@BoHuangLab
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Watching the inner life of a cell
San Francisco, CA
Joined July 2019
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New in @CellCellPress from Biohub Investigator @BoHuangLab: AI-designed proteins built from scratch (dubbed "Rhobins") work in living cells and match or even surpass fluorescent tags refined over decades of natural protein engineering.
Read the paper: cell.com/cell/fulltext/S0092…
Bo Huang Lab retweeted
What if pooled screens could measure dynamic cell behavior in real time?
RainBar uses fluorescent protein barcodes and cytoplasmic marker mediated segmentation to perform optical pooled screening in live cells.
Preprint: biorxiv.org/content/10.1101/…
ALT Highly accurate clustering of cells by RainBar barcode. Each cluster represents a unique fluorescent protein barcode identity used in optical pooled screening.
ALT Pooled cells expressing RainBar fluorescent barcodes imaged together in a single field during live cell imaging.
HybriSeq: our venture into single-cell RNA profiling by combing in situ hybridization and single-cell sequencing. doi.org/10.1101/2023.09.27.5…
CELL-E 2 is ready to transform protein sequences to images as well as generate sequences from images. bohuanglab.github.io/CELL-E_…
Endogenous tagging for structural biology pnas.org/doi/10.1073/pnas.22…
Here is our first step towards predicting cellular activities of proteins from their amino-acid sequences using deep-learning: biorxiv.org/content/10.1101/…
The OpenCell is now open! doi.org/10.1126/science.abi6…
A tribute to team science! @labs_mann @czbiohub @kchev @ADBxMPIB @preethiragh @loicaroyer @marcoyannic @mattersOfLight @JswLab @BoHuangLab @liilii_tweet @heracanaj @yeaarachelll + many other authors. So much fun to work with you all.
Bo Huang Lab retweeted
LR-ExSIM and LR-ExSTORM reveal subcellular protein organization. @XiaoyuShi2, Qi Li and colleagues report the development of label-retention expansion microscopy (LR-ExM): bit.ly/3qR6vXX
@IanSeiple @BoHuangLab @jiapeichen @dhivkmr @ReiterLab @ExMicroscopy
#Technology
LR-ExM and eSPIM also helped resolving endosome structures and dynamics in zebrafish embryo.
We are so happy to help Su Guo lab at UCSF use our new method LR-ExM to visualize Par-3 assisted polarized endosome dynamics in zebrafish. pharm.ucsf.edu/guo, @BoHuangLab, @IanSeiple . advances.sciencemag.org/cont…
Thanks to @ReiterLab's cool work. LR-ExM is already helping discoveries in centrosome/ciliary structures.
Dhivya Kumar @dhivkmr, @ReiterLab and colleagues identify a multi-protein complex called DISCO (DIStal centriole COmplex) that is essential for the initiation of cilium assembly. bit.ly/3dYZ3Vs
#cilia #centriole #centrosome #cellbiology #biochemistry #cytoskeleton
Expansion microscopy gets help fron new anchors
.@XiaoyuShi2, Li, @IanSeiple @BoHuangLab et al. report the development of label-retention expansion #microscopy w/ a set of trifunctional anchors that not only prevent fluorescent signal loss but also enable high-efficiency labeling using SNAP & CLIP tags. bit.ly/3qR6vXX
Tagging and purification of endogenous proteins for single particle cryo-EM. Nice collaboration with @yifan_ucsf ! biorxiv.org/content/10.1101/…
Just coming out in @CellCellPress: authors.elsevier.com/sd/arti…. Our incredible collaboration with the
@BivonaLabUCSF and @TulpuleLab shows how cytoplasmic protein granules formed by a variety of oncogenic kinase fusion mutants can activate RAS/MAPK signaling.
Bo Huang Lab retweeted
Endogenous tagging + 3D live-cell imaging + mass-spec interactome = opencell.czbiohub.org, an open-source map of the human proteome. So excited for our first release, wonderful collaboration with @labs_mann @loicaroyer & many other colleagues. @OpenCellCZB
OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization biorxiv.org/cgi/content/shor… #bioRxiv
Transfer learning solves the challenge of training data collection for supervised learning and also improves the performance of self-supervised learning. For image denoising, see bioRxiv dx.doi.org/10.1101/2021.02.0… and GitHub github.com/BoHuangLab/Transf…
Here is a truly versatile and modular system for split enzymes, as demonstrated for split HaloTag: disq.us/t/3t8m5zq
Still more to be done, but here is the 3rd-gen of green-colored split fluorescent protein for protein tagging and signal amplification biorxiv.org/cgi/content/shor…
Bo Huang Lab retweeted
It takes a village: great collaboration between @czbiohub @JswLab @BoHuangLab Barbara Panning @LiGanLab - thanks to all.
The bright gets brighter: Split red fluorescent proteins for the visualization of endogenous proteins and synapses disq.us/t/3ibmj76