New paper alert! 🔗nature.com/articles/s44172-0… Researchers present a cost-effective way to convert a standard commercial microscope, present in many imaging facilities, into a super-resolution microscopy capable of nanoscale imaging via #DNAPAINT #microscopy. Congrats to all authors!!

🔬 New preprint alert 🔬 Authors introduce a method for fast 3D volumetric imaging of whole cells and tissues with DNA-PAINT for nanoscale resolution, no post-processing stitching required. biorxiv.org/content/10.64898… Our anti-GFP, anti-TagFP, and anti-Halo kits were used for DNA-PAINT imaging of nuclear pores, E-Cadherin, and gliotactin in U2OS cells and Drosophila tissue. Congratulations to all authors! #MassivePhotonics #SMLM #Microscopy #DNAPAINT

A new DNA-PAINT protocol just dropped! cell.com/star-protocols/full… Published in STAR Protocols, researchers created a step-by-step guide for fixing and staining Drosophila melanogaster embryos for DNA-PAINT imaging of E-cadherin nanoclusters and F-actin organization at cell-cell junctions at the nanoscale. We're proud that the team recommends our #DNAPAINT ready anti-GFP single-domain antibody (sdAb) as part of their workflow. Congratulations to all researchers!

Not all DNA is created equal and in super-resolution imaging, handedness matters. Left-handed DNA (Z-DNA) reduces imaging background because it's structurally distinct from endogenous B-DNA, which is especially useful for nuclear imaging. Regarding multi-target imaging, swapping your existing docking strand for a left-handed version is an easy way to increase target multiplexing capabilities. Right-handed DNA (B-DNA) is the classic. It's suitable for most targets and the backbone of our #DNAPAINT kits. We now offer both as custom DNA conjugates with your protein of choice. 🔗 Learn more: massive-photonics.com/dna-pa… #MassivePhotonics #SMLM

What does the team at Massive Photonics get up to outside the lab? Next in our series is Jonas Fischer, an avid swimmer. When he's not immersed in code and data, he's immersed in open water. 🏊‍♂️

What does the team at Massive Photonics get up to outside the lab? Next in our series is Angel Cereceda, a running aficionado. When he's not running experiments at the nanoscale, he's running miles at the macroscale. #MassivePhotonics #DNAPAINT #SMLM

🔬New publication spotlight! 🔗:pnas.org/doi/10.1073/pnas.26… While antigen presentation is central to cytotoxic T-cell immunity, how antigens are presented at the nanoscale remains poorly understood. Jacobs, Menche et al. tackled this question in human dendritic cells using quantitative DNA-PAINT microscopy. Their key finding: peptide–MHC I complexes are overwhelmingly monomeric, with antigen presentation regulated by surface density and spatial compartmentalization rather than higher-order clustering. This reframes how we think about T cell triggering and has consequences for the design of cancer vaccines and immunotherapies. Our custom DNA-PAINT anti-ALFA single-domain antibody was used to label peptide–MHC I. #DNAPAINT #SMLM #MassivePhotonics

We made it to #SMLMS2026! Looking forward to a fantastic conference. 🔬 Come find us at our booth to chat #SMLM and #DNAPAINT of course.

What does the team at Massive Photonics get up to outside the lab? Next in our series is Lucia Wunderlich, a fierce rock climber. When she's not exploring cellular targets at the nanoscale, she's scaling mountains. 🏔️

We're heading to Stockholm! 🇸🇪 The Massive Photonics team will be at SMLMS 2026, one of our favorite conferences to connect with the single-molecule localization microscopy community. If you're attending and want to chat microscopy, DNA-PAINT, or just what's new at Massive Photonics, come find us at our booth! #SMLMS2026 #SMLM #SuperResolution #DNAPAINT #Microscopy

What does the team at Massive Photonics get up to outside the lab? We're starting a little series to find out. Kicking things off with Florian Selbach, an avid mountaineer. When he's not putting DNA-PAINT on the map, prefers to go off-map entirely. 🏔️

The diffraction limit stops here. 250 nm resolution blurs away the biology you're trying to study. DNA-PAINT pushes resolution to ~15 nm. The same targets, resolved at nanoscale. What do you want to image at the nanoscale? #DNAPAINT #SuperResolution #Microscopy #SMLM

🌟 New preprint alert 🌟 Stojcic et al. used 3D MINFLUX DNA-PAINT to map Na,K-ATPase clustering with sub-10 nm localization precision directly in mammalian cells. They found a nearest-neighbour distance of ~7 nm, providing direct visual confirmation of Na,K-ATPase dimerization and observed higher-order nanoclusters of up to 21 proteins. Great work! 🔗biorxiv.org/content/10.64898… Our MASSIVE-TAG-X2 sdAb anti-GFP kit was used to label EGFP-tagged Na,K-ATPase subunits. #DNAPAINT #MINFLUX #Microscopy

🚀 𝗔𝗹𝗿𝗲𝗮𝗱𝘆 𝘄𝗼𝗿𝗸𝗶𝗻𝗴 𝘄𝗶𝘁𝗵 (𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁) 𝗽𝗿𝗼𝘁𝗲𝗶𝗻-𝘁𝗮𝗴𝘀? Take your imaging to the nanoscale with our 𝗽𝗹𝘂𝗴-𝗮𝗻𝗱-𝗽𝗹𝗮𝘆 𝗗𝗡𝗔-𝗣𝗔𝗜𝗡𝗧 𝗸𝗶𝘁𝘀 - designed to fit seamlessly into your existing immunostaining workflow to enable easy super-resolution imaging. 🔬 Kits available for popular tags: ·      GFP ·      Halo ·      RFP ·      ALFA ·      TagFP ·      mEOS 𝗘𝘅𝗽𝗹𝗼𝗿𝗲 𝗸𝗶𝘁𝘀: massive-photonics.com/produc… Kits include DNA-PAINT ready binders, imager strands, and buffers for imaging and sample prep. Start revealing cellular structures with nanoscale precision today! Single-domain antibodies licensed from NanoTag Biotechnologies, where they’re available unmodified and fluorophore-coupled. #DNAPAINT #SuperResolution #FluorescentProteins #SMLM #Microscopy

Researchers at the University of North Carolina Chapel Hill wanted to map 12 nuclear targets, including epigenetic marks, transcription machinery, heterochromatin proteins, and DNA at the nanoscale. 🔬nature.com/articles/s41467-0… Off-the-shelf reagents weren't going to cut it. They needed Exchange-PAINT secondary nanobodies (i.e., sdABs) with orthogonal docking sequences, minimal background in a dense nuclear environment, and high specificity. We made them a custom set. The result: a comprehensive multiplexed super-resolution map of nuclear organization that revealed how chromatin compartmentalization emerges at the nanoscale. Working on a target our standard kits don't cover? Our custom conjugations can help. Get in touch: [email protected] #DNAPAINT #Superresolution #microscopy #Bioimaging

Most microscopy techniques eventually run into the same wall: your fluorescent dyes bleach out before you can collect enough data. DNA-PAINT sidesteps this entirely. Instead of relying on a fixed dye that degrades, DNA-PAINT uses short, dye-labeled DNA strands (imager strands) that transiently bind complementary strands. Because imagers are freely diffusing in solution and continuously replenished, bleaching is a non-issue. The result: stable blinking for hours, sub-20 nm resolution, quantitative capabilities, and unlimited multi-target imaging possibilities. At Massive Photonics we aim to make DNA-PAINT accessible with imaging kits designed for real labs, not just specialist setups. Curious how it works in practice? Check out our free Beginner's Guide to DNA-PAINT 👉 massive-photonics.com/dna-pa… 📸5-target DNA-PAINT imaging using MASSIVE-SDAB-FAST 5-PLEX kit, FAST LB imagers, dye Cy3B. #DNAPAINT #SuperResolution #Microscopy #CellBiology #SMLM

𝗜𝗺𝗮𝗴𝗲 𝗹𝗮𝗿𝗴𝗲 𝗰𝗲𝗹𝗹 𝘃𝗼𝗹𝘂𝗺𝗲𝘀 𝘄𝗶𝘁𝗵𝗼𝘂𝘁 𝘀𝗮𝗰𝗿𝗶𝗳𝗶𝗰𝗶𝗻𝗴 𝘀𝘂𝗽𝗲𝗿-𝗿𝗲𝘀𝗼𝗹𝘂𝘁𝗶𝗼𝗻🔬 Classical single-molecule localization microscopy (SMLM) is constrained to TIRF and HILO illumination, limiting how much of a cell you can actually see. 𝗕𝗿𝘂𝗸𝗲𝗿 𝗩𝘂𝘁𝗮𝗿𝗮 𝗩𝗫𝗟 + 𝗠𝗮𝘀𝘀𝗶𝘃𝗲 𝗣𝗵𝗼𝘁𝗼𝗻𝗶𝗰𝘀 𝗗𝗡𝗔-𝗣𝗔𝗜𝗡𝗧 𝗸𝗶𝘁𝘀 𝗰𝗵𝗮𝗻𝗴𝗲 𝘁𝗵𝗮𝘁. Our optimized imager strands enable robust DNA-PAINT via epifluorescence illumination, so you can image entire cell volumes at nanoscale resolution via z-stacks. ✅ Full volumetric imaging — no more TIRF/HILO constraints ✅ Compatible with Bruker Vutara VXL out of the box ✅ Optimized imagers for high-contrast EPI DNA-PAINT 𝗩𝗶𝗱𝗲𝗼: B cell mitochondria imaged across an entire 10.5 µm cell volume, localization precision ~11 nm. 🔗massive-photonics.com/produc…

#ELMI2026 was a success! Thanks to everyone who stopped by our booth, discussed imaging with us, and joined our #DNAPAINT workshops with @oniHQ Until next year 🎉 🌟

🎉 New paper alert 🎉 A Nature Methods paper from Fu et al. introduces MOSAIC (Multimodal Optical Scope with Adaptive Imaging Correction), a single reconfigurable microscope that integrates light-sheet, super-resolution, label-free, and multiphoton imaging, equipped with adaptive optics. MOSAIC enables imaging across scales from nanometer-resolution subcellular structures to live multicellular organisms, with the ability to switch modalities in seconds. Congrats to all authors!! The team used our reagents for DNA-PAINT imaging of Lamin A/C and TOMM20. Read in full: nature.com/articles/s41592-0…