@DouganLab

Regulated proteolysis | N-degron | AAA+ machines | #Proteostasis https://xitter.cf/t.co/8P66UdKcLf https://xitter.cf/t.co/Wa9filzfVh… views my own

 Melbourne, Australia
scholar.google.com/citations?user=zOiG8hsAAAAJ&hl=en
Joined April 2015
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61Media
Multimodal substrate recruitment enables CTLHMKLN1E3 ligase to target acetylated N-, C-, and internal degrons nature.com/articles/s41467-0…

Multimodal substrate recruitment enables CTLHMKLN1 E3 ligase to target acetylated N-, C-, and...

Nature Communications - The CTLHMKLN1 E3 ligase recruits diverse substrates but the underlying mechanisms were unclear. Here, the authors use cryo-EM, biochemistry, and cellular experiments to show...

nature.com
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Dougan Lab retweeted
ShorterLab@ShorterLabOct 18
Mechanistic classification of the AAA superfamily with protein language models biorxiv.org/content/10.64898…

Mechanistic classification of the AAA superfamily with protein language models

The A TPases A ssociated with various cellular A ctivites (AAA) are a class of proteins with diverse structure-function relationships whereby conserved 3-dimensional architecture is employed in...

biorxiv.org
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Read this #preprint on @researchsquare: DPP9 triggers N-degron-mediated clearance of lysosome-escaped cathepsins to safeguard cells researchsquare.com/article/r…

DPP9 triggers N-degron-mediated clearance of lysosome-escaped cathepsins to safeguard cells

Cathepsins, the principle proteases of the endolysosomal compartment, can escape into the cytosol and nucleus following lysosomal membrane permeabilization (LMP). LMP-associated toxicity of nucleo-...

researchsquare.com
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An adaptor for feedback regulation of heme biosynthesis by a mitochondrial protease | Science science.org/doi/10.1126/scie…

An adaptor for feedback regulation of heme biosynthesis by a mitochondrial protease

Heme biosynthesis is tightly coordinated to support essential functions without accumulating toxic porphyrins and depleting cellular iron. Heme induces degradation of the heme biosynthetic enzyme,...

science.org
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Dougan Lab retweeted
ShorterLab@ShorterLabJul 28
Methionine oxidation alters both helical assembly and disordered contacts in human TDP-43 C-terminal domain phase separation | PNAS pnas.org/doi/10.1073/pnas.25…

Methionine oxidation alters both helical assembly and disordered contacts in human TDP-43 C-termi...

TAR DNA binding protein 43 (TDP-43), a key protein linked to ALS pathology, undergoes phase separation and forms functional assemblies via condensa...

pnas.org
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GID4-Recruiting PROTACs for BRD4 Degradation Overcome Resistance Driven by CRBN and VHL Deficiency | Journal of Medicinal Chemistry pubs.acs.org/doi/10.1021/acs…

GID4-Recruiting PROTACs for BRD4 Degradation Overcome Resistance Driven by CRBN and VHL Deficiency

Abstract. Despite the identification of over 600 E3 ligases, current proteolysis-targeting chimeras (PROTACs) predominantly rely on CRBN and VHL. Recently,

pubs.acs.org
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Structural proteomics reveals that misfolded nascent proteins expose buried lysines for ubiquitination and rapid proteasomal degradation sciencedirect.com/science/ar…
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Overturn this. 🧏‍♂️ #USABEL
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Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex | Nature Communications nature.com/articles/s41467-0…

Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity...

Nature Communications - Transcriptional regulation is a central mechanism underlying bacterial adaptation to stress. Here, the authors used a genetic fitness screen to show that the Y-complex in...

nature.com
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Online Now: Design principles of a membrane-spanning ubiquitin ligase dlvr.it/TSkgS6
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A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation | PNAS pnas.org/doi/10.1073/pnas.26…

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short-...

The Arg/N-degron pathway of Saccharomyces cerevisiae is mediated by two interacting E3 ubiquitin ligases, Ubr1 and Ufd4. We show here that the mito...

pnas.org
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Molecular mechanisms of mitochondrial AAA+ proteases - Journal of Biological Chemistry jbc.org/article/S0021-9258%2…

Molecular mechanisms of mitochondrial AAA+ proteases

Mitochondrial AAA+ proteases, LONP1, ClpXP, YME1L (i-AAA), and the m-AAA complex, maintain protein quality and shape organelle function. Growing interest in these enzymes stems from their association...

jbc.org
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Chemical activation of mitophagy via the N-degron pathway alleviates mitochondrial neuropathies biorxiv.org/content/10.64898…
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Data-Driven Design of PROTAC Linkers to Improve PROTAC Cell Membrane Permeability | JACS Au pubs.acs.org/doi/10.1021/jac…

Data-Driven Design of PROTAC Linkers to Improve PROTAC Cell Membrane Permeability

Abstract. Proteolysis-targeting chimeras (PROTACs) are promising next-generation therapeutics for the degradation of disease-associated proteins. However,

pubs.acs.org
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Molecular mechanisms of mitochondrial AAA+ proteases - Journal of Biological Chemistry jbc.org/article/S0021-9258%2…

Molecular mechanisms of mitochondrial AAA+ proteases

Mitochondrial AAA+ proteases, LONP1, ClpXP, YME1L (i-AAA), and the m-AAA complex, maintain protein quality and shape organelle function. Growing interest in these enzymes stems from their association...

jbc.org
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Our recent work on mechanism of disassembly and reassembly of oligomeric proteins is now published in ACS Biochemistry. doi.org/10.1021/acs.biochem.…

Concerted Deoligomerization and Nonfunctional Reassembly of the Hexameric Proteasomal ATPase Mpa...

Abstract. Mycobacterium tuberculosis Mpa is an AAA+ ATPase that unfolds and translocates substrate proteins during proteasomal degradation. Mpa spontaneous

pubs.acs.org
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CLIP-LUX: A Luminescent Split-Ubiquitin Assay Using ClpS-Mediated N-Degron Recognition for Detection of Protein-Protein Interactions in Mammalian Cells. sciencedirect.com/science/ar…
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Elongation Factor Tu Acts as a Chaperone to Activate an Antibacterial RNase Toxin - goo.gl/scholar/WFsfuJ #ScholarAlerts
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Online Now: Mitochondria as sources and targets of cellular signaling dlvr.it/TQcy31
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Dougan Lab retweeted
ShorterLab@ShorterLabJan 27
Metabolically regulated proteasome supramolecular organization in situ: Cell cell.com/cell/fulltext/S0092…

Metabolically regulated proteasome supramolecular organization in situ

Structural steps along the assembly of proteasome storage granules—membraneless organelles that form in response to metabolic shifts in yeast—are visualized inside cells by cryo-electron tomography....

cell.com
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