The RISE-GHS training continues with a hands-on Multiplex PCR session, where participants amplify the cDNA prepared during the previous lab session to generate sufficient material for downstream processes. Today's session also covers amplicon clean-up and quality control (QC), helping participants assess the quality of their amplified products before proceeding.

Participants move into the laboratory for a hands-on session on cDNA preparation, converting RNA into complementary DNA (cDNA) as part of the sequencing workflow. They also receive their group allocations ahead of the practical laboratory sessions planned for the rest of the week.

The training continues with Dzidzor Ayeke’s session on best practices for sample handling and quality control in Next-Generation Sequencing. Participants learn that the quality of inputs directly affects sequencing outcomes, with emphasis on proper sample storage, maintaining the cold chain, avoiding repeated freeze-thaw cycles, and accurate quality assessment using tools such as Qubit and TapeStation. The session also highlights the importance of documenting QC metrics and troubleshooting samples that do not meet quality standards before proceeding with sequencing.

The RISE-GHS Ghana training workshop on Next-Generation Sequencing (NGS) and Bioinformatics is underway, bringing together public health laboratory staff to strengthen skills in pathogen genomic surveillance. Prof. Peter Quashie welcomes participants to the five-day training, which focuses on building practical competencies in NGS, bioinformatics and genomic surveillance. Festus Kofi Sroda, Head of Clinical Laboratory Unit of the Ghana Health Service also provides an introduction to the workshop and its relevance to strengthening Ghana’s public health laboratory systems.