@labohaley

We develop and apply advanced genetic manipulation technologies to make sense of biological complexity. Formerly @Genentech now @UMontreal.

 Montreal, Quebec, Canada
labohaley.com
Joined July 2023
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Our last pre-print of 2024! We created a simple-to-use, temporally-controlled CRISPR/Cas9 toolset that doesn’t compromise between background and induced editing. biorxiv.org/content/10.1101/…

Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation

CRISPR/Cas9 technologies provide unique capabilities for modeling disease and understanding gene-to-phenotype connections. In cultured cells, chemical-mediated control of Cas9 activity can limit...

biorxiv.org
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The product of a wonderful collaboration across @genentech led by the brilliant duo of Rajini Srinivasan and Tao Sun, with essential guidance from @andrewng_synbio and contributions from many others.
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We’ve been excited about sharing these fantastic new systems with the community (some of you had a preview at the last CSHL Genome Eng. conf.), and we would love to hear any feedback or answer any questions you might have for us.
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Have you been interested combining Perturb-Seq screening with Cas12a’s unique capabilities for multiplexed guide RNA expression? Us too! So, we figured out some useful tricks to make it possible, which you can find in our new pre-print. Short thread below: biorxiv.org/content/10.1101/…

Degron-modified Cas12a enhances single-cell CRISPR screening

Single-cell CRISPR (Perturb-seq) screens have primarily relied on Cas9 whereas Cas12a, despite its unique effectiveness for multiplex guide expression, remains underexplored. This may be due to...

biorxiv.org
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And, that worked out every bit as hoped! Shown here is one example of a known phenotype (KDM1a/LSD1 depletion = Vim and/or HLA-I upregulation). More examples in the main text and supplement.
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We hope you find this approach as useful as we did, and don’t hesitate to reach out if you have questions or comment!
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Attention gene editors! We are searching for faculty in gene editing (broadly defined) at UC Irvine: recruit.ap.uci.edu/JPF09319
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We are recruiting a colleague in the Donnelly Centre at the University of Toronto. We are looking for an assistant professor in the broad area of functional genomics: jobs.utoronto.ca/job/Toronto… closing date is Jan 16, 2025 @DonnellyCentre @UofT @uoftmedicine

Assistant Professor - Functional Genomics

Assistant Professor - Functional Genomics

jobs.utoronto.ca
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BREAKING NEWS The 2024 #NobelPrize in Physiology or Medicine has been awarded to Victor Ambros and Gary Ruvkun for the discovery of microRNA and its role in post-transcriptional gene regulation.
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My department is hiring a tenure-track faculty focusing on RNA biology! @UCIrvine has a large, vibrant and super supportive RNA community! Come join us! recruit.ap.uci.edu/JPF09320
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📢 Join us! Developmental and stem cell biologists, checkout our tenure-track faculty position at Sloan Kettering. Apply by October 18. #DevBio #StemCell #hiringnow mskcc.org/research/ski/posit…
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Consider joining our team as we work toward breakthrough #cancervaccines – The Olayan Center for Cancer Vaccines at #MSKCancerCenter is currently recruiting a Head for our RNA Therapeutics Lab, and we are always looking for talented postdocs! careers.mskcc.org/vacancies/…
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The RTI & @UMassChan Medical School seeks the next generation of scientific leaders! Join our research community as a Maraganore Early Independence Fellow. Get 3-5 years of support. Apply by Dec 1 to join us: academicjobsonline.org/ajo/j…
The goal of the UMass Chan Early Independence Fellows Program is to recruit clusters of exceptionally talented junior scientists with the potential to become independent investigators at UMass Chan Medical School, and to provide them with career development, mentoring, and financial support to foster their scientific excellence and leadership. In recognition of diversity as a driver of innovation and scientific impact, we are committed to building and sustaining an inclusive environment with talented individuals from all backgrounds, including backgrounds that are underrepresented in medicine and the biomedical sciences, who embrace diversity, equity and inclusion in line with our institutional goals (IMPACT 2025). Candidate evaluations will consider the unique scientific contributions of the candidate as well as leadership and resilience qualities the candidate brings to the program.

ALT The goal of the UMass Chan Early Independence Fellows Program is to recruit clusters of exceptionally talented junior scientists with the potential to become independent investigators at UMass Chan Medical School, and to provide them with career development, mentoring, and financial support to foster their scientific excellence and leadership. In recognition of diversity as a driver of innovation and scientific impact, we are committed to building and sustaining an inclusive environment with talented individuals from all backgrounds, including backgrounds that are underrepresented in medicine and the biomedical sciences, who embrace diversity, equity and inclusion in line with our institutional goals (IMPACT 2025). Candidate evaluations will consider the unique scientific contributions of the candidate as well as leadership and resilience qualities the candidate brings to the program.

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We're excited to initiate a search for new faculty for the UCLA Broad Stem Cell Research Center! We're seeking creative researchers to advance basic stem cell biology and translation for the next generation of stem cell therapeutics.
UCLA Broad Stem Cell@UCLAstemcell18 Sep 2024
📢 Our center is recruiting multiple new faculty working in stem cell research! Apply today to join UCLA's highly collaborative multi-disciplinary research environment: recruit.apo.ucla.edu/JPF0974…
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