cryo-EM structures and methods development, cisTEM development
Madison, WI
Joined July 2021
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Making cryo-EM grids by flying particles through a native mass-spec is pretty cool, but can it be cooler? Yes - by adding a LASER! Particles damaged by the process can be fixed by adding a thin layer of ice then briefly hitting the grid with a laser!
tinyurl.com/49aurm35
Tim Grant retweeted
Learning how to process data using cisTEM and Relion at the cryoEM @cshlcourses ! @GrantCryoEMLab
Tim Grant retweeted
Congrats to @tommy_anderson2 on his first author publication from our lab detailing the first structure of a polymerase complex from an alphacoronavirus, PEDV! Also a big shoutout to Josh Coon who helped with the native MS analysis! academic.oup.com/nar/advance…
Tim Grant retweeted
1/6: #CryoEM Exhausted with fighting the air-water interface (AWI) problem during sample grid plunge freezing?
We may have an easy solution for you!
Read our latest preprint on how we use LEA proteins as AWI sample protectants for challenging samples: biorxiv.org/content/10.1101/…
Great to have Pete in the lab!
Grad student Peter Ducos was a drone pilot for the US Army. Now he helms the controls for equipment used in #cryoEM research to solve complex problems by exploring molecular structures in exquisite detail. morgridge.org/story/rising-s… #RisingSparks #FearlessScience
nature.com/articles/d41586-0…
I feel there can't be many people who have a quote containing colorful language in a journal?
Tim Grant retweeted
Congrats to those from our Morgridge community, @RomaBroadberry from the @GrantCryoEMLab and @kyle_flickinger from the @JasonRCantor Lab! 👏 #FearlessScience #RisingSparks
Congrats to the IPiB students who received poster awards at the IPiB Retreat!
Roma Broadberry (Grant Lab)
Jess Davidson (Simcox Lab)
Kyle Flickinger (Cantor Lab)
Evelyn Okal (Raman Lab)
Phoenix Shepherd (Kirchdoerfer Lab)
More photos from the day: flic.kr/s/aHBqjAVhZi
ALT IPiB student Jess Davidson and Biochemistry Professor James Ntambi talk at Jess' poster during a poster session.
Congratulations @RomaBroadberry! Very proud
Congrats to the IPiB students who received poster awards at the IPiB Retreat!
Roma Broadberry (Grant Lab)
Jess Davidson (Simcox Lab)
Kyle Flickinger (Cantor Lab)
Evelyn Okal (Raman Lab)
Phoenix Shepherd (Kirchdoerfer Lab)
More photos from the day: flic.kr/s/aHBqjAVhZi
ALT IPiB student Jess Davidson and Biochemistry Professor James Ntambi talk at Jess' poster during a poster session.
Tim Grant retweeted
Congrats to the IPiB students who received poster awards at the IPiB Retreat!
Roma Broadberry (Grant Lab)
Jess Davidson (Simcox Lab)
Kyle Flickinger (Cantor Lab)
Evelyn Okal (Raman Lab)
Phoenix Shepherd (Kirchdoerfer Lab)
More photos from the day: flic.kr/s/aHBqjAVhZi
ALT IPiB student Jess Davidson and Biochemistry Professor James Ntambi talk at Jess' poster during a poster session.
Tim Grant retweeted
An Oct. 2-5 workshop @WIDiscovery will bring together #MassSpec and #CryoEM scientists to explore the possibilities and challenges of these novel techniques. Registration is open. For more info, visit coonlabs.com/ms_23/.
@Coon_Labs @erwright73 @GrantCryoEMLab
Tim Grant retweeted
We're #hiring! Join our department as a Professor of Biochemistry (open rank). Visit go.wisc.edu/biochem-faculty-… for more info and to apply.
#FacultyJobs #PhDJobs #Biochemistry #TenureTrack
ALT We're hiring an assistant, associate, or full professor! Apply online by October 15. Link in post. Background shows Biochemical Sciences Building at sunset.
I love it when proteins move a lot! In our just published structure of DNA replication restart proteins PriA/B + replication fork (nature.com/articles/s41467-0…) we see one domain swing almost 90° upon DNA binding, turns out this is important. Awesome collaboration with @kecklabuw.
Tim Grant retweeted
Our latest publication reports the cryo-EM structures of the human Polα-Primase DNA elongation complexes - rdcu.be/c96yY A Lim lab X Tahirov lab collaboration led by @QixiangHe and Andrey. Thank you, @sarakosman & @NatureSMB, for working with us! Please RT to share! 1/4
Tim Grant retweeted
Attend next week's International Steenbock Lectures in Biochemistry to hear from Sjors Scheres @MRC_LMB on developments in #cryoEM and what cryo-EM can tell us about #amyloid filaments in the brain. Livestream: go.wisc.edu/bio_colloq
@SjorsScheres @GrantCryoEMLab
ALT Flier for lectures. May 2nd lecture titled “Cryo-EM structures of amyloid filaments from human brain.” May 3rd lecture titled “Recent developments in cryo-EM image processing.” Both from 3-4 p.m. Reception immediately follows May 2nd lecture. Both held in Room 1211 Biochemical Sciences Building at UW–Madison. Livestream at go.wisc.edu/bio_colloq. For more information contact Scheres’ hosts, Ci Ji Lim and Tim Grant.
Tim Grant retweeted
From Alexis Rohou (@AlexisRohou) of @genentech and colleagues, a video article on how to configure gP2S, a web application for the tracking of cryoEM experiments.
Watch the video on JoVE: hubs.ly/Q013j9xW0
Really cool!
Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes dx.doi.org/10.7554/eLife.726…
Tim Grant retweeted
Delighted that our work with Jamie Williamson and @jrabuckgibbons on characterizing ribosome assembly heterogeneity is out in @Structure_CP. Raw data that gave rise to 42 distinct intermediates is freely available on @EMDB_EMPIAR. More below. 1/12
Tim Grant retweeted
Interested in #teamtomo & how to strategize for #montage & other aspects of #cryoET data collection? Try #TomoGrapher at github.com/wright-cemrc-proj… written by @larsonmattr & @JYang22638056 @UWBiochem. Read about developments in #cryoCLEM #cryoFIB #cryoET at biorxiv.org/cgi/content/shor…
Tim Grant retweeted
Just posted @biorxivpreprint manuscript reporting neutralizing antibody evasion and #CryoEM atomic-level structure of #COVID19 #OmicronVariant spike protein-ACE2 interface (PDB 7T9L), suggesting rationale for increased transmissibility of #Omicron biorxiv.org/content/10.1101/…
