Scientist in the Hsu Lab, Arc Institute
Joined March 2021
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Alison Fanton retweeted
Excited to share our new preprint!
How do animals transform internal representations into step-by-step navigation to goals? Decoding these strategies directly from behavior is challenging because these internal states are not directly observable.
biorxiv.org/content/10.1101/…
🧵1/n
Thrilled to share our paper, now out in Nature Biotechnology! We engineered recombinases to enable efficient, specific genome insertion of multi-kb DNA cargoes at an endogenous locus. Grateful to all my amazing co-authors!
A study published today in @NatureBiotech from the @pdhsu lab presents a new way to insert large DNA sequences into the genome using engineered recombinases that don’t require DNA cutting or rely on the cell's repair machinery.
🧬 Thrilled to share our latest work on engineering precise DNA recombinases for targeted genome integration! Through directed evolution & protein fusions, we achieved over 50% efficiency & 97% specificity for multi-kb DNA cargo insertions. Big thanks to all co-authors -
especially @BartieLiam and @pdhsu , for your tireless teamwork and support. It’s been a fun PhD!👩🏻🔬
Alison Fanton retweeted
@cyrus_tau and I are so excited to share our work on developing a CRISPR protein-guided, trans-splicing system for making versatile RNA edits!!! See the highlights in Cyrus' thread and the preprint for details!
.@sitaritapita and I are excited to share RESPLICE, a new RNA-programmable mode of editing that leverages RNA targeting CRISPR proteins and the endogenous spliceosomal machinery to enable versatile RNA edits in human cells! (1/12)
biorxiv.org/content/10.1101/…
Alison Fanton retweeted
Amazing week @KeystoneSymp ! So much fun to finally share our work. It’s been an awesome scientific journey characterizing this system with @mgdurrant and our team
Some notes on our bridge recombination system from our preprint 🧵:
Just shared at @KeystoneSymp a new @ArcInstitute discovery of the bridge RNA recombinase mechanism: a new class of natural RNA-guided systems that retains the key property of programmability from RNAi and CRISPR while enabling large-scale genome design beyond RNA and DNA cuts
Alison Fanton retweeted
By the way, if this flavor of molecular technology development excites you, we are hiring (at all levels)! Please reach out 👩💻🧑💻👩🔬👨🏻🔬
Alison Fanton retweeted
The ability of molecular biologists to control DNA has relied on enzymes derived from the phage-microbe arms race. In this new work, we systematically mined thousands of new serine integrases (LSRs) and developed them for human genome editing. 🧬
biorxiv.org/content/10.1101/…