@BoelLab

Our principal interest is to understand the fundamental aspect of protein synthesis mechanisms and regulations.

 @ibpcF Paris, France
ibpc.fr/UMR8261/AccueilEN/Theme%20GB/Theme.html
Joined February 2019
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🚨👨‍🔬👩‍🔬 🚨 We are hiring: a 3-year postdoctoral position to study ABCF proteins in cyanobacteria. @IbpcF @EgmIbpc #postdocs #Paris #cyanobacteria #ribosome #ABCF
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Looks super cool, can’t wait to try it
Sergej Djuranovic
@DjuranovicLab
22 Oct 2024
Collaborative work of @slavicaPD, @Hashemyaser4, Jovanovic Lab and @DjuranovicLab - a new method to isolate ribosomes and translational material in minutes. We show multiple usage of our RAPPL method in biochemical and structural assays with ribosomes. Please be free to contact us for further instructions if needed. @IsraelF96135088 you'll like this one. A rapid, facile, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies biorxiv.org/content/10.1101/…

A rapid, facile, and economical method for the isolation of ribosomes and translational machinery...

Short abstract Ribosomes are essential RNA-protein complexes involved in protein synthesis and quality control. Traditional methods for ribosome isolation are labor intensive, expensive, and require...

biorxiv.org
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Our timely contribution to the growing body of YebC2/TACO1 literature 🤩! YebC2 is a third factor that prevents ribosome stalling at prolines. Associates with 70S and important for fitness in absence of EF-P and YfmR. biorxiv.org/content/10.1101/…

YebC2 resolves ribosome stalling at polyprolines independent of EF-P and the ABCF ATPase YfmR

Polyproline motifs are essential structural features of many proteins, and recent evidence suggests that EF-P is one of several factors that facilitate their translation. For example, YfmR was...

biorxiv.org
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Activity, structure, and diversity of Type II proline-rich antimicrobial peptides from insects | EMBO reports embopress.org/doi/full/10.10…

Activity, structure, and diversity of Type II proline-rich antimicrobial peptides from insects

EMBO Reports - Apidaecin 1b (Api), the first characterized Type II Proline-rich antimicrobial peptide (PrAMP), is encoded in the honey bee genome. It inhibits bacterial growth by binding in the...

link.springer.com
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This is really a stunning paper. Cyanobacteria exposed to shorter days (in the lab) "rewire" their membrane lipids to anticipate cold weather. When those cells are plunged into cold water, they have "two or three-times higher survival" than cells exposed to longer days.
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Our collaboration with @WilsonLab2 and @vpelech is out in @NAR_Open! The ABCF ATPase New1 resolves translation termination defects associated with specific tRNAArg and tRNALys isoacceptors in the P site academic.oup.com/nar/advance…

The ABCF ATPase New1 resolves translation termination defects associated with specific tRNAArg and...

Abstract. The efficiency of translation termination is determined by the nature of the stop codon as well as its context. In eukaryotes, recognition of the

academic.oup.com
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Congrats to @biozentrum @snsf_ch Ambizione Group Leader @FlorentWaltz on this stunning study🤩 of respirasome structure & organization inside native cells! It's amazing how quickly in situ #cryoET is advancing. Read Florent's thread🧵 for the high-resolution details🔎🦠 #TeamTomo
Florent Waltz@FlorentWaltz
5 Sep 2024
Super excited to share our latest work on the native in-cell organization of the mitochondrial respiratory chain 🥳 Using cryo-electron tomography🔬, we show how the respiratory complexes (and other complexes) are organized inside native mitochondria!  biorxiv.org/content/10.1101/…
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Principal Investigator @gagnon_lab and researchers at @utmbhealth use cryogenic-electron microscopy to elucidate the mechanism of GDP dissociation from RF3 catalyzed by the Escherichia coli 70S-TC. buff.ly/4dxEqwm
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Tour-de-force by @Jonatha31791089 enabling visualization of protein folding within cells by cryo-ET. Great collaboration with @MPI_Biochem colleagues, just out @Nature rdcu.be/dRAQ8
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Here, Koch lab reports on bacterial ribosome hibernation. They show that the membrane-tethering hibernation factors YqjD/ElaB/YgaM, discovered by Wada in E. coli, are widely conserved in proteobacteria and likely target the ribosomal peptide tunnel👍 biorxiv.org/content/10.1101/…
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Very happy to announce that our paper “Global regulation via modulation of ribosome pausing by the ABC-F protein EttA” has been published nature.com/articles/s41467-0…. Congratulation to all the lab members and especially the first authors @ousalemF @CNRS @IBPCF @univ_paris_cite @EGM

Global regulation via modulation of ribosome pausing by the ABC-F protein EttA

Nature Communications - The physiological function of the ABC-F translation factor has remained elusive. Here, the authors show that one of them, EttA, regulates the translation of mRNAs encoding...

nature.com
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EttA-dependent regulation can be reversed—induced in the absence of EttA and repressed in its presence—by the use of a leader peptide like MgtL.
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We designed a motif that makes the expression of YFP strongly EttA-dependent. This motif demonstrates that EttA's activity is only evident when the motif is present within the first 30 amino acids of the encoded sequence.
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Out now in @PNASNews we used Tn-seq to identify genetic interactions with EF-P in B. subtilis. We found that 1) the ABCF YfmR (Uup) is required in absence of EF-P in some conditions pnas.org/doi/10.1073/pnas.23…
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Bacterial housekeeping ABCFs are tricky targets. What do they do in the cell? Which ribosomal stalls do they resolve? This is our take on the subject in Bacillus subtilis. biorxiv.org/content/10.1101/…

Resolution of ribosomal stalling by ABCF ATPases YfmR and YkpA/YbiT

Efficiency of protein synthesis on the ribosome is strongly affected by the amino acid composition of the assembled amino acid chain. Challenging sequences include proline-rich motifs as well as...

biorxiv.org
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The paper with our wonderful collaborators @WislonLab2 and @gem_atkinson on the Pth-catalyzed nascent chain release is out. cell.com/molecular-cell/full…

Peptidyl-tRNA hydrolase is the nascent chain release factor in bacterial ribosome-associated...

Svetlov et al. show that, following ribosome splitting, peptidyl-tRNA that remains stuck inside the large ribosomal subunit is released by peptidyl-tRNA hydrolase (Pth). This release requires the...

cell.com
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My first review with @InnisLab and @ThibaudRenault is now out! Thank you to the team for the enriching and fun times writing and discussing 😄 Happy reading this new year!
good papers@paperperday
31 Dec 2023
The ribosome as a small-molecule sensor sciencedirect.com/science/ar…
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