@thejenjay

Postdoc @VollumInstitute studying synapses. Likes cycling, hiking, wrangling data, and petting dogs. Follow me for updates on my plants. She/her.

jennifer-jahncke.netlify.app/
Joined June 2009
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Jennifer Jahncke retweeted
The Onion
@TheOnion16 Dec 2024
‘No Way To Prevent This,’ Says Only Nation Where This Regularly Happens theonion.com/no-way-to-preve…
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The second half of my dissertation work is now up on bioRxiv! Here we examine the contribution of the functional domains of Dystroglycan to inhibitory MLI:PC synapses in cerebellar cortex 🧵
bioRxiv Neuroscience@biorxiv_neursci30 Aug 2024
Distinct functional domains of Dystroglycan regulate inhibitory synapse formation and maintenance in cerebellar Purkinje cells biorxiv.org/cgi/content/shor… #biorxiv_neursci
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What about intracellular interactions? I generated a PC specific Dystroglycan ICD truncation to get at this question. Interestingly, these mice had impaired synapse function but totally normal synapse formation.
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In summary: - Dag1 glycosylation = formation + function - Dag1 ICD = function We think that extracellular interactions likely with a Nrxn family member is supporting synapse structure whereas indirect intracellular interactions are supporting synapse function.
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Do you study cerebellar Purkinje cells? Do you use conditional genetics to manipulate said Purkinje cells? Friend, do I have a paper for you: Tools for Cre-Mediated Conditional Deletion of Floxed Alleles from Developing Cerebellar Purkinje Cells eneuro.org/content/11/6/ENEU…

Tools for Cre-Mediated Conditional Deletion of Floxed Alleles from Developing Cerebellar Purkinje...

The Cre-lox system is an indispensable tool in neuroscience research for targeting gene deletions to specific cellular populations. Here we assess the utility of several transgenic Cre lines, along...

eneuro.org
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For kicks, I also characterized the timecourse of reporter expression and Dystroglycan deletion using virally expressed Cre (AAV8-CMV-Cre at P0) and found a similar timecourse to that of Pcp2-Cre.
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A couple important lessons here. (1) Characterize your Cre line! It might not do what you think it does. (2) Reporter expression ≠ target gene deletion.
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It's hard to believe, but the first of the year's Advanced Research Training Courses is already over! Students in the Analytical and Quantitative Light Microscopy (@AQLMMBL) course arrived on campus on April 24 and head home today.
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