthetic oligonucleotides is constrained by the limited chemical diversity of canonical nucleoti

pubs.acs.org
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Great visit and lecture at Institut Pasteur thanks to @Hollensteinlab invitation.
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Thrilled to share our recent pre-print where we studied the stereochemical outcome of phosphorothioate bond formation during ligation. Congrats to Erwann and @NazariiSabat. Great collaboration with @Roche. @DBSC_IP @CNRSchimie chemrxiv.org/doi/full/10.264…

Chemoenzymatic Access to Stereodefined Phosphorothioate Oligonucleotides by Stereoselective DNA...

Recent studies have demonstrated that stereopure oligonucleotide phosphorothioates (PS), with defined configuration, provide significant advantages over stereorandom mixtures in therapeutic oligonu...

chemrxiv.org
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Very happy to share our pre-print on the enzymatic synthesis of DNA equipped with short oligosaccharides. Great collaboration with Marc Delarue and Laurence Mulard. Congrats to Maria Dalla Pozza and Marc! chemrxiv.org/doi/full/10.264… @DBSC_IP @institutpasteur

Enzymatic synthesis of glyco-DNA equipped with oligosaccharides and charged monosaccharides |...

The therapeutic and biotechnological utility of synthetic oligonucleotides is constrained by the limited chemical diversity of canonical nucleotides and their susceptibility to nuclease degradation....

chemrxiv.org
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Very happy to share our recent article @ChemBioChem on the synthesis of Ru-complex modified DNA by copper-free click chemistry together with @GasserGroup Congrats to all authors particularly Germain and Alix! @institutpasteur @pierrenicolas_b @CNRS chemistry-europe.onlinelibra…

Incorporation of Ruthenium Polypyridyl Complexes Into DNA Oligonucleotides by Copper‐Free Click...

Alternative methods for the preparation of naïve libraries for SELEX experiments are in dire need, particularly when hydrophobic, bulky, and complex modification patterns are considered. Here, we...

chemistry-europe.onlinelibrary.wiley.com
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Thrilled to share our review article @TrendsChemistry on modified aptamers. Congrats to all authors! Great collaboration with @juliantanner1 . doi.org/10.1016/j.trechm.202…
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Online now: Expanding the chemical repertoire of aptamers dlvr.it/TPfm3R

Expanding the chemical repertoire of aptamers

Aptamers offer distinct advantages over conventional small molecules or antibodies due to their high affinity and specificity. Additional benefits, including small size and ease of modification,...

cell.com
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Hollenstein group retweeted
Gasser Group@GasserGroup12 Nov 2025
Thrilled to share our article just published in @J_A_C_S on the selection of Ru-modified aptamers! Fantastic collaboration with @Hollensteinlab, congratulations to all authors! doi.org/10.1021/jacs.5c13224

Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against...

Abstract. Photodynamic therapy (PDT) harnesses the combination of light, oxygen, and photosensitizers to induce cell death via reactive oxygen species (ROS

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Thrilled to share our article @J_A_C_S on the selection of Ru-modified aptamers! 7 years in the making. Congrats to all authors! Great collaboration with @GasserGroup pubs.acs.org/doi/full/10.102… @GermainNiogret @institutpasteur @pierrenicolas_b

Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against...

Abstract. Photodynamic therapy (PDT) harnesses the combination of light, oxygen, and photosensitizers to induce cell death via reactive oxygen species (ROS

pubs.acs.org
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I'm very happy to announce that our article has just been published online in Communications Chemistry! Thank you @Hollensteinlab for giving me the opportunity to work on this project! 🧬 nature.com/articles/s42004-0…

Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection

Communications Chemistry - Chemical modifications of aptamers can enhance binding properties, however, the design of suitable functional groups remains challenging. Here, the authors introduce...

nature.com
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Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection bit.ly/46lknyH
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Interested by 3D modified aptamers? Then check out our recent article by undergraduate student Charlotte and postdoc Usman. nature.com/articles/s42004-0… @CNRS @institutpasteur @juliantanner1

Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection

Communications Chemistry - Chemical modifications of aptamers can enhance binding properties, however, the design of suitable functional groups remains challenging. Here, the authors introduce...

nature.com
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Very happy to share our preprint @ChemRxiv on the selection of Ru-modified aptamers. Long in the making. Congrats to all authors. Great collaboration with @GasserGroup and @Chrom_Inf @institutpasteur @CNRS go.shr.lc/4lOBL5J

Selection of Ruthenium Polypyridyl Complex-modified Aptamers for Photodynamic Therapy against...

Photodynamic therapy (PDT) harnesses the combination of light, oxygen, and photosensitizers to induce cell death via reactive oxygen species (ROS) formation. Given its intrinsic properties, PDT...

chemrxiv.org
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Very much looking forward presenting our research at #biotrans2025 in Basel. Thanks again for the kind invitation!
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Very happy to share out new pre-print on the enzymatic GalNAc-labelling of oligonucleotides. Big congrats to undergraduate student Elisa and graduate student @pierrenicolas_b ! chemrxiv.org/engage/chemrxiv… @institutpasteur @CNRSchimie

Towards the enzymatic labelling of oligonucleotides with triantennary N-acetyl galactosamine |...

The inclusion of chemical modifications into oligonucleotides stabilizes their backbones against nuclease mediated degradation and improves their biological activity. Even though cellular, tissue,...

chemrxiv.org
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Hollenstein group retweeted
Chemistry Europe@ChemEurope14 May 2025
#ChemBioChem #OnTheCover Recent Advances in Biocatalytic and Chemoenzymatic Synthesis of Oligonucleotides (ChemBioChem 9/2025) (Marcel Hollenstein and co-workers) onlinelibrary.wiley.com/doi/… • onlinelibrary.wiley.com/doi/…
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Hollenstein group retweeted
CNRS Biologie@CNRSbiologie26 Mar 2025
ReNAissance : révolutionner les traitements antiviraux grâce à l’ARN messager 🤝 @CNRSchimie @CNRS_dr17 @CNRS_Meudon 👉 buff.ly/EVQo0Y6
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