ALT Graphical Abstract contains the following elements: Top text: Australian Genomic Mitochondrial Flagship text flowchart reads 140 individuals with suspected mitochondrial disease --> GS/ES+mtDNA sequencing --> Diagnostic yield Left hand circle beside clip art of an infant and text reading "Pediatric onset" reads 71% with an arrow pointing down at mtDNA next to an indicator reading 22%, and nuclear DNA next to an indicator reading 78% Right hand circle beside clip art of an adult and text reading "Adult onset" reads 31% with an arrow pointing down at mtDNA next to an indicator reading 29%, and nuclear DNA next to an indicator reading 71%