@ChanWingFuk

Postdoc @ACBDresearch @MonashUni | Haematopoiesis | Molecular immunology | 3D genome | Gene regulation | CRISPR | Former @WEHI_research

 Melbourne, Victoria
Joined September 2019
  • Tweets242
  • Following116
  • Followers77
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Still feeling it isn't real ... But thrilled that I got another @NatureComms paper with @rhyscienceallan ! A more robust CRISPR activation system TETact in activating transcription from CpG-methylated loci. doi.org/10.1038/s41467-022-3…

Activation of stably silenced genes by recruitment of a synthetic de-methylating module

Nature Communications - Stably silenced genes with methylated CpG at the promoter are refractory to current CRISPR activation systems. Here the authors create a more robust activation system,...

nature.com
Rhys Allan@rhyscienceallan23 Sep 2022
💥💥Excited that our TETact study has now been published in @NatureComms nature.com/articles/s41467-0… @ChanWingFuk did a huge amount of new work in response to reviewers comments (e.g. multiple cell types, multiple genes activated in the same cell)

Activation of stably silenced genes by recruitment of a synthetic de-methylating module

Nature Communications - Stably silenced genes with methylated CpG at the promoter are refractory to current CRISPR activation systems. Here the authors create a more robust activation system,...

nature.com
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Transcription-coupled AID deamination damage depends on ELOF1-associated RNA polymerase II dlvr.it/TJy7Wq
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The Nobel Prize
@NobelPrize7 Oct 2024
BREAKING NEWS The 2024 #NobelPrize in Physiology or Medicine has been awarded to Victor Ambros and Gary Ruvkun for the discovery of microRNA and its role in post-transcriptional gene regulation.
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Jacob Corn@jcornlab13 Aug 2024
Having a hard time with #CRISPR HDR? Your cells might be fighting against you! TREX1 nuclease, high in some cells, eats up HDR donor templates. Check out our paper in @NatureBiotech for the story & ways to improve HDR. nature.com/articles/s41587-0… With @karasuerman1 & @J_Maciejowski
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I'm a little late to the party, but glad to share that our manuscript describing click editing has been published in @NatureBiotech. An amazing team effort led by @FerreiraSilvaJ @connorjtou & others. @CGM_MGH @MGH_RI @harvardmed #clickediting 🧬 nature.com/articles/s41587-0…

Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases

Nature Biotechnology - Click editing uses DNA polymerases, HUH endonucleases and oligonucleotide templates for genome modification.

nature.com
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David R. Liu
@davidrliu10 Jun 2024
Today we report in @natBME the eePASSIGE system, which uses evolved and engineered recombinases and prime editing to integrate large gene-sized DNA cargoes into the mammalian genome in an efficient, precise, and targeted manner. (1/13) drive.google.com/file/d/1WDM…
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😀😀Excited to share our new paper published @genomeresearch ! Its been a long journey and huge effort from many @WEHI_research colleagues in particular @keeeeeeeeeeenan and @HDCoughlan who studied the importance of heterochromatin for 3D genome organisation👇
WEHI (Walter and Eliza Hall Institute)@WEHI_research22 May 2024
WEHI researchers have revealed that repairing disordered heterochromatin might aid in healthy #immune ageing. Loss of heterochromatin affects protein-making gene activity in specific areas of the cell nucleus. Published in Genome Research: m.genome.cshlp.org/content/3… (242) 🧵1/2
A 3D computer reconstruction showing changes in the nucleus in control cells (left) and in Suv39h knockout cells (right). Suv39h knockout cells lose the blue and purple beads, which are chromatin domains that are normally tethered to the outside of the nucleus in the control cells.

ALT A 3D computer reconstruction showing changes in the nucleus in control cells (left) and in Suv39h knockout cells (right). Suv39h knockout cells lose the blue and purple beads, which are chromatin domains that are normally tethered to the outside of the nucleus in the control cells.

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Prime editing using CRISPR-Cas12a and circular RNAs in human cells go.nature.com/47u2tIO
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A base editor is engineered for A-to-T and A-to-C transversion editing go.nature.com/3Cwgjh6
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David R. Liu
@davidrliu8 Jan 2024
Today we report in @NatureBiotech an engineered virus-like particle system for in vivo delivery of prime editor protein-RNA complexes. This PE-eVLP platform can support therapeutic prime editing in animal models of genetic disease. /1 drive.google.com/file/d/1xd7…
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Out now in @PLOSBiology 👇 TLDR: Transcription CAN directly remodel TADs... in specific contexts. Big congrats to Sanjay and @yousra_zouari who spearheaded the project, and @djost_physbiol +Hossein journals.plos.org/plosbiolog…

Transcription induces context-dependent remodeling of chromatin architecture during differentiation

Links between genome organization and transcriptional control have been controversial and unclear. Although most TADs (topologically associated domains) are stable through development, this study...

journals.plos.org
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Sternberg Lab@SternbergLab1 Dec 2023
New pre-print from the Sternberg Lab! Transposon-encoded nucleases (TnpB/IscB) gave rise to the CRISPR-associated proteins (Cas9 and Cas12), but were TnpB-like proteins domesticated for other functions? 𝙔𝙀𝙎!! doi.org/10.1101/2023.11.30.5…
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Our #epigenetic atlas of the #mammarygland, was published yesterday. This work has highlighted how little we knew about gene regulation in normal breast cells and how much we don’t understand about #cancer formation. #cellgenomics #wehi_research cell.com/cell-genomics/fullt…

Three-dimensional genome architecture coordinates key regulators of lineage specification in...

Milevskiy et al. investigate the epigenetic states of three epithelial lineages and two stem cell populations in the mouse mammary gland. Each epithelial cell type utilizes distinct epigenetic...

cell.com
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G-quadruplexes associated with R-loops promote CTCF binding dlvr.it/SvnGWT
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David R. Liu
@davidrliu31 Aug 2023
Today we report in @CellCellPress a suite of engineered and PACE-evolved prime editor variants PE6a-g. These new prime editors improve the activity, in vivo deliverability, and in vivo editing efficiency of prime editing. sciencedirect.com/science/ar… PDF:drive.google.com/file/d/1195… (1/15)
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Compact genome editors were discovered by mining a transposon library go.nature.com/3CTqoEH

Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature...

Nature Biotechnology - Compact genome editors were discovered by mining a transposon library.

nature.com
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Online Now: G-quadruplexes associated with R-loops promote CTCF binding dlvr.it/StMCsd

G-quadruplexes associated with R-loops promote CTCF binding

CTCF is a highly conserved protein that is essential for genome organization. Although CTCF recognizes specific DNA sequences, it does not bind to all its potential sites. Wulfridge et al. report...

cell.com
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High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition go.nature.com/43QCv0d
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Online Now: CTCF and R-loops are boundaries of cohesin-mediated DNA looping dlvr.it/St5qZN
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Truely grateful to receive the Emma Whitelaw ECR award ! Thank you @AEpiA for the recognition of my work !
Australasian Epigenetics Alliance@AEpiA19 Jul 2023
✨ ANNOUNCEMENT ✨ Congratulations to @nataliabenetti_ for winning 2nd place, and @ChanWingFuk for winning 3rd place for the Emma Whitelaw ECR Publication Award.
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David R. Liu
@davidrliu3 Jul 2023
Today we report in @NatureGenet a study led by the team of @JonathanYenPhD and Mitch Weiss @StJude that characterizes Cas9 nuclease and adenine base editing strategies to induce fetal hemoglobin expression, clinical approaches to treat sickle-cell disease & beta-thalassemia. 1/10
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