Discovering new intersections of light & matter. Ruth L. Kirschstein postdoc @Muirlab formerly @RovisGroup & @NicewiczLab. 1st gen immigrant 🇲🇾(he/him) #YNWA
Princeton, NJ
Joined May 2009
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Excited to share that our protein editing method from the @MuirLab is now published in @ScienceMagazine
We believe that protein transposition will enable new biochemical studies of PTMs and open up new intersections of proteins and small molecules
science.org/doi/10.1126/scie…
Nicholas Tay retweeted
Check out our latest efforts published in @ChemicalScience on characterizing and engineering the NrdJ-1 intein for protein biochemistry and chemical biology applications! Congrats to @XuanjiaYe, Josh, Christian, @thegoodnush and Juner! @PrincetonChem
Nicholas Tay retweeted
Employing engineered split inteins to replace the internal regions of target proteins in a single reaction. science.org/doi/10.1126/scie… #NBThighlight
Nicholas Tay retweeted
Excited to share our @nchembio publication! Our proximity-triggered protein trans-splicing technology enables precise, time-resolved activation of diverse proteins by manipulating control elements (1/5)
nature.com/articles/s41589-0…
Nicholas Tay retweeted
Check out our latest paper in @nchembio, where we developed our CAGE split intein platform to study signaling events using proteomic profiling. This enables insights into the differential molecular connections of fusion oncogenes. Congrats to @GihoonLee1!
nature.com/articles/s41589-0…
Late to highlight this, but excited to share the latest intein engineering work in the @MuirLab that I've been able to contribute towards. In short, we think that this protein transposition technology can help us to study the functional landscape of IDRs within folded proteins
Ever wanted to swap out an entire internal region of a folded protein for functional biochemical studies? Check out our preprint that has been 5 years in the making, in which we establish a protein editing platform via protein transposition, enabling the insertion of functional cargo or PTMs into internal folded protein regions. #proteinengineering #proteinediting
biorxiv.org/content/10.1101/…
Congrats to Yi, who has labored hard on this problem, and the rest of the team (Xuanjia, @jeremyaowen, @Hengyuan_Liu03, @rtho5616 ) for their contributions! It will be exciting to see where this technology will go in the future.
Nicholas Tay retweeted
Congratulations to our newest inductee into the @theNASciences! @PrincetonChem
Nicholas Tay retweeted
@jeremyaowen aims to expand our understanding of chromatin remodelers, complex protein machines responsible for packaging DNA and regulating gene expression.
Great to see this project go from the chalkboard during my time in the Rovis lab to completion! Congrats!
New year, new proximity labeling protocol! We can use red light to access trifluoromethyl carbenes from diazos for proximity labeling. Congrats to @crabaneros and @stavkariofillis for a great paper in collaboration with Merck!
pubs.acs.org/doi/10.1021/jac…
Excited to share some of the research I’ve been up to in the @MuirLab , where we use caged inteins as programmable sensors on the cell surface to reconstitute a SpyCatcher loading dock for cell-targeted applications. biorxiv.org/content/10.1101/…
Nicholas Tay retweeted
1/ My work on epigenetic memory with @leonidmirny, @osmanovicdino1 is out @ScienceMagazine!
science.org/doi/10.1126/scie…
How can *stable* memory be encoded in patterns of *dynamic* epigenetic marks? We find 3D folding of genome can stabilize memory, if 3 ingredients are present...
Nicholas Tay retweeted
US citizens or permanent residents from an under-represented group! High school thru postdoc/faculty.
You can contact any NIH-funded researcher you admire & suggest applying for this supplement to join their lab! Many, like me, are happy to host!
grants.nih.gov/grants/guide/…
Nicholas Tay retweeted
Check out our new pre-print, illustrating the power of our strategy for studying molecular pathways of oncofusions associated with the initial stages of cell transformation and discovering upstream intermediates as novel cancer therapeutic targets.
biorxiv.org/content/10.1101/…
Nicholas Tay retweeted
Out today in @Nature is our work in collaboration with the Chen group at CSU! Installing dynamic crosslinkers into a variety of inmiscible polymer mixtures provides a solution to closed-loop mixed plastic recycling ♻️ ✅
nature.com/articles/s41586-0…
Nicholas Tay retweeted
I'm sad to share that my student, Dr. Larry Mendoza, was struck by a car last week and remains unconcious and in critical condition. If you are able, please consider supporting him and his family via the below gofundme.
gofund.me/7f4cefb1
Nicholas Tay retweeted
Today we introduce Light-induced Interactome Tagging (LITag) in @PNASNews, where we use light to activate a genetically-encoded LOV protein for second-scale proximity labeling. Congrats to authors @HananyaNir, Xuanjia, and @ShanyKoren!
pnas.org/doi/10.1073/pnas.22…
Nicholas Tay retweeted
Looking to study chromatin interactomes with proximity labeling? Check out our new paper in @Nature, where we combine our split-intein expertise with uMap technology from the @MacMillan_Lab to map the microenvironment of chromatin state changes.
nature.com/articles/s41586-0…